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1998 Fiscal Year Final Research Report Summary

Japan-UK Joint Study on Molecular Genetics of Streptomyces

Research Project

Project/Area Number 09044205
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field 応用微生物学・応用生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

HORINOUCHI Sueharu  The University of Tokyo, Graduate Shool of Agriculture and Life Sciences Professor, 大学院・農学生命科学研究科, 教授 (80143410)

Co-Investigator(Kenkyū-buntansha) IKEDA Haruo  Kitasato university School of Pharmaceutical Sciences Associate Professor, 薬学部, 助教授 (90159632)
OGAWARA Hiroshi  Meiji College of Pharmacy Department of Biochemistry Professor, 薬学部, 教授 (00097198)
YAMADA Yasuhiro  Osaka University, Graduate School of Engineering Professor, 工学部, 教授 (00011891)
SETO Haruo  The University of Tokyo, Institute of Molecular and Cellular Biosciences Profess, 分子細胞生物学研究所, 教授 (10013335)
BEPPU Teruhiko  Nihon University Department of Applied Biological Sciences Professor, 生物資源科学部, 教授 (80011873)
Project Period (FY) 1997 – 1998
KeywordsJapan-UK Joint Study / Streptomyces / Molecular Biology / Antibiotics Production / Genetic Engineering / Molecular Breeding / Secondary Metabolism / Morphological Differentiation
Research Abstract

The purpose of this study is promoting Japan-UK joint researches on molecular genetics of Streptomyces. A workshop on molecular genetics of Streptomyces was held at John Innes research center (Norwich, UK) on October 1997. Fifteen Japanese researchers took part in the workshop and discussed about joint researches. The proceeding of the workshop was distributed to many researchers who study on Streptomyces.
In the second year, 1998, three professors of John Innes research center (Sir. D.Hopwood, K.F.Chater and M.J.Bibb) visited Japan and discussed about joint researches with Japanese researchers. S.Horinouchi, head investigator of this study, visited John Innes research center on September. Y.Ohnishi, one of inverstigators of this study, stayed for three weeks at John Innes research center. He made experiments with some researchers of the center.
Thanks to this study, many joint researches started and more intimate relationship was established between Japanese and English researchers.

  • Research Products

    (170 results)

All Other

All Publications (170 results)

  • [Publications] Ando, N.: "Involvement of AfsA in A-factor biosynthesis as a key enzyme" J. Antibiot.50. 847-852 (1997)

    • Description
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  • [Publications] Ando, N.: "A Streptomyces griseus gene (sgaA) suppresses the growth disturbance caused by high osmolality and a high concentration of A-factor during carly growth" Microbiology. 143. 2715-2723 (1997)

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  • [Publications] Lezhava, A.: "Chromosomal deletions in Streptomyces griscus that remove the afsA locus" Mol. Gen. Genet.253. 478-483 (1997)

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  • [Publications] Onaka, H.: "DNA-binding activity of the A-factor receptor protein and its recognition DNA sequences" Mol. Microbiol.24. 991-1000 (1997)

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  • [Publications] Onaka, H.: "A mutation at proline-115 in the A-factor receptor protein of Streptomyces griseus abolishes DNA-binding ability but not ligand-binding ability" J. Bacteriol.179. 2748-2752 (1997)

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  • [Publications] Kendrew, S.G.: "Identification of a monooxygenase from Steptomyces coclicolor A3(2) involved in biosynthesis of actinorbodin : purilication and characterization of the recombinant enzyme" J. Bacteriol.179. 4305-4310 (1997)

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  • [Publications] Kramer, P.J.: "Rational design and engineered biosynthesis of a novel 1.8-carbon aromatic polyketide" J. Am. Chem. Soc.119. 635-639 (1997)

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  • [Publications] Crump, M.P.: "Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Steptomyces coelicolor A3(2)" Biochemistry. 36. 6000-6008 (1997)

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  • [Publications] Hopwood, D.A.: "Genetic contributions to understanding polyketide synthases" Chemical Reviews. 97. 2465-2497 (1997)

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  • [Publications] Ueda, K.: "Stimulatory effect of copper on anibiotic production and morphological differentiation in Streptomyces tanashiensis" J. Antibiot.50. 693-695 (1997)

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  • [Publications] Aubert, M.: "Inactivation or amplification of the spa2 gene, encoding a potential stationary-phase regulator, affects differentiation in Streptomyces ambofaciens." Can. J. Microbiol.43. 1118-1125 (1997)

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  • [Publications] Martin, M.C.: "A glgC gene essential only for the first of two spatially distince phases of glycogen synthesis in Streptomyces coelicolor A3(2)" J. Bacteriol.179. 7784-7789 (1997)

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  • [Publications] Oh, S.H.: "Denaturation of circular of linear DNA facilitates targeled integralive transformation of Streptomyces coelicolor A3(2) : possible relevance to other organisms" J. Bacteriol.179. 122-127 (1997)

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  • [Publications] Nakashita, H: "Studies on yhe biosynthesis of bialaphos. Biochemical mechanism of C-P bond formation : Discovery of phosphonopyruvate decarboxylase which catalyzes the formation of phosphonoacctaldehyde from phoisphonopyruvate" J. Antibiot.50. 212-219 (1997)

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  • [Publications] 瀬戸 治男: "C-P結合形成の分子機構の解明-生物有機化学と分子生物学の接点" 日本農芸化学会誌. 71. 451-453 (1997)

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  • [Publications] 越智 幸三: "リボソームの改造による抗生物質の生産性向上" バイオサイエンスとインダストリー. 55. 35-36 (1997)

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  • [Publications] Hotta, K.: "Enzymatic 2′-N-acetylation of arbekacin and antibiotic activily of its product" J. Antibiot.49. 458-464 (1996)

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  • [Publications] Hotta, K.: "Mechanism of multiple aminoglycoside resistance of kasugamycin-producing Streptomyces kasugaensis MB273 : involbement of two types of acetyltransferases in resistance to astomicin group antibiotics" J. Antibiot.49. 682-688 (1996)

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  • [Publications] 堀田 国元: "アミノグリコシド系薬剤" 日本臨床. 55. 219-225 (1997)

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  • [Publications] Kataoka, M.: "Application of the variable region in 16S rDNA to create an index for rapid species identilication in the genus Streptomyces" FEMS Microbiol. Lett.151. 249-255 (1997)

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  • [Publications] Wietzorrek, A.: "A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA-binding fold" Mol. Microbiol.25. 1177-1184 (1997)

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  • [Publications] Chakrabutryy, R.: "The ppGpp synthetase gene (rclA) of Streptomyces coelicolor A3(2) plays a conditional role in antibiotic production and morphological differentiation" J. Bacteriol.179. 5854-5861 (1997)

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  • [Publications] Labes, G.: "Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage 119 using the gentamicin resistance gene (aacCl) of Tn 1696 as reporter" Microbiology. 143. 1503-1512 (1997)

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  • [Publications] Tieleman, L.: "Growth phase-dependent transcription of the Streptomyces ramocissimus tufl gene occurs from two promoters" J. Bacteriol.179. 3619-3624 (1997)

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  • [Publications] van Wezel, G.P.,: "The malEFG gene eluster of Streptomyces coelicolor A3(2) : characterization, disruption and transcriptional analysis" Mol. Gen. Genet.254. 604-608 (1997)

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  • [Publications] Alves, A.N.C.R.: "Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic parhway of the actinomycete Streptomyces coelicolor A3(2)" Appl. Environ. Microbiol.63. 956-961 (1997)

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  • [Publications] van Wezel, G.P.: "Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) controlled by malR, a member of the lacl-galR family of regulatory genes" Mol. Microbiol.23. 537-549 (1997)

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  • [Publications] White, J.: "bldA dependence of undeeylprodigiosin production in Streptomyces coelicolor A3(2) involves a pathway-specific regulatory cascade" J. Bacteriol.179. 627-633 (1997)

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  • [Publications] Ikeda, H.: "A vermectin biosynthesis" Chemical Reviews. 97. 2591-2609 (1997)

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  • [Publications] Zhang, H.: "Improvement of transformation system is Streptomyces using a modified regencration medium" J. Ferment. Bioeng.83. 217-221 (1997)

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  • [Publications] 小河原 宏: "最前線:放線菌遺伝子の発現調節、原核生物型?真核生物型?" ファルマシア. 33. 150-154 (1997)

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  • [Publications] Kawachi, R.: "Development of a Transformation System in Streptomyces virginiac" FEMS Microbiol. Lett.157. 46-53 (1997)

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  • [Publications] Kawabuchi, M.: "Production of butyrolactone autoregulators by Streptomyces coelicolor A3(2)" FEMS Microbiol. Lett.157. 81-85 (1997)

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  • [Publications] Waki, M.: "Cloning and characterization ofthe gene (farA) encoding the receptor for an extracellular regulatory factor (IM-2) from Streptomyces sp. strain FRI-5" J. Bacteriol.179. 5131-5137 (1997)

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  • [Publications] Kinoshita, H.: "Butyrolactone autoregulator receptor protein (BarA) as a transcriptional regulator in Streptomyces virginiac" J. Bacteriol.179. 6986-6993 (1997)

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  • [Publications] Okamoto, S.: "Molecular cloning and characterization of the obg gene of Streptomyces griscus in relation to the onset of morphological differentiation" 179. 170-179 (1997)

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  • [Publications] 細谷 仁子: "微生物の潜在能力を引き出す-新規物質の探索、次代謝産物生産菌の生産性向上に新手法-" 化学と生物. 35. 684-686 (1997)

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  • [Publications] Kawamoto, S.: "Expression analysis of the ssgA gene product, associated with sporulation and cell division in Streptomyces griscus" Microbiol.143. 1077-1086 (1997)

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  • [Publications] Hesketh, A.: "A novel method for improving Streptomyces coelicolor A3(2) for production of actinorhodin by introduction of rpsL (encoding ribosomal protein S12) mutations conferring resistance to streptomycin" J. Antibiot.50. 532-535 (1997)

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  • [Publications] Shima, J.: "Novel detoxification of the trichothecene mycotoxin deoxynivalcnol by a soil bacterium isolated by enrichment culture" Appl. Env. Microbiol.63. 3825-3830 (1997)

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  • [Publications] Kawamaot, S.: "Molecular analysis of the ribosomal L11 protein gene (rplK=relC) of Streptomyces griseus and identification of a deletion allele" Mol. Gen. Genet. .255. 549-560 (1997)

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  • [Publications] Ochi, K.: "Motecular and functional analysis of the ribosomal L11 and S12 protein genes (rplK and rpsL) of Streptomyces coelicolor A3(2)" Mol. Gen. Genet. .256. 488-498 (1997)

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  • [Publications] Sugiyama, M.: "Site-directed mutagenesis of the A-factor receptor protein : Val-41 important for DNA-binding and Trp-119 important for ligand-binding" Gene. 222. 133-144 (1998)

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  • [Publications] Ueda, K: "Characterization of an A-factor-responsive repressor for amfR esscntial for onset of acrial mycelium formation in Streptomyces griseus" J. Bacteriol.180. 5085-5093 (1998)

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  • [Publications] Onaka, H.: "Involvement of two A-factor receptor homologues in Streptomyces coelicolor A3(2) in the regulation of seeondary metabolism and morphogenesis" Mol. Microbiol.28. 743-753 (1998)

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  • [Publications] Ando, N.: "Gene organization in a 12-kilobase region covering afsA eneoding an A-factor biosynthetic enzyme" Actinomycetol.12. 40-45 (1998)

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  • [Publications] Hong, S.-K.: "Effects of protein kinase inhibitors on in vitro protein phosphorylation and on secondary metabolism and morphogenesis in Streptomyces coelicolor A3(2)" J. Microbiol. Biotechnol.8. 325-332 (1998)

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  • [Publications] Kang, D.-K.: "Possible involvement of cAMP in aerial mycclium formation and secondary mctabolism in Streptomyces griseus" Microbiology. in press. (1999)

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  • [Publications] Tin-Wein Yu: "Engineered biosynthesis of novel polyketides from Streptomyces spore pigment polyketide synthases" J. Am. Chem. Soc.120. 7749-7759 (1998)

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  • [Publications] Chong, P.P.: "Physical identification of a chromosomal locus encoding bisynthetie genes for the lipopeptide calcium-dependent antiblotic (CDA) of Streptomyces coelicolor A3(2)" Microbiology. 144. 193-199 (1998)

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  • [Publications] Tropf, S.: "Heterologously expressed acyl carrier protein domain of rat fally acid synthase functions in Escherichia coli fally acid synthase and Streptomyces coelicolor polyketide wvnthase systems" Chemistry & Biology. 5. 135-146 (1998)

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  • [Publications] Ichinose, K.: "The granatiein biosynthetic gene cluster of Streptomyces violaccoruber Tu22 : sequence analysis and expression in a heterologous host" Chemistry & Biology. 5. 647-659 (1998)

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  • [Publications] Ryding, N.J.: "A developmentally regulated gene encoding a repressor-like protein is essential for sporulation in Streptomyces coelicolor A3(2)" Mol. Microbiol.29. 343-357 (1998)

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  • [Publications] Tan, H.: "The Streptomyces coelicolor sporulation-specific σWhiG form of RNA polymerase transcribes a gene encoding a ProX-like protein that is dispensable for sporulation" Gene. 212. 137-146 (1998)

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  • [Publications] Ikeda, H.: "Cloning of the gene encoding avermeetin B 5-O-methyltransferase in avermeetin-producing Streptomyces avermililis" Gene. 206. 175-180 (1998)

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  • [Publications] Guthrie, E.P.: "A response-regulator-like activator of antibiotic synthesis from Streptomyces coelicolor A3(2) with an amino-terminal domain that lacks a phosphorylation pocket" Microbiology. 144. 727-738 (1998)

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  • [Publications] Kang, S.G.: "Actinorhodin and undeeylprodigiosin production in wild-type and relA mutant strains of Streptomyces coelicolor A3(2) grown in continuous culture" FEMS Microbiol. Lett.168. 221-226 (1998)

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  • [Publications] Redenbach, M.: "Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCPl" J. Bacteriol.180. 2796-2799 (1998)

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  • [Publications] Kinashi, H.: "Physical mapping of the linear plasmid pSLA2-L and localization of the cryAl and act1 homologs" Biosci. Biotechnol. Biochem.62. 1892-1897 (1998)

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  • [Publications] Hirakata, T.: "Putative protein serine/threonine kinase genes are located in several positions on the chromosome of Streptomyces coelicolor A3(2)." FEMS Microbiol Lett.159. 1-5 (1998)

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  • [Publications] Itohara, T.: "Prelininary protecome analysis of membrane proteins in Streptomyces coelicolor A3(2)" Actinomycetologica. in press. (1999)

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  • [Publications] Nakano, H.: "Gene replacement analysis of the Streptomyces vigineae barA gene encoding the bulyolactlanc autoregulaior receptor revealstha BarA acis as a repressor in virginiamycin biosynthesis" J. Bacteriol.180. 3317-3322 (1998)

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  • [Publications] Suzuki, N.: "Purification and characterization of virginiamycin Ml reductase from Stepromyces virginiac" Antimicrob. Agents Chemother.42. 2985-2988 (1998)

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  • [Publications] Okamoto, S.: "In vitro protein phosphorylation associated with cellular differentiation of Streptomyces griseus" Arch. Microbiol.169. 174-177 (1998)

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  • [Publications] Ochi, K.: "Genetic mapping and characterization of novel mutations which suppress the effect of a relC mutation on antibiotic production in Streptomyces coelicolor A3(2)" J. Antibiot.51. 592-595 (1998)

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  • [Publications] Kawamoto, S.: "Comparative ribosomal protein (L11 and L30) sequence analyses of several Streptomyces spp. commonly used in genetic studies" Int. J. Sys. Bacteriol.48. 597-600 (1998)

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  • [Publications] Chater, K.F.: "Taking a genetic scalpet to the Streptomyecs colony" Microbiology. 144. 1465-1478 (1998)

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  • [Publications] Elliot, M.: "The bldD gene of Streptomyecs coelicolor A3(2) : a regulatory gene involved in morphogenesis and antibiotic production" J. Bacteriol.180. 1549-1555 (1998)

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  • [Publications] Kelemen, G.: "Developmental regulation of transcription of whiE, a locus specifying the polyketide spore pigment in Streptomyecs coelicolor A3(2)" J. Bacteriol.180. 2515-2521 (1998)

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  • [Publications] Takahashi, S.: "A l-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-eryth-ritol 4-phosphate in an alternative nonmevalonatc pathway for terpen" Proc. Natl. Acad. Sci. USA. 95. 9879-9884 (1998)

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  • [Publications] Kazuyama, T.: "Direct formation of 2-methyl-D-crythritol 4-phosphate from l-deoxy-D-xylulose 5-phosphate by l-deoxy-D-xylu-lose 5-phosphate reductoisomerase, a new enzyme in the non-mevaionate pathway to isopentenyl diphosphate" Terahedron Lett.39. 4509-4512 (1998)

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  • [Publications] Orihara, N.: "Studies on the biosynthesis of terpenoid compounds produced by actinomyectes. 3. Biosynthesis of the isoprenoid side chain of novobiocin via the non-mevalonate pathway in Streptomyces niveus" J. Antibiot.51. 676-678 (1998)

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  • [Publications] Kuzuyama, T.: "Fosmidomyein, a specific inhibitor of l-deoxy-D-xylulose 5-phosphate reductoisomerase in the nonmvalonate pathway for terpenoid biosynthesis" Tetrahedron Lett.39. 7913-7916 (1998)

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  • [Publications] Seto, H.: "Studies on the biosynthesis of terpenoids produced by actinomycctes. Part 4. Formation of BE-40644 by the mevalonate and nonmcvalonate pathways" Terahedron Lett.39. 9497-9500 (1998)

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  • [Publications] Takahashi, S.: "Purification, characterization, and cloning of a eubacteral 3-hydroxy-3-methylglutaryl coenzyme A reductase, a key enzyme involved in biosynthesis of terpenoids" J. Bacteriol.in press. (1999)

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  • [Publications] Kuzuyama, T.: "Construction and characterization of Escherichia coli disruptants defective in the yaeM gene" Biosci, Biotechnol. Biochem.in press. (1999)

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  • [Publications] Hotta, K.: "The novel enzymatic 3″-N-acetylation of arbekacin by an aminoglyeoside 3-N-acctyltransferase of Streptomyces origin and the resulting activity" J. Antibiot.51. 735-742 (1998)

    • Description
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  • [Publications] Ueda, K.: "Two distinet mechanisms cause heterogencity of 16S rDNA" J. Bacteriol.181. 78-82 (1999)

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  • [Publications] Hosoya, Y.: "Acquisition of certain streptomyein-resistant (str) mutations enhances antibiotie production in bacteria" Antimicrob. Agents Chemother.42. 2041-2047 (1998)

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  • [Publications] Okamoto, S.: "An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor" Mol. Microbiol.30. 107-119 (1998)

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  • [Publications] Kawamoto, S.: "Sequence analysis of the ribosomal L11 protein gene (rplK=relC) in Streptomyces lavendulac using a deletion allele" J. Antibiot.51. 954-957 (1998)

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  • [Publications] Han, W.-D.: "A novel sporulation-control gene (spoOM) of Bacilus subtilis with a σ_<ll>-regulated promoter" Gene. 217. 31-40 (1998)

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  • [Publications] Ichinose, K.: "Biosynthetic gene cluster of benzoisochromanequinone antibioties in Streptomyces spp : identification of genes involved in post-PKS tailoring steps" Actinomycetol.12. 99-109 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ichinose, K.: "Proof that the actVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific phran ring formation in the biosynthesis of actinorhodin" Bioorg. Med. Chem. Lett.9. 395-400 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡本 晋: "微生物の形態分化に関与するGTP結合タンパク質" 生物工学会誌. 76. 72-75 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 瀬戸 治男: "微生物が行うユニークな化学反応-C-P化合物の生合成機構" 日本農芸化学会誌. 72. 1-18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川本 伸一: "放線菌における二次代謝と緊縮制御" 化学と生物. 36. 144-145 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 堀田 国元: "放線菌のアミノグリコシド抗生物質多剤耐性とその応用" 生物工学会誌. 76. 76-81 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 堀田 国元: "アミノ配糖体の耐性機序" 医薬ジャーナル. 34. 65-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田 治生: "コンビナトリアル・バイオシンセシス〜ポリケチド化合物を例として" 蛋白質・核酸・酵素. 43. 49-61 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木梨 陽康: "放線菌の線状染色体のダイナミックな構造変化と染色体の起源・進化" 蛋白質・核酸・酵素. 43. 1453-1460 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horinouchi, S.: "Dynamic Aspects of Natural Products Chemistry ; Molecular Biological Approaches" U.Sankawa and K. Ogura (eds. ) Kodansha and Harwood Academic Publishers, Tokyo and Amsterdam, 296 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horinouchi, S.: "Cell-Cell Communication in Bacteia" G. M. Dunny and S. C. Winans (eds.) American Society for Microbiology, Washington, D. C. (in press), (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando, N., et.al.: "Involvement of AfsA in A-factor biosynthesis as a key enzyme" J.Antibiot.50. 847-852 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, N., et.al.: "A Streptomyces griseus gene (sgaA) suppresses the growth disturbance caused by high osmolality and high concentration of A-factor during early growth" Microbiology. 143. 2715-2723 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lezhava, A., et.al.: "Chromosomal deletions in Streptomyces griseus that remove the afsA locus" Mol.Gen.Genet.253. 478-483 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Onaka, H.et., al.: "DNA-binding activity of the A-factor receptor protein and its recognition DNA sequences" Mol.Microbiol.24. 991-1000 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Onaka, H.et., al.: "A mutation at proline-115 in the A-factor receptor protein of Streptomyces griseus abolishes DNA-binding ability but not ligand-binding ability" J.Bacteril.179. 2748-2752 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hong, S.-K.et., al.: "Effects of protein kinase inhibitors on in vitro protein phosphorylation and on secondary metabolism and morphogenesis in Streptomyces coelicolor A3 (2)" J.Microbiol.Biotechnol.8. 325-332 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, K., et.al.: "Characterization of an A-factor-responsive repressor for amtR essential for onset of aerial mycelium formation in Streptomyces griseus" J.Bacteriol.180. 5085-5093 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Onaka, H.et.al.: "Involvement of two A-factor recptor homologues in Streptomyces coelicolor A3 (2) in the regulation of secondary metabolism and morphogenesis" Mol.Microbiol.28. 743-755 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando, N., et.al.: "Gene organization in a 12-kilobase region covering afsA encoding an A-factor biosynthetic enzyme" Actinomycetol.12. 40-45 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugiyama, M., et.al.: "Site-directed mutagenesis of the A-factor receptor protein : Val-41 important for DNA-binding and Trp-119 important for ligand-binding" Gene. 222. 133-144 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kang, D.-K., et.al.: "Possible involvement of cAMP in aerial mycelium formation and secondary metabolism in Streptomyces griseus" Microbiology. in press. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kendrew, S.G., et.al.: "Identification of a monooxygenase from Streptomyces coelicolor A3 (2) involved in biosynthesis of actinorhodin : purification and characterization of the recombinant enzyme" J.Bacteriol.179. 4305-4310 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kramer, P.J., et.al.: "Rational design and engineerd biosynthesis of a novel 18-carbon aromatic polyketide" J.Am.Chem.Soc.119. 635-639 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Crump, M.P., et.al.: "Solution structure of the actinorhodin polyketide synthase acyl carrier prtein from Streptomyces coelicolor A3 (2)" Biochemistry. 36. 6000-6008 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hopwood, D.A.: "Genetic contributions to understanding polyketide synthases" Chemical Reviews. 97. 2465-2497 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, K., et.al.: "Stimulatory effect of copper on antibiotic production and morphological differentiation in Streptomyces tanashiensis" J.Antibiot.50. 693-695 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aubert, M., et.al.: "Inactivation or amplification of the spa2 gene, encoding an potential stationary-phase regulator, affects differentiation in Streptomyces ambofaciens" Can.J.Microbiol.43. 1118-1125 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Martin, M.C., et.al.: "A glgC gene essential only for the first of two spatially distince phases of glycogen synthesis in Streptomyces coelicolor A3 (2)" J.Bacteriol.179. 7784-7789 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oh, S.H., et.al.: "Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3 (2) : possible relevance to other organisms" J.Bacteriol.179. 122-127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakashita, H., et., al.: "Studies on yhe biosynthesis of bialaphos.Biochemical mechanism of C-P bond formation : Discovery of phosphonopyruvate decarboxylase which catalyzes the formation of phosphonoacetaldehyde from phosphonopyruvate" J.Antibiot.50. 212-219 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka, M., et., al.: "Application of the variable region in 16S rDNA to create an index for rapid species identification in the genus Streptomyces" FEMS Microbiol.Lett.151. 249-255 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wietzorrek, A., et.al.: "A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA-binding fold" Mol.Microbiol.25. 1177-1184 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chakraburtty, R., et.al.: "The ppGpp synthetase gene (relA) of Streptomyces coelicolor A3 (2) plays a conditional role in antibiotic production and morphological differentiation" J.Bacteriol.179. 5854-5861 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Labes, G., et.al.: "Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage 119 using the gentamicin resistance gene (aacCl) of Tn 1696 as reporter" Microbiology. 143. 1503-1512 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tieleman, L., et.al.: "Growth phase-dependent transcription of the Streptomyces ramocissimus tuf1 gene occurs from two promoters" J.Bacteriol.179. 3619-3624 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] van Wezel, G.P.et.al.: "The malEFG gene cluster of Streptomyces coelicolor A3 (2) : characterization, disruption and transcriptional analysis" Mol.Gen.Genet.254. 604-608 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Alves, A.M.C.R., et.al.: "Identification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the actinomycete Streptomyces coelicolor A3 (2)" Appl.Environ.Microbiol.63. 956-961 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] van Wezel, G.P., et.al.: "Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3 (2) is controlled by malR.a member of the lacl-galR family of regulatory genes" Mol.Microbiol.23. 537-549 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] White, J., et.al.: "bldA dependence of undecylprodigiosin production in Streptomyces coelicolor A3 (2) involves a pathway-specific regulatory cascade" J.Bacteriol.179. 627-633 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda, H., et.al.: "Avermectin biosynthesis" Chemical Reviews. 97. 2591-2609 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhang, H., et.al.: "Improvement of tranformation system in Streptomyces using a modified regeneration medium" J.Ferment.Bioeng.83. 217-221 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawachi, R., et.al.: "Development of a Transformation System in Streptomyces virginiae" Actinomycetologica. 11. 46-53 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawabuchi, M., et.al.: "Production of butyrolactone autoregulators by Streptomyces coelicolor A3 (2)" FEMS Microbiol.Lett.157. 81-85 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Waki, M., et.al.: "Cloning and characterization of the gene (farA) encoding the receptor for an extracellular regulatory factor (IM-2) from Streptomyces sp.strain FRI-5" J.Bacteriol.179. 5131-5137 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita, H., et.al.: "Butyrolactone autoregulator receptor protein (BarA) as a transcriptional regulator in Streptomyces virginiae" J.Bacteriol.179. 6986-6993 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, S., et.al.: "Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation" J.Bacteriol.179. 170-179 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto, S., et.al.: "Expression analysis of the ssgA gene product, associated with sporulation and cell division in streptomyces griseus" Microbiology. 143. 1077-1086 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hesketh, A., et.al.: "A novel method for improving Streptomyces coelicolor A3 (2) for production of actinorhodin by by introduction of rpsL (encoding ribosomal protein S12) mutations conferring resistance to streptomycin" J.Antibiot.50. 532-535 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shima, J., et.al.: "Novel detoxification of the trichothecene mycotoxin deoxynivalenol by a soil bacterium isolated by enrichment culture" Appl.Environ.Microbiol.63. 3825-3830 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto, S., et.al.: "Molecular analysis of the ribosomal L11 protein gene (rplK=relC) of Streptomyces griseus and identification of a deletion allele" Mol.Gen.Genet.255. 549-560 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ochi, K., et.al.: "Molecular and functional analysis of the ribosomal L11 and S12 protein genes (rplK and rpsL) of Streptomyces coelicolor A3 (2)" Mol.Gen.Genet.256. 488-498 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu, T.-W., et.al.: "Engineered biosynthesis of novel polyketides from Streptomyces spore pigment polyketide synthases" J.Am.Chem.Soc.120. 7749-7759 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chong, P.P., et.al.: "Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3 (2)" Microbiology. 144. 193-199 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tropf, S., et.al.: "Heterologously expressed acyl carrier protein domain of rat fatty acid synthase functions in Escherichia coli fatty acid synthase and Streptomyces coelicolor polyketide synthase systems" Chemistry & Biology. 5. 135-146 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichinose, K., et.al.: "The granaticin biosynthetic gene cluster of Streptomyces Violaceoruber Tu22 : sequence analysis and expression in a heterologous host" Chemistry & Biology. 5. 647-659 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryding, N.J., et.al.: "A developmentally regulated gene encoding a repressor-like protein is essential for sporulation in Streptomyces coelicolor A3 (2)" Mol.Microbiol.29. 343-357 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tan, H., et.al.: "The Streotimyces coelicolor sporulation-specific sWhiG form of RNA polymerase transcribes a gene encoding a ProX-like protein that is dispensable for sporulation" Gene. 212. 137-146 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chater, K.F.: "Taking a genetic scalpel to the Streptomyces colony" Microbiology. 144. 1465-1478 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Elliot, M., et.al.: "The bldD gene of Streptomyces coelicolor A3 (2) : a regulatory gene involved in morphogenesis and antibiotic production" J.Bacteriol. 180. 1549-1555 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kelemen, G., et.al.: "Developmental regulation of transcription of whiE,a locus specifying the polyketide spore pigment in Streptomyces coelicolor A3 (2)" J.Bacteriol.180. 2515-2521 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, S., et.al.: "A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpen" Proc.Natl.Acad.Sci.USA. 95. 9879-9884 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuzuyama, T., et.al.: "Direct formation of 2-C-methyl-D-erythritol 4-phosphate from 1-deoxy-D-xylulose 5-phosphate by 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a new enzyme in the non-mevalonate pathway to isopentenyl diphosphate" Tetrahedron Lett.39. 4509-4512 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Orihara, N., et.al.: "Studies on the biosynthesis of terpenoid compounds produced by actinomycetes.3.Biosynthesis of the isoprenoid side chain of novobiocin via the non-mevalonate pathway in Streptomyces niveus" J.Antibiot.51. 676-678 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuzuyama, T., et.al.: "Fosmidomycin, a specific inhibitor of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in the nonmevalonate pathway for terpenoid biosynthesis" Tetrahedron Lett.39. 7913-7916 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seto, H., et.al.: "Studies on the biosynthesis of terpenoids produced by actinomycetes.Part 4.Formation of BE-40644 by the mevalonate and nonmevalonate pathways" Tetraheron Lett.39. 9497-9500 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, S., et.al.: "Purification, characterization, and cloning of a eubacterial 3-hydroxy-3-methylglutaryl coenzyme A reductase, a key enzyme involved in biosynthesis of terpenoids" J.Bacteriol.181 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuzuyama, T., et.al.: "Construction and characterization of Escherichia coli disruptants defective in the yaeM gene" Biosci.Biotechnol.Biochem.in press. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hotta, K., et.al.: "The novel enzymatic 3"-N-acetylation of arbekacin by an aminoglycoside 3-N-acetyltransferase of Streptomyces origin and the resulting activity" J.Antibiot.51. 735-742 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, K., et.al.: "Two distinct mechanisms cause heterogeneity of 16S rRNA" J.Bacteriol.181. 78-82 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda, H., et al.: "Cloning of the gene encoding avermectin B5-O-methyltransferase in avermectin-producing Streptomyces avermitilis" Gene. 206. 175-180 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Guthrie, E.P., et.al.: "A response-regulator-like activator of antibiotic synthesis from Streptomyces coelicolor A3 (2) with an amino-termianl domain that lacks a phosphorylation pocket" Microbiology. 144. 727-738 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kang, S.G., et.al.: "Actinorhodin and undecylprodigiosin production in wild--type and relA mutant strains of Streptomyces coelicolor A3 (2) grown in continuous culture" FEMS Microbiol.Lett.168. 221-226 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Redenbach, M., et.al.: "Cloning and physical mapping of the EcoRI fragments of the giant linear plasmid SCP1" J.Bacteriol.180. 2796-2799 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinashi, H., et.al.: "Physical maping of the linear plasmid pSLA2-L and localization of the eryA1 and actI homologs" Biosci.Biotechnol.Biochem.62. 1892-1897 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirakata, T., et.al.: "Putative protein serine/threonine kinase genes are located in several positions on the chromosome of Streptomyces coelicolor A3 (2)" FEMS Microbiol.Lett.159. 1-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itohara, T., et.al.: "Preliminary proteome analysis of membrane proteins in Streptomyces coelicolor A3 (2)" Actinomycetol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano, H., et.al.: "Gene replacement analysis of the Streptomyces virgineae barA gene encoding the butyrolactone autoregulator receptor reveals that BarA acts as a repressor in virginiamycin biosynthesis" J.Bacteriol.180. 3317-3322 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N., et.al.: "Purification and characerization of virginiamycin M1 reductase from Streptomyces virginiae" Antimicrob.Agents Chemother. 42. 2985-2988 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, S., et.al.: "In vitro protein phosphorylation associated with cellular differentiation of Streptomyces griseus" Arch.Microbiol.169. 174-177 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ochi, K., et.al.: "Genetic mapping and characterization of novel mutations which suppress the effect of a relC mutation on antibiotic production in Streptomyces coelicolor A3 (2)" J.Antibiot.51. 592-595 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto, S., et.al.: "Comparative ribosomal protein (L11 and L30) sequence analyses of several Streptomyces spp.commonly used in genetic studies." Int.J.Sys.Bacteriol.48. 597-600 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoya, Y., et.al.: "Acquisition of certain streptomycin-resistant (str) mutations enhances antibiotic production in bacteria" Antimicrob.Agents Chemother. 42. 2041-2047 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, S., et.al.: "An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor" Mol.Microbiol.30. 107-119 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto, S., et.al.: "Sequence analysis of the ribosomal L11 protein gene (rplK=relC) in Streptomyces lavendulae using a delection allele" J.Antibiot.51. 954-957 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Han, W.-D., et.al.: "A novel sporulation-control gene (spoOM) of Bacillus subtilis with a sH-regulated promoter" Gene. 217. 31-40 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichinose, K., et.al.: "Biosynthetic gene cluster of benzoisochromanequinone antibiotics in Streptomyces spp : identification of genes involved in post-PKS tailoring steps" Actinomycetol.12. 99-109 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichinose, K., et.al.: "Proof that the actVI genetic region of Streptomyces coelicolor A3 (2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin" Bioorg.Med.Chem.Lett.9. 395-400 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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