1999 Fiscal Year Final Research Report Summary
Molecular Mechanisms Underlying Sperm-Eng Interaction
| Project/Area Number |
09044210
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HOSHI Motonori Tokyo Institute of Technology, Professor, 生命理工学部, 教授 (20012411)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Midori Tokyo Institute of Technology, Assistant Prof., 生命理工学部, 助手 (00211574)
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| Project Period (FY) |
1997 – 1999
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| Keywords | fertilization / acrosome reaction / sperm / oocyte / cGMP |
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| Research Abstract |
In starfish Asterias amurensis, three components in the jelly coat of eggs, namely acrosome reaction-inducing substance (ARIS), Co-ARIS and asterosap, act in concerted on homologous spermatozoa to elicit the acrosome reaction species-specifically. ARIS is proteoglycan-like compound which binds to the receptor localized to a small domain in the anterior portion of sperm heads. Its activity is mostly attributable to a sulfatedpolysaccharide chain composed of one thousand or so repeating units of a sulfated pentasaccharide. Co-ARIS is a group of saponins consisting of a sulfated steriod and a pentasaccharide. In combination with ARIS, it increases intracellular CaィイD12+ィエD1 concentration of sperm. Asterosap is a group of glutamine-rich tetratricontapeptides with a disulfide linkage. It increases cGMP level and intracellular pH of sperm through binding to its receptor that is the guanylate cyclase of 125 kDa in a major sperm membrane protein.
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