1998 Fiscal Year Final Research Report Summary
Studies on Epstein-Barr virus vector for human gene therapy
| Project/Area Number |
09044246
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Virology
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKADA Kenzo Hokkaido University school of Medicine, Professor, 医学部, 教授 (30133721)
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| Co-Investigator(Kenkyū-buntansha) |
MARUO Seiji Hokkaido University School of Medicine・Assistant, 医学部, 助手 (70292018)
IMAI Shousuke Hokkaido University School of Medicine・Assistant professor, 医学部, 助教授 (60232592)
サダデン ビル ウイスコンシン大, マカードル研究所, 副所長兼教授
ハンマーシュミット ウォ ドイツ臨床分子生物学, 腫瘍遺伝子研究所, 部長
WOLFGANG Hammerschmidt Institute of Moleculerbiology and Tumorgenetics ・ Chief
SUGDEN Bill McArdle Laboratory, Wisconsin University・Assistantmanager, Professor
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| Project Period (FY) |
1997 – 1998
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| Keywords | EB virus / Gene therapy / Viral vector |
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| Research Abstract |
There has been no infection system such as that winch produces viruses following Epstein-Barr virus (EBV infection, Therefore, it has not been possible to produce a large amount of recombinant EBV for gene therapy. In this study, we aimed to generate an amplicon system of EBV.DNA sequences containing the replication origin in a virus replicative phase and packaging signals are efficiently amplified and packaged into virus particles during virus replication. We generated cells infected with recombinant EBV with more than 200kbp genome size. Such large EBV genomes exceed the size limitation for being packaged into virus particles. Therefore, such cells should become an ideal packaging cells for propagating amplicon virus. We confirmed that Akata cells carrying huge EBV genome produce lyric antigen but do not produce progeny viruses upon treatment with anti-lg antibodies. Base on these results, we genierated a plasmid containing the replication origin and packaging signal of EBV and GFP gene, and transfected it into the Akata cells carrying huge EBV genome. We confirmed that the transfected plasmid is packaged and produced as infectious viruses after anti-lg treatment.
Dr.Takada and Dr.lmai visited Dr.Hammerschmidt's laboratory in June, 1998. During its visit, we discussed about mutual experimental results, and obtained many important informations each other.
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