1997 Fiscal Year Final Research Report Summary
Human GalNAc Transferase Isozyme Expression in Cancer Cells
| Project/Area Number |
09044318
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Gastroenterology
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| Research Institution | Kochi Medical School |
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Principal Investigator |
NISHIMORI Isao Kochi Medical School, First Department of Internal Medicine, Assistant Professor, 医学部, 助手 (30237747)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Masanori Kochi Medical School First Department of Internal Medicine, Assistant Professor, 医学部附属病院, 助手 (30191034)
ENZAN Hideaki Kochi Medical School, First Department of Pathology, Professor, 医学部, 教授 (00034645)
HOLLINGSWORT マイケル A. ネブラスカ州立大学, メディカルセンター・エプリー癌研究所, 助教授
HOLLINGSWORTH Michael A. University of Nebraska Medical Center, Eppley Cancer Institute, Associate Profes
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| Project Period (FY) |
1997
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| Keywords | N-acetylgalactosamine / Glycosyltransferase / Immunohistochemistry / mRNA / Gastric cancer / Colon cancer / Breast cancer / Pancreas cancer |
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| Research Abstract |
1.Polyclonal antibodies against two isozymes of the human UDP-N-acetylgalactosamine : polypeptide N-acetylgalactosaminyltransferase (T1 and T2) were produced by immunizations of rabbits with synthetic peptides. Histological expression and localization of T1 and T2 in normal and cancer colon tissues were studied by immunohistochmical analysis with these antibodies. In cancer tissuses, T1 and T2 expressions were observed in 1 (8%) and 9 (69%) out of 13 patients with colon cancer, respectively. In normal counterpart of colon tissues in 6 patients with colon cancer and in normal colon tissues in 20 subjects, neither T1 nor T2 expression was observed. These results showed the heterogeneous and up-regulated expression of T2 protein during carcinogenesis of colon epithelial cells.
The levels of mRNA expression of three UDP-N-acetylgalactosamine : polypeptide N-acetylgalactosaminyltransferase (T1, T2, and T3) were quantified for human adenocarcinoma cell lines from pancreas, colon, stomach, and breast. T1 and T2 were expressed constitutively and at low levels in most or all cell lines examined. T3 was differentially expressed. Well-differentiated adenocarcinoma cell lines expressed high levels and moderately differetiated cell lines expressed lower levels of T3. Cell lines classified as poorly differentiated failed to express T3 mRNA at levels that could be detected by Northern blot analysis. Differential expression of the T3 protein was confirmed in these cell lines by Western blotting. We propose that glycosylation in tumor cell lines may be regulated in part by differential expression of UDP-N-acetylgalactosamine : polypeptide N-acetylgalactosaminyltransferase.
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