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1998 Fiscal Year Final Research Report Summary

Gene cascades in cell differentiation and plasticity

Research Project

Project/Area Number 09044323
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field General medical chemistry
Research InstitutionKumamoto University

Principal Investigator

MORI Masataka  Kumamoto University, School of Medicine, Professor, 医学部, 教授 (40009650)

Co-Investigator(Kenkyū-buntansha) DAS Atze T.  Amsterdam University, Academic Medical Center, Associate Professor, 医学センター, 助教授
LAMARS Wouter H.  Amsterdam university, Academic Medical Center, Professor, 医学センター, 教授
GOTOH Tomomi  Kumamoto University, Assistant Professor, 医学部, 助手 (20264286)
TERADA Kazutoyo  Kumamoto University, Lecturer, 医学部, 講師 (00253724)
TAKIGUCHI Masaki  Chiba University, School of Medicine, Associate Professor, 医学部, 助教授 (40179578)
Project Period (FY) 1997 – 1998
Keywordsnitric oxide / nitric oxide synthase / central nervous system / astrocyte / lipopolysaccharide / Immunostaining method
Research Abstract

There are three isoforms of nitric oxide synthase (NOS), neuronal nNOS, inducible iNOS and enclotherial eNOS.eNOS and nNOS are expressed constitutively in CNS and are involved in neuronal activities and neuronal degenerative diseases. Regional distribution of neurons expressing nNOS mRNA in the rat brain was examined by non-radioisotope in situ hybridization, using digoxigenin-labeled complementary RNA probes. Clustering of intensely positive neurons was observed in discrete areas including the main and accessory olfactory bulbs, the islands of Calleja, the amygdala, the paraventricular nucleus of the thalamus, several hypothalamic nuclei, the lateral geniculate nucleus, the magnocellular nucleus of the posterior commissure, the superior and inferior colliculi, the laterodorsal and pedunculopontine tegmental nuclei, the nucleus of the trapezoid body, the nucleus of the solitary tract and the cerebellum. Strongly-stained isolated neurons were scattered mainly in the cerebral cortex, the basal ganglia and the brain stem, especially the medulla reticular formation. In the hyppocampus, an almost uniform distribution of moderately stained neurons was observed in the granular cell layer of the dentate gyrus and in the pyramidal cell layer of the ammon's horn, while more intensely stained isolated neurons were scattered over the entire hippocampal region. These observations can serve as a good basis for studies on function and gene regulation of neuronal nitric oxide synthase.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Iwase, K.,et al.: "Precise distribution of neuronal nitric oxide synthase mRNA in the rat brain revealed by non-radioisotopic in situ hybridization" Mol. Brain Res.53. 1-12 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Louis, C.A.,et al: "Distinct arginase isoforms expressed in primary and transformed macrophages : regulation by oxygen tension" Am. J.Physiol.274. R775-R782 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, T.,et al: "Hypoglycemia-associated hyperammonemia caused by impaired expression of ornithine cycle enzyme genes in C/EBpx knockout mice" J.Biol.Chem.273. 27505-27510 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyanaka, K.,et al.: "Immunohistochemical Iocalization of arginase II and other enzymes of arginine metabolism in rat kidney and liver" Histochem. J.30. 741-751 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori, M.,et al.: "Regulation of the urea cycle enzyme genes in nitric oxide synthesis" J.Inher. metab. Dis. (Suppl. 1). 21. 59-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwase, K., et al.: "Precise distribution of neuronal nitric oxide synthase mRNA in the rat brain revealed by non-radioisotopic in situ hybridization" Mol.Brain Res. 53. 1-12 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Louis, C.A.et al.: "Distinct arginase isoforms expressed in primary and transformed macrophages : regulation by oxygen tension" Am.J.Physiol.274. R775-R782 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, T., et al.: "Hypoglycemia-associated hyperammonemia caused by impaired expression of ornithine cycle enzyme genes in C/EBPalpha knockout mice" J.Biol.Chem.273. 27505-27510 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyanaka, K., et al.: "Immunohistochemical localization of arginase II and other enzymes of arginine metabolism in rat kidney and liver" Histochem.J.30. 741-751 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, M., et al.: "Regulation of the urea cycle enzyme genes in nitric oxide synthesis" J.Inher.metab.Dis.(Suppl.1). 21. 59-71 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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