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1998 Fiscal Year Final Research Report Summary

Joint Study on Nature of Histone Variants by Gene Disruption

Research Project

Project/Area Number 09044327
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Structural biochemistry
Research InstitutionMiyazaki Medical College

Principal Investigator

NAKAYAMA Tatsuo  Miyazaki Medical College, Professor, 医学部, 教授 (60031712)

Co-Investigator(Kenkyū-buntansha) BUERSTEDDE J  ハンブルグ大学, 教授
TAKEDA Shunichi  Kyoto University, Faculty of Medicine, Professor, 大学院・医学研究科, 教授 (60188191)
KIKUCHI Hidehiko  Miyazaki Medical College, Assistant Professor, 医学部, 助手 (10301384)
TAKECHI Shinji  Miyazaki Medical College, Assistant Professor, 医学部, 助手 (10222100)
TAKAMI Yasunari  Miyazaki Medical College, Associate Professor, 医学部, 講師 (80236356)
JEAN-MARIE Buerstedde  Hamburg University, Professor
Project Period (FY) 1998
KeywordsGene targeting technique / DT40 / Histone variants / Chromatin structure / Transcription regulation / 2D-PAGE
Research Abstract

Thirty-nine of the 44 chicken H1 and core histone genes are located in a major histone gene cluster of 110 kb. In this study, using gene targeting techniques, we generated several DT4O mutants, respectively, which are devoid of one or two particular histone genes, one allele of the major gene cluster of 110 kb, an approximately 57 kb segment of the cluster carrying 21 genes, and 11 of the 12 H1 gene copies. Systematic analyses of the resultant mutants led us to some noticeable conclusions, as follows.
1)All of the histone gene families have the inherent ability to compensate for the deletion of approximately half of their own constituents, and to maintain the amount of each of the histone subtypes in stoichiometric balance, based on increases in the expression of the remaining genes, regardless of the complete disruption of one allele of the major gene cluster or the disruption of approximately half segments of the two alleles. Therefore, one allele of the major histone gene cluster is enough for cell proliferation.
2)The deletion of 11 of the 12 H1 gene copies does not affect cell functions, i.e. the growth rate and global chromatin structure. These results indicate that only one copy of the H1 genes is enough for cell proliferation.
3)The protein patterns are not altered in the case of the deletion of one allele of the cluster, due to no changes in the composition of any histone variant, but vary in the case of the deletion of approximately half segments of the cluster, and the latter plus more Hl gene(s), due to changes in the quality of the H1 and core variants.
4)The protein patterns are altered in the mutants, respectively, lacking particular Hl and H2B variants. The variable proteins are specific for the corresponding mutants, suggesting that the H1 and core variants should be individually involved in regulation of the expression of specific genes, through alterations in the chrornatin structure localized in specific genomic regions.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Takami, Y.: "An approximately half set of histone genes is enough for cell proliferation and a lack of several histone variants causes protein pattern changes in the DT40 chicken B cell line" J.Mol.Biol.265. 394-408 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takami, Y.: "One allele of the major histone gene cluster is enough for cell proliferation of the DT40 chicken B cell line" Biochim.Biophys.Acta. 1354. 105-115 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takami, Y.: "A single copy of linker Hl genes is enough for proliferation of the DT40 chicken B cell line, and linker Hl variants participate in regulation of gene expression" Genes to Cells. 2. 711-723 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama, T.: "Systematic analysis of the nature of histone genes in the chicken DT40 B cell line using gene targeting techniques" Trends in Comp.Biochem.Physiol.4. 211-217 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ahmad, A: "WD repeats of the p48 subunit of chicken chromatin assembly factor-1 required for in vitro interaction with chicken histone deacetylase-2" J.Biol.Chem.submitted.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takami, Y., Takeda, S.and Nakayama, T.: "An approximately half set of histone genes is enough for cell proliferation and a lack of several histone variants causes protein pattern changes in the DT40 chicken B cell line." J.Mol.Biol.265. 394-408 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takami, Y.and Nakayama, T.: "One allele of the major histone gene cluster is enough for cell proliferation of the DT40 chicken B cell line." Biochim.Biophys.Acta. 1354. 105-115 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takami, Y.and Nakayama, T.: "A single copy of linker H1 genes is enough for proliferation of the DT40 chicken B cell line, and linker H1 variants participate in regulation of gene expression." Genes to Cells. 2. 711-723 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, T.and Takami, Y.: "Systematic analysis of the nature of histone genes in the chicken DT40 B cell line using gene targeting techniques." Trends in Comp.Biochem.Physiol.4. 211-217 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ahmad, A., Takami, Y.and Nakayama, T.: "WD repeats of the p48 subunit of chicken chromatin assembly factor-1 required for in vitro interaction with chicken histone deacetylase-2." J.Biol.Chem.(submitted). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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