1997 Fiscal Year Annual Research Report

植物の感覚と運動に関する化学的研究

Research Project

Project/Area Number	09101001
Research Institution	Keio University
Principal Investigator	山村庄亮慶應義塾大学, 理工学部, 教授 (40076708)
Co-Investigator(Kenkyū-buntansha)	横谷香織筑波大学, 応用生物化学系, 助手 (10217531) 長谷川宏司筑波大学, 応用生物化学系, 教授 (70094167) 上田実慶應義塾大学, 理工学部, 助手 (60265931) 小瀬村誠治慶應義塾大学, 理工学部, 講師 (70231313)
Keywords	植物の光屈性 / 光誘起成長抑性物質 / オーキシン拮抗作用 / 植物の就眠運動 / 生物時計 / 植物の就眠及び覚醒物質 / サーカディアン・リズム
Research Abstract	植物の光屈性:光屈性についてはCholodony-Went説が定説となっているが、最近、光屈性の原因として光側に成長抑制物質が生成することが示された。筆者らは、ダイコン、トウモロコシなど数種の植物について光誘起成長抑制物質(光屈性天然分子)を明らかにしてきた。さらに、ヒマワリ、キャベツなどについてもそれぞれ異なった光屈性天然分子を見出し、その生成機構を明らかにした。一方、トウモロコシの光屈性天然分子については、成長を促進するオーキシンと拮抗することもわかり、その標的生体高分子(ABP1:auxin-binding protein)との相互作用を明らかにするために、光親和性標識化合物の合成に成功した。さらに、光屈性反応との関連性が示唆されているSAUR遺伝子をダイコンから単離し、そのプロモーター配列を含む全塩基配列を決定した。植物の就眠運動と生物時計:一般に、オジギソウやネムノキに代表される多くの植物は、ほぼ一日周期で葉の開閉運動(就眠運動)を行う。この運動は外部の刺激(光、温度など)により影響を受けるが、本質的には体内時計により制御される。従来、「就眠物質は植物に共通」と専門書に記載されているが、筆者らは定説に反して植物の数種によりそれぞれ異なった就眠物質を発見した。また、葉を開かせる物質(覚醒物質)をネムノキ、カワラケツメイ等から単離することに成功した。さらに、植物の就眠運動が就眠物質と覚醒物質のバランスに依存することもin vitro及びin vivoの実験で明らかにすることができた。特に、メドハギの場合、夕方になると、グリコシダーゼが働いて覚醒物質の濃度が減少することも実験的に分子レベルで証明した。

Research Products
(20 results)

All Other

All Publications (20 results)

[Publications] S.Kosemura: "Design of photoaffinity reagents for labeling the auxin receptor in maize." Tetrahedron Lett.38. 2125-2121 (1997)
[Publications] M.Ueda: "The chemical control of leaf-movement in a nyctinastic plant,Lespedeza cuneata G.Don." Tetrahedron Lett.38. 2497-2500 (1997)
[Publications] M.Ueda: "cis-p-Coumaroylagmatine,the genuine leaf-opening substance of a nyctinastic plant,Albizza julibrissin D." Tetrahedron Lett.38. 3253-3256 (1997)
[Publications] K.Miyamoto: "Inhibition of abscission of bean petiole explants by lepidimoide." J.Plant Growth Regul.16. 7-9 (1997)
[Publications] K.Miyamoto: "Inhibitory effect of lepidimoide on senescence in Avena leaf segments." J.Plant Physiol.150. 133-136 (1997)
[Publications] K.Yokotani-Tomita: "Light-induced auxin-inhibiting substance from sunflower(Helianthus annuus L.)seedlings." Phytochemistry. 46. 503-506 (1997)
[Publications] S.Kosemura: "Light-induced auxin-inhibiting substance from Cabbage(Brassica oleacea L.)Shoots." Tetrahedron Lett.38. 8327-8330 (1997)
[Publications] T.Anai: "Isolation and characterization of an auxin-binding protein gene from radish,and its expression in insect cells." Physiologica Plantarum. 101. 606-611 (1997)
[Publications] K.Yamada: "Exudation of an allelopathic substance lepidimoide from seeds during germination." Plant Growth Regulation. 22. 189-192 (1997)
[Publications] Y.Anai: "Comparison of abpl primary sequences from monocotyledonous and dicotyledonous species." J.Plant Physiol.151. 446-449 (1997)
[Publications] S.Kosemura: "Citreoazopyrone,a novel metabolite of a hybrid strain KO 0011 derived from Penicillum citreo-viride B.IFO 6200 and 4692." Tetrahedron Lett.38. 3025-3026 (1997)
[Publications] S.Kosemura: "Isolation and biosynthetic pathway for citreohybridones from the hybrid strain KO 0031 derived from Penicillum species." Tetrahedron Lett.38. 6221-6224 (1997)
[Publications] S.Kosemura: "Citreospirosteroid,a new metabolite of a hybrid strain KO 0011 derived from Penicillum citeo-viride B.IFO 6200 and 4692." Tetrahedron Lett.38. 7373-7374 (1997)
[Publications] M.Ueda: "The Enzymatic transformation of leaf-movement factor in Lespedeza cuneata G.Don controlled by a biological clock." Chemistry Lett.179-180 (1998)
[Publications] K.Yokotani-Tomita: "Growth-promoting allelopathic substance exuded from germinating Arabidopsis thaliana seeds" Phytochemistry. 47. 1-2 (1998)
[Publications] K.Yamada: "An allelopathic substance exuded from germinating watermelon seeds." Plant Growth Regulation. (in press). (1998)
[Publications] K.Yamada: "Lepidimoide promotes light-induced chlorophyll accumulation in cotyledons of sunflower seedlings." J.Plant Growth Regulation. (in press). (1997)
[Publications] T.Anai: "Nucleotide sequence of oat(Avena sativa L.)cDNA encoding an auxin-binding protein(ABP1)." DNA Sequence. (in press). (1997)
[Publications] T.Anai: "Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings." DNA sequence. (in press). (1998)
[Publications] M.Ueda: "Current Organic Chemistry" Chemical Studies on Plant Movement.(in press), (1998)

1997 Fiscal Year Annual Research Report

植物の感覚と運動に関する化学的研究

Principal Investigator

山村 庄亮 慶應義塾大学, 理工学部, 教授 (40076708)

Research Products

[Publications] S.Kosemura: "Design of photoaffinity reagents for labeling the auxin receptor in maize." Tetrahedron Lett.38. 2125-2121 (1997)

[Publications] M.Ueda: "The chemical control of leaf-movement in a nyctinastic plant,Lespedeza cuneata G.Don." Tetrahedron Lett.38. 2497-2500 (1997)

[Publications] M.Ueda: "cis-p-Coumaroylagmatine,the genuine leaf-opening substance of a nyctinastic plant,Albizza julibrissin D." Tetrahedron Lett.38. 3253-3256 (1997)

[Publications] K.Miyamoto: "Inhibition of abscission of bean petiole explants by lepidimoide." J.Plant Growth Regul.16. 7-9 (1997)

[Publications] K.Miyamoto: "Inhibitory effect of lepidimoide on senescence in Avena leaf segments." J.Plant Physiol.150. 133-136 (1997)

[Publications] K.Yokotani-Tomita: "Light-induced auxin-inhibiting substance from sunflower(Helianthus annuus L.)seedlings." Phytochemistry. 46. 503-506 (1997)

[Publications] S.Kosemura: "Light-induced auxin-inhibiting substance from Cabbage(Brassica oleacea L.)Shoots." Tetrahedron Lett.38. 8327-8330 (1997)

[Publications] T.Anai: "Isolation and characterization of an auxin-binding protein gene from radish,and its expression in insect cells." Physiologica Plantarum. 101. 606-611 (1997)

[Publications] K.Yamada: "Exudation of an allelopathic substance lepidimoide from seeds during germination." Plant Growth Regulation. 22. 189-192 (1997)

[Publications] Y.Anai: "Comparison of abpl primary sequences from monocotyledonous and dicotyledonous species." J.Plant Physiol.151. 446-449 (1997)

[Publications] S.Kosemura: "Citreoazopyrone,a novel metabolite of a hybrid strain KO 0011 derived from Penicillum citreo-viride B.IFO 6200 and 4692." Tetrahedron Lett.38. 3025-3026 (1997)

[Publications] S.Kosemura: "Isolation and biosynthetic pathway for citreohybridones from the hybrid strain KO 0031 derived from Penicillum species." Tetrahedron Lett.38. 6221-6224 (1997)

[Publications] S.Kosemura: "Citreospirosteroid,a new metabolite of a hybrid strain KO 0011 derived from Penicillum citeo-viride B.IFO 6200 and 4692." Tetrahedron Lett.38. 7373-7374 (1997)

[Publications] M.Ueda: "The Enzymatic transformation of leaf-movement factor in Lespedeza cuneata G.Don controlled by a biological clock." Chemistry Lett.179-180 (1998)

[Publications] K.Yokotani-Tomita: "Growth-promoting allelopathic substance exuded from germinating Arabidopsis thaliana seeds" Phytochemistry. 47. 1-2 (1998)

[Publications] K.Yamada: "An allelopathic substance exuded from germinating watermelon seeds." Plant Growth Regulation. (in press). (1998)

[Publications] K.Yamada: "Lepidimoide promotes light-induced chlorophyll accumulation in cotyledons of sunflower seedlings." J.Plant Growth Regulation. (in press). (1997)

[Publications] T.Anai: "Nucleotide sequence of oat(Avena sativa L.)cDNA encoding an auxin-binding protein(ABP1)." DNA Sequence. (in press). (1997)

[Publications] T.Anai: "Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings." DNA sequence. (in press). (1998)

[Publications] M.Ueda: "Current Organic Chemistry" Chemical Studies on Plant Movement.(in press), (1998)

山村庄亮慶應義塾大学, 理工学部, 教授 (40076708)