1997 Fiscal Year Annual Research Report

ヒトリンパ造血前駆細胞の純化とその増幅法の開発

Research Project

Project/Area Number	09250202
Research Institution	The University of Tokyo
Principal Investigator	辻浩一郎東京大学, 医科学研究所, 助教授 (50179991)
Keywords	ヒトB細胞 / マウスストローマ細胞 / SCF / G‐CSF
Research Abstract	1.マウスストローマ細胞株MS‐5を用いたヒトCD34+細胞からのB細胞誘導系の確立我々は、ヒト臍帯血CD34+細胞をマウス骨髄細胞由来ストローマ細胞株、MS‐5と2‐4週間共培養すると、ヒトCD19+proB細胞が誘導されることを見出した。これらのCD19+細胞の88%がCD10、45%がCD20を発現していたが、Cμは認められなかった。我々が確立した培養法は、ヒトB細胞の初期分化の解析に有用であると考えられた。また、このB細胞誘導系においてはMS‐5細胞との接着は必須であり、MS‐5細胞上にはヒトB細胞の初期分化に関与する未知の膜結合性分子が発現されていると考えられ、その同定はヒトB細胞の分化を解析するうえで、極めて重要と考えられた。 2.種々のサイトカインのヒトB細胞の初期分化に及ぼす影響の検討我々が確立した上述の培養系にinterleukin(IL)‐3、IL‐6、granulocyte‐macrophage colony‐stimulating factor(GM‐CSF)、、stem cell factor(SCF)、granulocyte colony‐stimulating factor(G‐CSF)を添加し、それぞれのサイトカインのB細胞誘導に及ぼす影響について検討した。IL‐3、IL‐6、GM‐CSFはB細胞誘導にほとんど影響を及ぼさなかったが、SCF、G‐CSF添加により誘導されるB細胞数は増加した。特にSCF、G‐CSF両者存在下では、誘導されるproB細胞は著増し、培養6週での解析ではその一部にはCμ、Sμの発現も認められた。この結果より、SCF、G‐CSFはヒトB細胞の分化に関与していると考えられた。

Research Products

(29 results)

All Other

All Publications (29 results)

[Publications] Akasaka T: "The rele of mel‐18,a mammalian homelog of Drosophia Polycomb groupgene,during the IL-7-dependent proliferation of lymphocyte precursors" Immunity. 7. 135-146 (1997)
[Publications] Nishijima I: "Hemopoietic and lymphopoietic responses in human GM-CSF receptor transgenic mice injected with human GM-CSF" Blood. 90. 1031-1038 (1997)
[Publications] Umemoto Y: "Leptin stimulates the proliferation of murine myelocytic and primitive hematopoietic progenitor cells" Blood. 90. 3438-3443 (1997)
[Publications] Ebihara Y: "Synergistic action of Fl+3 and gp130 signalings in human hematopoiesis" Blood. 90. 4363-4368 (1997)
[Publications] Yoshida M: "Thrombopoietin alone stimulates the early proliferation and survival of human erythroid,myeloid and multipotential progenitors in serum-free culture" Br J.Haematol. 98. 254-264 (1997)
[Publications] Muraoka K: "Thrombopoietin-Independent effect of interferon8 on the proliferation of human megakaryocyte progenitors" Br.J.Haematol. 98. 265-273 (1997)
[Publications] Tsuji K: "Rde of sduble interleukin-6 recepter with interleukin-6 in human hetnatopoiesis" Hematology. 2. 143-149 (1997)
[Publications] Eguchi M: "Morphological and cytological changes in human mast cells during culture" Med.Electron Microsc. 30. 25-30 (1997)
[Publications] Mukouyama Y: "Induction of hematopoiesis by oncostatin M in the AGM region" Immunity. (in press).
[Publications] Sui X: "Soluble IL-6 receptor with IL-6 stimulates megakaryo poiesis from human CD34+ cells through gp130 signaling" Blood. (in press).
[Publications] Takahashi T: "A potential molecular approach to exviro expansion with recembinant EGFR-expressing adenovirus vector" Blood. (in press).
[Publications] Nishihara M: "A combination of stem cell factor and granulocyte colony-stimulating factor enhances proliferation and differentiation of human progenitor B cells supported by murine stromal cellline MS-5" Eur.J.Immunal. (in press).
[Publications] Tsuji K: "Leptin,the obese ptoduct and hematopoiesis" Leukemia & Lymphoma. (in press).
[Publications] Tsuji K: "Interferon-γ and human megakaryocyte progenitcrs" Leukemia & Lymphoma. (in press).
[Publications] Yang F: "Hematopoiesis in human G-CSF receptor-transgenic mouse" Int.J.Hematol. (in press).
[Publications] Maruyama K: "Characterization of peripheral blood progenitor cells moloilized by nartograstim (N-terminal replaced granulocyte colony-stimulating factor)in ncrmal volunteers" Bone Marrow Transplant. (in press).
[Publications] Nakahata T: "Exvivo expansion of human primitive hemopoietic progenitors" Stem Cells. (in press).
[Publications] 辻浩一郎: "造血幹細胞の増幅と幹細胞移植" 細胞. 29. 138-141 (1997)
[Publications] 辻浩一郎: "造血幹細胞の増幅と幹細胞移植" 細胞. 29. 138-141 (1997)
[Publications] 辻浩一郎: "マスト細胞と造血幹細胞" アレルギーの領域. 4. 60-65 (1997)
[Publications] 辻浩一郎: "造血幹細胞の性状" 炎症と免疫. 5. 377-383 (1997)
[Publications] 辻浩一郎: "血液細胞の分化とサイトカイン" Mebio. 14・4. 49-57 (1997)
[Publications] 海老原康博: "寛解導入療法中にall-trono vetiroic acid(ATRA)による頭蓋内圧亢進症状(psendotumor cerebri)を呈した急性前骨髄性白血症(APL)の1男児例" 日小血会誌. 11. 445-449 (1997)
[Publications] 辻浩一郎: "AGM(aorta‐gonad-mesonephros)領域と造血幹細胞" 臨床免疫. (印刷中).
[Publications] 辻浩一郎: "造血幹細胞の性状(LTC-IC、CFU-S、CD34細胞など)" 医学のあゆみ. (印刷中).
[Publications] 浅野茂隆: "疾病とサイトカイン-病因から治療への応用まで-" 真興交易医書, 271 (1997)
[Publications] 白木和夫: "小児科学" 医学書院, 1642 (1997)
[Publications] 浅野茂隆: "血液内科学" 中外医学社(印刷中),
[Publications] 黒川清: "内科学教科書" 文光堂(印刷中),

1997 Fiscal Year Annual Research Report

ヒトリンパ造血前駆細胞の純化とその増幅法の開発

Principal Investigator

辻 浩一郎 東京大学, 医科学研究所, 助教授 (50179991)

Research Products

[Publications] Akasaka T: "The rele of mel‐18,a mammalian homelog of Drosophia Polycomb groupgene,during the IL-7-dependent proliferation of lymphocyte precursors" Immunity. 7. 135-146 (1997)

[Publications] Nishijima I: "Hemopoietic and lymphopoietic responses in human GM-CSF receptor transgenic mice injected with human GM-CSF" Blood. 90. 1031-1038 (1997)

[Publications] Umemoto Y: "Leptin stimulates the proliferation of murine myelocytic and primitive hematopoietic progenitor cells" Blood. 90. 3438-3443 (1997)

[Publications] Ebihara Y: "Synergistic action of Fl+3 and gp130 signalings in human hematopoiesis" Blood. 90. 4363-4368 (1997)

[Publications] Yoshida M: "Thrombopoietin alone stimulates the early proliferation and survival of human erythroid,myeloid and multipotential progenitors in serum-free culture" Br J.Haematol. 98. 254-264 (1997)

[Publications] Muraoka K: "Thrombopoietin-Independent effect of interferon8 on the proliferation of human megakaryocyte progenitors" Br.J.Haematol. 98. 265-273 (1997)

[Publications] Tsuji K: "Rde of sduble interleukin-6 recepter with interleukin-6 in human hetnatopoiesis" Hematology. 2. 143-149 (1997)

[Publications] Eguchi M: "Morphological and cytological changes in human mast cells during culture" Med.Electron Microsc. 30. 25-30 (1997)

[Publications] Mukouyama Y: "Induction of hematopoiesis by oncostatin M in the AGM region" Immunity. (in press).

[Publications] Sui X: "Soluble IL-6 receptor with IL-6 stimulates megakaryo poiesis from human CD34+ cells through gp130 signaling" Blood. (in press).

[Publications] Takahashi T: "A potential molecular approach to exviro expansion with recembinant EGFR-expressing adenovirus vector" Blood. (in press).

[Publications] Nishihara M: "A combination of stem cell factor and granulocyte colony-stimulating factor enhances proliferation and differentiation of human progenitor B cells supported by murine stromal cellline MS-5" Eur.J.Immunal. (in press).

[Publications] Tsuji K: "Leptin,the obese ptoduct and hematopoiesis" Leukemia & Lymphoma. (in press).

[Publications] Tsuji K: "Interferon-γ and human megakaryocyte progenitcrs" Leukemia & Lymphoma. (in press).

[Publications] Yang F: "Hematopoiesis in human G-CSF receptor-transgenic mouse" Int.J.Hematol. (in press).

[Publications] Maruyama K: "Characterization of peripheral blood progenitor cells moloilized by nartograstim (N-terminal replaced granulocyte colony-stimulating factor)in ncrmal volunteers" Bone Marrow Transplant. (in press).

[Publications] Nakahata T: "Exvivo expansion of human primitive hemopoietic progenitors" Stem Cells. (in press).

[Publications] 辻浩一郎: "造血幹細胞の増幅と幹細胞移植" 細胞. 29. 138-141 (1997)

[Publications] 辻浩一郎: "造血幹細胞の増幅と幹細胞移植" 細胞. 29. 138-141 (1997)

[Publications] 辻浩一郎: "マスト細胞と造血幹細胞" アレルギーの領域. 4. 60-65 (1997)

[Publications] 辻浩一郎: "造血幹細胞の性状" 炎症と免疫. 5. 377-383 (1997)

[Publications] 辻浩一郎: "血液細胞の分化とサイトカイン" Mebio. 14・4. 49-57 (1997)

[Publications] 海老原康博: "寛解導入療法中にall-trono vetiroic acid(ATRA)による頭蓋内圧亢進症状(psendotumor cerebri)を呈した急性前骨髄性白血症(APL)の1男児例" 日小血会誌. 11. 445-449 (1997)

[Publications] 辻浩一郎: "AGM(aorta‐gonad-mesonephros)領域と造血幹細胞" 臨床免疫. (印刷中).

[Publications] 辻浩一郎: "造血幹細胞の性状(LTC-IC、CFU-S、CD34細胞など)" 医学のあゆみ. (印刷中).

[Publications] 浅野茂隆: "疾病とサイトカイン-病因から治療への応用まで-" 真興交易医書, 271 (1997)

[Publications] 白木和夫: "小児科学" 医学書院, 1642 (1997)

[Publications] 浅野茂隆: "血液内科学" 中外医学社(印刷中),

[Publications] 黒川清: "内科学教科書" 文光堂(印刷中),

辻浩一郎東京大学, 医科学研究所, 助教授 (50179991)