1997 Fiscal Year Annual Research Report

心筋及び平滑筋のイオンチャネルとイオン駆動型トランスポータ

Research Project

Project/Area Number	09257208
Research Institution	The University of Tokyo
Principal Investigator	中島敏明東京大学, 医学部・付属病院, 助手 (50227790)
Co-Investigator(Kenkyū-buntansha)	岩沢邦明東京大学, 医学部・付属病院, 医員狭間比左徳東京大学, 医学部・付属病院, 医員
Keywords	vascular smooth muscle / vasopressin / fatty acids / ionic currents / endothelin-1 / pH / Ca^<2+>-permeable channels / eicosapentaenoic acid
Research Abstract	大動脈平滑筋細胞においてendothelin-1,vasopressinなどの血管収縮agonistは、細胞内Ca^<2+>を増加させる。このCa^<2+>上昇は、細胞内からのCa^<2+>放出と、細胞外からのCa^<2+>流入が関与している。細胞外pHは、この細胞外からのCa^<2+>流入に強く影響すること、これにはCa^<2+>透過性非選択性陽イオンチャネル及びCa^<2+>-release aotivated Ca^<2+>-influx(CRAC)に対する作用によると考えられた(J.Physiol 1997,503,2)。更に、eicosapentaenoic acid(EPA)などのω-3系多価不飽和脂肪酸は、このagonistによるCa^<2+>透過性非選択性陽イオンチャネルを抑制すると共に、EPA慢性処理細胞では、細胞膜EPA含量が増加し、agonistによるCa^<2+>上昇が著明に抑制されることを報告した。

Research Products

(9 results)

All Other

All Publications (9 results)

[Publications] Nakajima T et al.: "Effects of pirmenol on action potential and membrane currents in atrial myocytes" European Journal of Pharmacology. 344. 287-297 (1998)
[Publications] Asano M et al.: "Influence of cellular incorporation of n-3 eicosapentaenoic acid on intracellular Ca^<2+> concentration and membrane potential in vascular smooth muscle cells" Atherosclerosis. 138. 117-127 (1998)
[Publications] Iwasawa K et al.: "Effects of extracellular pH on receptor-mediated Ca^<2+>-influx in A7r5 rat smooth muscle cells: involvement of two different types of channels" Journal of Physiology. 503. 237-251 (1997)
[Publications] Mizutani M et al.: "ω-3 polyunsaturated fatty acids inhibit migration of human vascula smooth muscle cells in vitro." Life Sciences. 61. 269-274 (1997)
[Publications] Hamada E et al.: "Effect of caffeine on mucus secretion and agonist-dependent Ca^<2+> mobilization in human gastric mucus secreting cells" Biochimica et Biophysica ACTA. 1356. 198-206 (1997)
[Publications] Asano M et al.: "Inhibitory effects of ω-3 polyunsaturated fatty acids on receptor-mediated non-selective cation currents in rat A7r5 vascular smooth muscle cells" British Journal of Pharmacology. 120. 1367-1375 (1997)
[Publications] Nakajima T et al.: "Extracellular Mg^<2+> inhibits receptor-mediated Ca^<2+>-permeable non-selective cation currents in aortic smooth muscle cells." European Journal of Pharmacology. 320. 81-86 (1997)
[Publications] Okuda Y et al.: "Eicosapentaenoic acid enhances nitri oxide production by cultured human endothelial cells." Biochemical and Biophysical Research Communications. 232. 487-491 (1997)
[Publications] Asakura Y et al.: "Effects of benedipine hydrochloride on DNA synthesis and cytosolic free Ca^<2+> concentrations of rat aortic smooth muscle cells." Diabetic Research. 32. 237-244 (1997)

1997 Fiscal Year Annual Research Report

心筋及び平滑筋のイオンチャネルとイオン駆動型トランスポータ

Principal Investigator

中島 敏明 東京大学, 医学部・付属病院, 助手 (50227790)

Research Products

[Publications] Nakajima T et al.: "Effects of pirmenol on action potential and membrane currents in atrial myocytes" European Journal of Pharmacology. 344. 287-297 (1998)

[Publications] Asano M et al.: "Influence of cellular incorporation of n-3 eicosapentaenoic acid on intracellular Ca^<2+> concentration and membrane potential in vascular smooth muscle cells" Atherosclerosis. 138. 117-127 (1998)

[Publications] Iwasawa K et al.: "Effects of extracellular pH on receptor-mediated Ca^<2+>-influx in A7r5 rat smooth muscle cells: involvement of two different types of channels" Journal of Physiology. 503. 237-251 (1997)

[Publications] Mizutani M et al.: "ω-3 polyunsaturated fatty acids inhibit migration of human vascula smooth muscle cells in vitro." Life Sciences. 61. 269-274 (1997)

[Publications] Hamada E et al.: "Effect of caffeine on mucus secretion and agonist-dependent Ca^<2+> mobilization in human gastric mucus secreting cells" Biochimica et Biophysica ACTA. 1356. 198-206 (1997)

[Publications] Asano M et al.: "Inhibitory effects of ω-3 polyunsaturated fatty acids on receptor-mediated non-selective cation currents in rat A7r5 vascular smooth muscle cells" British Journal of Pharmacology. 120. 1367-1375 (1997)

[Publications] Nakajima T et al.: "Extracellular Mg^<2+> inhibits receptor-mediated Ca^<2+>-permeable non-selective cation currents in aortic smooth muscle cells." European Journal of Pharmacology. 320. 81-86 (1997)

[Publications] Okuda Y et al.: "Eicosapentaenoic acid enhances nitri oxide production by cultured human endothelial cells." Biochemical and Biophysical Research Communications. 232. 487-491 (1997)

[Publications] Asakura Y et al.: "Effects of benedipine hydrochloride on DNA synthesis and cytosolic free Ca^<2+> concentrations of rat aortic smooth muscle cells." Diabetic Research. 32. 237-244 (1997)

中島敏明東京大学, 医学部・付属病院, 助手 (50227790)