1998 Fiscal Year Annual Research Report

光合成機能統御の細胞内コミュニケーション機構

Research Project

Project/Area Number	09274103
Research Institution	Kyoto University
Principal Investigator	佐藤文彦京都大学, 農学研究科, 教授 (10127087)
Co-Investigator(Kenkyū-buntansha)	西村幹夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (80093061) 長谷俊治大阪大学, 蛋白質研究所, 教授 (00127276) 小俣達男名古屋大学, 生命農学研究科, 教授 (50175270) 杉田護名古屋大学, 人間情報学研究科, 教授 (70154474)
Keywords	葉緑体の遺伝子発現制御 / DNA結合タンパク質 / 葉緑体形質転換 / 窒素同化と炭素同化のクロストーク / 電子分配 / マイクロボディの機能転換 / 形質転換植物 / 遺伝子破壊
Research Abstract	1) 佐藤は葉緑体DNA結合蛋白質(CND41)が弱酸性条件においてRubisco大サブユニットを部位特異的に切断すること等、CND41のprotease活性を詳細に解明した。さらに、培養細胞、植物体における時期・組織特異的発現、糖による誘導等を明らかとするとともに、ゲノム遺伝子の単離と構造解析を行なった。また、アンチセンス形質転換体を用いてCND41が植物体の形態形成にも重要な役割を果たすことを示唆した。 2) 杉田はタバコ葉緑体RNA結合蛋白質の葉緑体内の存在量、存在形態、結合RNA分子種の特定等を検討し、葉緑体RNA結合蛋白質がストロマ中のRNAの安定性に関与していることを示唆した。また、核コードのファージ型RNAポリメラーゼ(RpoZ)ホモログをN.sylvestrisより単離し、その構造を決定した。 3) 小俣はラン藻の硝酸同化オペロンの転写制御における「基質による正の制御」と「窒素同化産物による負の制御」の相互関係を明らかとした。また、硝酸能動輸送体(NRT)のRドメインとPII蛋白質がそれぞれNRTの活性を抑制すること、およびNRTとRドメインを介する経路以外に炭酸同化の阻害に応答してNRTを制御する機構を見いだした。 4) 長谷はFdを欠失したラン藻にトウモロコシの葉肉細胞特異的FdIと維管束鞘細胞特異的FdIIを導入し、FdIとFdIIの炭素と窒素同化系への電子分配の効率が異なる可能性を明らかとした。一方、光合成器官と非光合成器官における還元力の利用特性を決定する要因として、FdとFd-NADP^+還元酵素の組み合わせが重要であることを明らかとした。 5) 西村はマイクロボディ酵素の発現制御に関わるalternative splicingを解析し、生じた蛋白質の〔末端の移行シグナルの有無により細胞内局在が制御されていることを明らかとした。また、マイクロボデイ酵素cDNAクローニング、その構造解析、これら蛋白質の強制発現形質転換体を用いて、これら蛋白質がマイクロボディの機能転換、さらには植物体機能において果たす役割を明らかとした。

Research Products

(30 results)

All Other

All Publications (30 results)

[Publications] Kitajima,S.: "Constitutive expression of the neutral PR-5 (OLP,PR-5d) gene in roots and cultured cells of tobacco is mediated by ethylene-responsive cis-element AGCCGCC sequences." Plant Cell Reports. 18. 173-179 (1998)
[Publications] Endo,T.: "Inhibition of photosystem II of spinach by lichen-derived depsides." Biosci.Biotech.Biochem.62. 2023-2027 (1998)
[Publications] Endo,T: "NAD(P)H dehydrogenase-dependent, antimycin a-sensitive electron donation to plastoquinone in tobacco chloroplasts." Plant Cell Physiol.39. 1226-1231 (1998)
[Publications] Kitajima,S.: "Plant pathogeniesis-related proteins: molecular mechanisms of gene expression and protein function." J.Biochem.125. 1-8 (1991)
[Publications] Ifuku,K.: "Reconstitution of photosynthetic oxygen evolving activity with heterologous OEC23 protein expressed in E.coli." Research in Photosynthesis, Kluwer Acad.Pub. (in press).
[Publications] Murakami,S: "Purification of CND41: chloroplast nucleoid DNA-binding protein with proteolytic activity." Research in Photosynthesis, Kluwer Acad.Pub. (in press).
[Publications] Miyagi,T.: "Transcript analysis of tobacco plastid rps2/atp1/H/F/A operon reveals the existence of a non-consensus type II (NCII) promoter upstream to atpI coding sequence." Mol.Gen.Genet. 257. 299-307 (1998)
[Publications] Wakasugi,T: "Update gene map of tobacco chloroplast DNA" Plant Mol.Biol.Rep.16. 231-241 (1998)
[Publications] Sugiura,M: "Evolution and mechanism of translation in chloroplasts" Annu.Rev.Genet.32. 437-459 (1998)
[Publications] Fujita,Y: "Cloning of the gene encoding a protochlorophyllide reductase: the physiological significance of the coexistence of light-dependent and-independent protochlorophyllide reduction systems in the cyanobacterium Plectoncrn boryanum." Plant Cell Physiol.39. 177-185 (1998)
[Publications] Yonekura-Sakakibara,K: "Molecular characterization of tobacco sulfite reductase:enzyme purification, gene cloning, and gene expression analysis." J.Biochem.124. 615-621 (1998)
[Publications] Okuhara,H: "Fd-GOGAT is necessary for the integration of photosynthetic C- and N- assimilation in cyanobacerium." Proc.XI International Photosynthesis Congress. (in press).
[Publications] Matsumura,T: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves." Plant Physiol.199. 481-488 (1999)
[Publications] Maeda,S: "cis-Acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechocystis sp.strain PCC 7942" J.Bacteriol.180. 4080-4088 (1998)
[Publications] Rodriguez,R: "Independence of carbon and nitrogen control in the posttranslational regulation of nitrate transport in the cyanobacterium Synechococcus sp.strain PCC 7942." FEBS Lett.432. 207-212 (1998)
[Publications] Maeda,S: "Nitrite-specific active transport system of the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.180. 6761-6763 (1998)
[Publications] Kato,A: "Glyoxysomal malate dehydrogenase in pumpkin: cloning of a cDNA and functional analysis of its presequence." Plant Cell Physiol.39. 186-195 (1998)
[Publications] Hayashi,M.: "2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects on glyoxysomal b-oxidation." Plant Cell. 10. 183-195 (1998)
[Publications] Hara-Nishimura,I: "Vacuolar processing enzymes in protein-storage vacuoles and lytic vacuoles" J.Plant Physiol.152. 668-674 (1998)
[Publications] Hayashi,M: "Functional transformation of plant peroxisomes." Cell Biochem.Biophys.(in press).
[Publications] Hayashi,H: "Molecular characerization of a glyoxysomal long-chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin." J.Biol.Chem.273. 8301-8307 (1998)
[Publications] Hara-Nishimura,I: "Transport of storage proteins to protein-strage vacuoles is mediated by large precursor-accumulating vesicles." Plant Cell. 10. 825-836 (1998)
[Publications] Yamada,K: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme." J.Biol.Chem.(in press).
[Publications] Ali,K.: "Levels of nonstructural carbohydrate in leaves and roots and some characteristics of chloroplasts after application of different amounts of nitrogen fertilizer to peach seedlings." J.Jap.Soc.Hort.Sci. (in press).
[Publications] Hayashi,M: "Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase." Plant Cell Physiol.(in press).
[Publications] Mano,S: "Light regulates alternative splicing of hydroxypyruvate reductase in pumpkin" Plant J.(in press).
[Publications] Koumoto,Y: "Chloroplast Cpn20 forms a tertrameric structure in Arabidopsis thaliana" Plant J.(in press).
[Publications] Nishimura,M: "Molecular chaperones and temperature stress" Stress Responses of Photosynthesis Organisms. (Elsevier). 83-92 (1998)
[Publications] Nishimura,M: "Microbody defective mutants of Arabidopsis" J.Plant Res.111. 329-332 (1998)
[Publications] Kato,A: "Transport peroxidomal prteins that are synthesized as large precurosors in plants." Cell Biochem.Biophy.(in press).

1998 Fiscal Year Annual Research Report

光合成機能統御の細胞内コミュニケーション機構

Principal Investigator

佐藤 文彦 京都大学, 農学研究科, 教授 (10127087)

Research Products

[Publications] Kitajima,S.: "Constitutive expression of the neutral PR-5 (OLP,PR-5d) gene in roots and cultured cells of tobacco is mediated by ethylene-responsive cis-element AGCCGCC sequences." Plant Cell Reports. 18. 173-179 (1998)

[Publications] Endo,T.: "Inhibition of photosystem II of spinach by lichen-derived depsides." Biosci.Biotech.Biochem.62. 2023-2027 (1998)

[Publications] Endo,T: "NAD(P)H dehydrogenase-dependent, antimycin a-sensitive electron donation to plastoquinone in tobacco chloroplasts." Plant Cell Physiol.39. 1226-1231 (1998)

[Publications] Kitajima,S.: "Plant pathogeniesis-related proteins: molecular mechanisms of gene expression and protein function." J.Biochem.125. 1-8 (1991)

[Publications] Ifuku,K.: "Reconstitution of photosynthetic oxygen evolving activity with heterologous OEC23 protein expressed in E.coli." Research in Photosynthesis, Kluwer Acad.Pub. (in press).

[Publications] Murakami,S: "Purification of CND41: chloroplast nucleoid DNA-binding protein with proteolytic activity." Research in Photosynthesis, Kluwer Acad.Pub. (in press).

[Publications] Miyagi,T.: "Transcript analysis of tobacco plastid rps2/atp1/H/F/A operon reveals the existence of a non-consensus type II (NCII) promoter upstream to atpI coding sequence." Mol.Gen.Genet. 257. 299-307 (1998)

[Publications] Wakasugi,T: "Update gene map of tobacco chloroplast DNA" Plant Mol.Biol.Rep.16. 231-241 (1998)

[Publications] Sugiura,M: "Evolution and mechanism of translation in chloroplasts" Annu.Rev.Genet.32. 437-459 (1998)

[Publications] Fujita,Y: "Cloning of the gene encoding a protochlorophyllide reductase: the physiological significance of the coexistence of light-dependent and-independent protochlorophyllide reduction systems in the cyanobacterium Plectoncrn boryanum." Plant Cell Physiol.39. 177-185 (1998)

[Publications] Yonekura-Sakakibara,K: "Molecular characterization of tobacco sulfite reductase:enzyme purification, gene cloning, and gene expression analysis." J.Biochem.124. 615-621 (1998)

[Publications] Okuhara,H: "Fd-GOGAT is necessary for the integration of photosynthetic C- and N- assimilation in cyanobacerium." Proc.XI International Photosynthesis Congress. (in press).

[Publications] Matsumura,T: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves." Plant Physiol.199. 481-488 (1999)

[Publications] Maeda,S: "cis-Acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechocystis sp.strain PCC 7942" J.Bacteriol.180. 4080-4088 (1998)

[Publications] Rodriguez,R: "Independence of carbon and nitrogen control in the posttranslational regulation of nitrate transport in the cyanobacterium Synechococcus sp.strain PCC 7942." FEBS Lett.432. 207-212 (1998)

[Publications] Maeda,S: "Nitrite-specific active transport system of the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.180. 6761-6763 (1998)

[Publications] Kato,A: "Glyoxysomal malate dehydrogenase in pumpkin: cloning of a cDNA and functional analysis of its presequence." Plant Cell Physiol.39. 186-195 (1998)

[Publications] Hayashi,M.: "2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects on glyoxysomal b-oxidation." Plant Cell. 10. 183-195 (1998)

[Publications] Hara-Nishimura,I: "Vacuolar processing enzymes in protein-storage vacuoles and lytic vacuoles" J.Plant Physiol.152. 668-674 (1998)

[Publications] Hayashi,M: "Functional transformation of plant peroxisomes." Cell Biochem.Biophys.(in press).

[Publications] Hayashi,H: "Molecular characerization of a glyoxysomal long-chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin." J.Biol.Chem.273. 8301-8307 (1998)

[Publications] Hara-Nishimura,I: "Transport of storage proteins to protein-strage vacuoles is mediated by large precursor-accumulating vesicles." Plant Cell. 10. 825-836 (1998)

[Publications] Yamada,K: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme." J.Biol.Chem.(in press).

[Publications] Ali,K.: "Levels of nonstructural carbohydrate in leaves and roots and some characteristics of chloroplasts after application of different amounts of nitrogen fertilizer to peach seedlings." J.Jap.Soc.Hort.Sci. (in press).

[Publications] Hayashi,M: "Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase." Plant Cell Physiol.(in press).

[Publications] Mano,S: "Light regulates alternative splicing of hydroxypyruvate reductase in pumpkin" Plant J.(in press).

[Publications] Koumoto,Y: "Chloroplast Cpn20 forms a tertrameric structure in Arabidopsis thaliana" Plant J.(in press).

[Publications] Nishimura,M: "Molecular chaperones and temperature stress" Stress Responses of Photosynthesis Organisms. (Elsevier). 83-92 (1998)

[Publications] Nishimura,M: "Microbody defective mutants of Arabidopsis" J.Plant Res.111. 329-332 (1998)

[Publications] Kato,A: "Transport peroxidomal prteins that are synthesized as large precurosors in plants." Cell Biochem.Biophy.(in press).

佐藤文彦京都大学, 農学研究科, 教授 (10127087)