1999 Fiscal Year Annual Research Report

光合成機能統御の細胞内コミュニケーション機構

Research Project

Project/Area Number	09274103
Research Institution	Kyoto University
Principal Investigator	佐藤文彦京都大学, 生命科学研究科, 教授 (10127087)
Co-Investigator(Kenkyū-buntansha)	長谷俊治大阪大学, 蛋白質研究所, 教授 (00127276) 小俣達男名古屋大学, 生命農学研究科, 教授 (50175270) 杉田護名古屋大学, 人間情報学研究科, 教授 (70154474) 西村幹夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (80093061)
Keywords	葉緑体の遺伝子発現制御 / DNA結合タンパク質 / 葉緑体形質転換 / 窒素同化と炭素同化のクロストーク / 電子分配 / マイクロボディの機能転換 / 形質転換植物 / 遺伝子破壊
Research Abstract	1)佐藤は生理的pHにおける葉緑体DNA結合タンパク質(CND41)のプロテアーゼ活性ついて検討を行ない、変性処理したRubiscoがCND41依存的に分解されること、一方、Rubiscoの活性化、あるいは、DNAの添加によりタンパク質分解が抑制されること等を認めた.さらに、形質転換植物体を用いてCND41が老化時のタンパク質分解に関与していることを示唆する結果を得、現在検討を進めている 2)杉田は葉緑体RNA結合蛋白質が種々のストロマRNAに結合し、RNAの安定性に関与していることを示唆するデータを得た。また、葉緑体遺伝子の転写に働くファージ型RNAポリメラーゼと推定されるRpoT-Cを含む3種のRpoTホモログ(RpoT-A〜C)をN.sylvestrisから単離した。 3)小俣はラン藻の炭素欠乏誘導性オペロンのひとつであるcmpABCDオペロンがHCO_3^-能動輸送体をコードしていることを証明し、このオペロンと他の炭素欠乏応答性遺伝子群の活性化には異なる転写因子が関与すること、また、炭素および窒素に応答して制御されるccmKLMNOオペロンには、炭素、窒素それぞれに応答するcis制御領域があることを明らかにした。さらに、PII蛋白質のリン酸化状態の変化とラン藻の硝酸同化の制御の間には直接の関連がないことを証明した。 4)長谷は葉肉細胞特異的なFdIと維管束鞘細胞特異的なFdIIがPSI周辺の電子の分配を制御することを示した。また、Fd-NADP^+還元酵素(FNR)と亜硫酸還元酵素(SiR)を用いて、植物根に存在するNADPH/FNR/Fdの電子伝達カスケードが非光合成器官におけるFd依存性の代謝反応の還元力供給系として働くこと、及びFdとの蛋白質間相互作用の構造特性を明らかにした。 5)西村はhydroxypyruvate reductaseと葉緑体に局在するascorbate peroxidaseがalternative splicingによって2種類の蛋白質として合成されることを明らかにしている。さらに、このalternative splicingが光により制御を受けていることを初めて明らかにするとともに、splicingのトランス因子であるSRタンパク質のcDNAクローニング及びその発現調節を解析した。

Research Products
(28 results)

All Other

All Publications (28 results)

[Publications] Takeda, S.: "Cultured green cells of tobacco as a useful material for the study of chloroplast replication"Methods in Cell Sciences. 21. 149-154 (1999)
[Publications] Endo, T.: "The role of chlorplastic NAD(P)H dehy drogenase in photoprotection"FEBS Lett.. 457. 5-8 (1999)
[Publications] Ifuku, K.: "Isolation of cucumber cDNA (accession No. AB03225) for the 23kDa polypeptide of the oxygen evolving complex of photosystem II (psbP) (PGR00-031)"Plant Physiol.. 122. 619 (2000)
[Publications] Murakami, S.: "Protease activity of CND41, a chloroplast nucleoid DNA binding protein isolated from cultured tobacco cells"FEBS Lett.. 468. 15-18 (2000)
[Publications] Sato, F.: "CND41, a DNA binding protein in chloroplast nucleoid, and its function"In "The 13th Symposium on plant Biotechnology ; 1999 Korea Japan Joint Symposium". 13. 51-56 (1999)
[Publications] 佐藤文彦: "葉緑体機能と個体統御"蛋白質核酸酵素. 45. 147-152 (2000)
[Publications] 佐藤文彦: "葉緑体構築のダイナミックス序説"蛋白質核酸酵素. 45. 105-107 (2000)
[Publications] Sugita, C.: "Targeted deletion of genes for eukaryotic RNA binding proteins, Rbp1 and Rbp2, in cyanobacterium Synechococcus sp. strain PCC7942 : Rbp1 gene is indispensable for cell growth at low temperatures"FEMS Microbiol. Lett.. 176. 142-147 (1999)
[Publications] Nakamura, T.: "Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs"FEBS Lett.. 460. 437-441 (1999)
[Publications] Mutsuda, M.: "Physiological characterization of RNA-binding protein ?deficient cells from Synechococcus sp. strain PCC7942"Plant Cell Physiol.. 40. 1203-1209 (1999)
[Publications] Sugita, C.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp. PCC6301 : a 33 kDa soluble polypeptide with high sequence similarity to ribosomal protein S1."Mol. Gen. Genet.. (in press).
[Publications] 杉田護: "遺伝子遺伝子発現における転写後調節"蛋白質核酸酵素. 45. 132-138 (2000)
[Publications] Omata, T.: "Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942"Proc. Natl. Acad. Sci. USA. 96(23). 13571-13576 (1999)
[Publications] Akashi, T.: "Comparison of the electrostatic binding sit es on the surface of ferredoxin for two ferredoxin-dependent enzymes, ferredoxin-NADP+ reductase and sulfite reductase"J. Biol. Chem.. 274. 29399-29405 (1999)
[Publications] Matsumura, T.: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves"Plant Physiol.. 119. 481-488 (1999)
[Publications] Okuhara, H.: "Cloning and inactive ation of genes encoding ferredoxin and NADH-dependent glutamate synthases in the cyanobacterium Plectonema boryanum. Imbalances in nitrogen and carbon assimilations caused by deficiency of the ferredoxin dependent enzymes"Plant Physiol.. 120. 33-41 (1999)
[Publications] Yonekura-Sakakibara, K.: "Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize"Plant Physiol.. 122. 887-894 (2000)
[Publications] Yamada, K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme"J. Biol. Chem.. 274. 2563-2570 (1999)
[Publications] Hayashi, M.: "Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase"Plant Cell Physiol.. 40. 263-272 (1999)
[Publications] Mano, S.: "Light regulates alternative splicing of hydoroxypyru vate reductase in pumpkin"Plant J.. 17. 309-320 (1999)
[Publications] Koumoto, Y.: "Chloroplast Cpn 20 forms a tetrameric structure in Arabidopsis thaliana"Plant J.. 17. 467-477 (1999)
[Publications] Hiraiwa, N.: "Vacuolar processing enzyme is activated self-catalytically by sequential removal of the C-terminal and N-terminal propeptides"FEBS Lett.. 447. 213-216 (1999)
[Publications] Hayashi, H.: "A novel oxidase that catalyzes oxidation of short-chain acyl CoA in plant peroxisomes"J. Biol. Chem.. 274. 12715-12721 (1999)
[Publications] Kato, A.: "Oligomeric proteins containing N-terminal targeting signals are imported into peroxisomes in transgenic Arabidopsis"Plant Cell Physiol.. 40. 586-591 (1999)
[Publications] Kinoshita, T.: "Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions"Plant J.. 19. 43-53 (1999)
[Publications] De Bellis L.: "Purification and characterization of pumpkin long-chain acyl-CoA oxidase"Plant Physiol.. 106. 170-176 (1999)
[Publications] Takemoto, D.: "Molecular cloning of a defense-response-related cytochrome P450 gene from tobacco using a fungal elicitor"Plant Cell Physiol.. (in press).
[Publications] De Bellis, L.: "Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase"Plant Physiol.. (in press).

1999 Fiscal Year Annual Research Report

光合成機能統御の細胞内コミュニケーション機構

Principal Investigator

佐藤 文彦 京都大学, 生命科学研究科, 教授 (10127087)

Research Products

[Publications] Takeda, S.: "Cultured green cells of tobacco as a useful material for the study of chloroplast replication"Methods in Cell Sciences. 21. 149-154 (1999)

[Publications] Endo, T.: "The role of chlorplastic NAD(P)H dehy drogenase in photoprotection"FEBS Lett.. 457. 5-8 (1999)

[Publications] Ifuku, K.: "Isolation of cucumber cDNA (accession No. AB03225) for the 23kDa polypeptide of the oxygen evolving complex of photosystem II (psbP) (PGR00-031)"Plant Physiol.. 122. 619 (2000)

[Publications] Murakami, S.: "Protease activity of CND41, a chloroplast nucleoid DNA binding protein isolated from cultured tobacco cells"FEBS Lett.. 468. 15-18 (2000)

[Publications] Sato, F.: "CND41, a DNA binding protein in chloroplast nucleoid, and its function"In "The 13th Symposium on plant Biotechnology ; 1999 Korea Japan Joint Symposium". 13. 51-56 (1999)

[Publications] 佐藤文彦: "葉緑体機能と個体統御"蛋白質核酸酵素. 45. 147-152 (2000)

[Publications] 佐藤文彦: "葉緑体構築のダイナミックス 序説"蛋白質核酸酵素. 45. 105-107 (2000)

[Publications] Sugita, C.: "Targeted deletion of genes for eukaryotic RNA binding proteins, Rbp1 and Rbp2, in cyanobacterium Synechococcus sp. strain PCC7942 : Rbp1 gene is indispensable for cell growth at low temperatures"FEMS Microbiol. Lett.. 176. 142-147 (1999)

[Publications] Nakamura, T.: "Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs"FEBS Lett.. 460. 437-441 (1999)

[Publications] Mutsuda, M.: "Physiological characterization of RNA-binding protein ?deficient cells from Synechococcus sp. strain PCC7942"Plant Cell Physiol.. 40. 1203-1209 (1999)

[Publications] Sugita, C.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp. PCC6301 : a 33 kDa soluble polypeptide with high sequence similarity to ribosomal protein S1."Mol. Gen. Genet.. (in press).

[Publications] 杉田護: "遺伝子遺伝子発現における転写後調節"蛋白質 核酸 酵素. 45. 132-138 (2000)

[Publications] Omata, T.: "Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942"Proc. Natl. Acad. Sci. USA. 96(23). 13571-13576 (1999)

[Publications] Akashi, T.: "Comparison of the electrostatic binding sit es on the surface of ferredoxin for two ferredoxin-dependent enzymes, ferredoxin-NADP+ reductase and sulfite reductase"J. Biol. Chem.. 274. 29399-29405 (1999)

[Publications] Matsumura, T.: "Complementary DNA cloning and characterization of ferredoxin localized in bundle-sheath cells of maize leaves"Plant Physiol.. 119. 481-488 (1999)

[Publications] Yonekura-Sakakibara, K.: "Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize"Plant Physiol.. 122. 887-894 (2000)

[Publications] Yamada, K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme"J. Biol. Chem.. 274. 2563-2570 (1999)

[Publications] Hayashi, M.: "Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase"Plant Cell Physiol.. 40. 263-272 (1999)

[Publications] Mano, S.: "Light regulates alternative splicing of hydoroxypyru vate reductase in pumpkin"Plant J.. 17. 309-320 (1999)

[Publications] Koumoto, Y.: "Chloroplast Cpn 20 forms a tetrameric structure in Arabidopsis thaliana"Plant J.. 17. 467-477 (1999)

[Publications] Hiraiwa, N.: "Vacuolar processing enzyme is activated self-catalytically by sequential removal of the C-terminal and N-terminal propeptides"FEBS Lett.. 447. 213-216 (1999)

[Publications] Hayashi, H.: "A novel oxidase that catalyzes oxidation of short-chain acyl CoA in plant peroxisomes"J. Biol. Chem.. 274. 12715-12721 (1999)

[Publications] Kato, A.: "Oligomeric proteins containing N-terminal targeting signals are imported into peroxisomes in transgenic Arabidopsis"Plant Cell Physiol.. 40. 586-591 (1999)

[Publications] Kinoshita, T.: "Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions"Plant J.. 19. 43-53 (1999)

[Publications] De Bellis L.: "Purification and characterization of pumpkin long-chain acyl-CoA oxidase"Plant Physiol.. 106. 170-176 (1999)

[Publications] Takemoto, D.: "Molecular cloning of a defense-response-related cytochrome P450 gene from tobacco using a fungal elicitor"Plant Cell Physiol.. (in press).

[Publications] De Bellis, L.: "Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase"Plant Physiol.. (in press).

佐藤文彦京都大学, 生命科学研究科, 教授 (10127087)

[Publications] 佐藤文彦: "葉緑体構築のダイナミックス序説"蛋白質核酸酵素. 45. 105-107 (2000)

[Publications] 杉田護: "遺伝子遺伝子発現における転写後調節"蛋白質核酸酵素. 45. 132-138 (2000)