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2001 Fiscal Year Final Research Report Summary

Detection and manipulation of single bio-motor molecules : Technical innovation and applications.

Research Project

Project/Area Number 09279104
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo (2000-2001)
Keio University (1997-1999)

Principal Investigator

KAMIMURA Shinji  Graduate School of Arts and Sciences, the University of Tokyo Associate Professor, 大学院・総合文化研究科, 助教授 (90177585)

Co-Investigator(Kenkyū-buntansha) SHIMAMOTO Nobuo  National Institute of Genetics Professor, 教授 (20127658)
YANAGIDA Toshio  Graduate School of Medicine, Osaka University Professor, 医学系研究科, 教授 (30089883)
KINOSHITA Kazuhiko  Institute for Molecular Science, Okazaki National Research Institute Professor, 教授 (30124366)
OIWA Kazuhiro  Kansai Advanced Research Center, Communications Research Laboratory Researcher, 通信総合研究所, 室長(研究職)
上田 太朗  工業技術院, 産業技術融合領域研究所, 主任研究員
UYEDA Taro q.p.  National Institute for Advanced Interdisciplinary Research Researcher
Project Period (FY) 1997 – 2000
Keywordsmyosin / dynein / single molecule detection and analysis / biomotor / mechanism of cell motility / chemo-mechanical coupling / F1-ATPase / trp repressor
Research Abstract

During grant support for the project we executed several different kinds of research works on the biophysical and biochemical features of bio-motors. In particular our efforts were paid to develop novel techniques that enable us to approach directly to single molecular events. K.K. developed a novel technique to detect molecular orientation from fluorescence polarization, which was applied to observe helical motions of actin filaments on myosin fibers. T.Y. improved his technique to detect a single ATP molecule during force development by myosin and the applied experiments showed ca.100 ms of delay for the force generation after ATP binding suggesting an existence of energy-storing state of myosin. He also improved micro glass-needle technique to analyze force-velocity relationship of a single myosin under various experimental conditions. S.K. developed a new technique to record FRET of single molecules with real time resolution (ca.10 Hz time resolution). New trials using various kind … More s of other bio-motors were also carried out during the project. N.S. showed that sliding motion of E. coli trp-repressor on DNA strands enhanced its affinity towards specific DNA sequences and has started single-molecule analysis. Inter-molecular collision among RNA polymerases was also shown by N.S. to be very crucial for the initial formation of short RNA fragments. K.K found that the rotation steps of F1-ATPases being labeled with fluorescent actin was just 120 degrees and the observed speed was as high as 100 Hz. Although the following approaches were not such experiments using single bio-motors but new studies on various types of motor proteins were carried out. S.K. applied a caged-ATP technique to analyze force develapment by axonemal dynein and showed that dynein-ADP was the force-generating intermediate of during ATP hydrolysis. K.O. analyzed detailed features of inner dynein of Chlamydomonas. He found that dynein subspecies f was a motor molecule with an exceptionally high duty-ratio and that subspecies c was a processive motor. T.Q.P.U. found co-operativity between two head domains of myosin from the analysis of single-headed myosin motors. He also found cross bridge could be highly stabilized by G680V mutation in Dictyostelium myosin. Other novel techniques were developed during the project, e.g. novel fluorescent ATP analogues to detect the conformation changes of myosin (K.O.), an artificial system to reconstruct the active directional flow by bio-motors (T.Q.P.U.), a new AFM technique using sensitive-probes to detect surface charge differences (T.Y.), AFM detection of bio-oscillatory motion (S.K.) should be still preliminary but further studies for the applications should be awaited. Less

  • Research Products

    (156 results)

All Other

All Publications (156 results)

  • [Publications] Harada, Y, Ohara, O, Takatsuki, A, Itoh, H, Shimamoto, N, Kinosita, K: "Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase"NATURE. 409. 113-115 (2001)

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  • [Publications] Hiratsuka, Y, Tada, T, Oiwa, K, Kanayama, T, Uyeda, TQP: "Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks"BIOPHYSICAL JOURNAL. 81. 1555-1561 (2001)

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  • [Publications] Inoue, Y, Iwane, AH, Miyai, T, Muto, E, Yanagida, T: "Motility of single one-headed kinesin molecules along microtubules"BIOPHYSICAL JOURNAL. 81. 2838-2850 (2001)

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  • [Publications] Ishii, Y, Ishijima, A, Yanagida, T: "Single molecule nanomanipulation of biomolecules"TRENDS IN BIOTECHNOLOGY. 19. 211-216 (2001)

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  • [Publications] Ishijima, A, Yanagida, T: "Single molecule nanobioscience"TRENDS IN BIOCHEMICAL SCIENCES. 26. 438-444 (2001)

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  • [Publications] Iwamoto, H, Oiwa, K, Suzuki, T, Fujisawa, T: "X-ray diffraction evidence for the lack of stereospecific protein interactions in highly activated actomyosin complex"JOURNAL OF MOLECULAR BIOLOGY. 305. 863-874 (2001)

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  • [Publications] Nishiyama, M, Muto, E, Inoue, Y, Yanagida, T, Higuchi, H: "Substeps within the 8-nm step of the ATPase cycle of single kinesin molecules"NATURE CELL BIOLOGY. 3. 425-428 (2001)

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  • [Publications] Takahashi, M, Takahashi, K, Hiratsuka, Y, Uchida, K, Yamagishi, A, Uyeda, TQP, Yazawa, M: "Functional characterization of vertebrate nonmuscle myosin IIB isoforms using Dictyostelium chimeric myosin II"JOURNAL OF BIOLOGICAL CHEMISTRY. 276. 1034-1040 (2001)

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  • [Publications] Ueda, M, Sako, Y, Tanaka, T, Devreotes, P, Yanagida, T: "Single-molecule analysis of chemotactic signaling in Dictyostelium cells"SCIENCE. 294. 864-867 (2001)

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  • [Publications] Yamada, A, Yoshio, M, Kojima, H, Oiwa, K: "An in vitro assay reveals essential protein components for the"catch"state of invertebrate smooth muscle"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 98. 6635-6640 (2001)

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  • [Publications] Yasuda, R, Noji, H, Yoshida, M, Kinoshita, K, Itoh, H: "Resolution of distinct rotational substeps by submillisecond kinetic analysis of F-I-ATPase"NATURE. 410. 898-904 (2001)

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  • [Publications] Fukui, Y, Uyeda, TQP, Kitayama, C, Inoue, S: "How well can an amoeba climb?"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 97. 10020-10025 (2000)

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  • [Publications] Ishii, Y, Kimura, Y, Kitamura, K, Tanaka, H, Wazawa, T, Yanagida, T: "Imaging and nano-manipulation of single actomyosin motors at work"CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY. 27. 229-237 (2000)

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  • [Publications] Kaseda, K, Yokota, H, Ishii, Y, Yanagida, T, Inoue, T, Fukui, K, Kodama, T: "Single-molecule imaging of interaction between dextran and glucosyltransferase from Streptococcus sobrinus"JOURNAL OF BACTERIOLOGY. 182. 1162-1166 (2000)

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  • [Publications] Koyama, S, Kamimura, S: "Influence of social dominance and female odor on the sperm activity of male mice"PHYSIOLOGY & BEHAVIOR. 71. 415-422 (2000)

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  • [Publications] Liu, X, Ito, K, Lee, RJ, Uyeda, TQP: "Involvement of tail domains in regulation of Dictyostelium myosin II"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 271. 75-81 (2000)

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  • [Publications] Matsumoto, T, Morimoto, Y, Shibata, N, Kinebuchi, T, Shimamoto, N, Tsukihara, T, Yasuoka, N: "Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by x-ray structure analysis"JOURNAL OF BIOCHEMISTRY. 127. 329-335 (2000)

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  • [Publications] Miyamoto, Y, Muto, E, Mashimo, T, Iwane, AH, Yoshiya, I, Yanagida, T: "Direct inhibition of microtubule-based kinesin motility by local anesthetics"BIOPHYSICAL JOURNAL. 78. 940-949 (2000)

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  • [Publications] Muramatsu, H, Homma, K, Yamamoto, N, Wang, J, Sakata-Sogawa, K, Shimamoto, N: "Imaging of DNA molecules by scanning near-field microscope"MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS. 12. 29-32 (2000)

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  • [Publications] Oiwa, K, Eccleston, JF, Anson, M, Kikumoto, M, Davis, CT, Reid, GP, Ferenczi, MA, Corrie, JET, Yamada, A, Nakayama H, Trentham, DR: "Comparative single-molecule and ensemble myosin enzymology:Sulfoindocyanine ATP and ADP derivatives"BIOPHYSICAL JOURNAL. 78. 3048-3071 (2000)

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  • [Publications] Sako, Y, Minoghchi, S, Yanagida, T: "Single-molecule imaging of EGFR signalling on the surface of living cells"NATURE CELL BIOLOGY. 2. 168-172 (2000)

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  • [Publications] Sen, R, Nagai, H, Shimamoto, N: "Polymerase arrest at the lambda P-R promoter during transcription initiation"JOURNAL OF BIOLOGICAL CHEMISTRY. 275. 10899-10904 (2000)

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  • [Publications] Shimizu, S, Ide, T, Yanagida, T, Tsujimoto, Y: "Electrophysiological study of a novel large pore formed by Bax and the voltage-dependent anion channel that is permeable to cytochromec"JOURNAL OF BIOLOGICAL CHEMISTRY. 275. 12321-12325 (2000)

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  • [Publications] Uyeda, TQP, Kitayama, C, Yumura, S: "Myosin II-independent cytokinesis in Dictyostelium:its mechanism and implications"CELL STRUCTURE AND FUNCTION. 25. 1-10 (2000)

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  • [Publications] Uyeda, TQP, Yumura, S: "Molecular biological approaches to study myosin functions in cytokinesis of Dictyostelium"MICROSCOPY RESEARCH AND TECHNIQUE. 49. 136-144 (2000)

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  • [Publications] Wazawa, T, Ishii, Y, Funatsu, T, Yanagida, T: "Spectral fluctuation of a single fluorophore conjugated to a protein molecule"BIOPHYSICAL JOURNAL. 78. 1561-1569 (2000)

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  • [Publications] Yamada, A, Yoshio, M, Oiwa, K, Nyitray, L: "Catchin,a novel protein in molluscan catch muscles,is produced by alternative splicing from the myosin heavy chain gene"JOURNAL OF MOLECULAR BIOLOGY. 295. 169-178 (2000)

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  • [Publications] Yamamoto, T, Kurosawa, O, Kabata, H, Shimamoto, N, Washizu, M: "Molecular surgery of DNA based on electrostatic micromanipulation"IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS. 36. 1010-1017 (2000)

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  • [Publications] Yanagida, T, Esaki, S, Iwane, AH, Inoue, Y, Ishijima, A, Kitamura, K, Tanaka, H, Tokunaga, M: "Single-motor mechanics and models of the myosin motor"PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES. 355. 441-447 (2000)

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  • [Publications] Yanagida, T, Iwane, AH: "A large step for myosin"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 97. 9357-9359 (2000)

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  • [Publications] Yanagida, T, Kitamura, K, Tanaka, H, Iwane, AH, Esaki, S: "Single molecule analysis of the actomyosin motor"CURRENT OPINION IN CELL BIOLOGY. 12. 20-25 (2000)

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  • [Publications] Fukui, Y, Uyeda, TQP, Kitayama, C, Inoue, S: "Migration forces in Dictyostelium measured by centrifuge DIC microscopy"BIOLOGICAL BULLETIN. 197. 260-262 (1999)

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  • [Publications] Harada, Y, Funatsu, T, Murakami, K, Nonoyama, Y, Ishihama, A, Yanagida, T: "Single-molecule imaging of RNA polymerase-DNA interactions in real time"BIOPHYSICAL JOURNAL. 76. 709-715 (1999)

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  • [Publications] Ide, T, Yanagida, T: "An artificial lipid bilayer formed on an agarose-coated glass for simultaneous electrical and optical measurement of single ion channels"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 265. 595-599 (1999)

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  • [Publications] Ishii, Y, Yoshida, T, Funatsu, T, Wazawa, T, Yanagida, T: "Fluorescence resonance energy transfer between single fluorophores attached to a coiled-coil protein in aqueous solution"CHEMICAL PHYSICS. 247. 163-173 (1999)

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  • [Publications] Ishijima, A, Kojima, H, Tanaka, H, Yanagida, T: "Simultaneous measurement of individual ATPase and mechanical reactions by a single myosin molecule at work"OPTICAL REVIEW. 6. 16-23 (1999)

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  • [Publications] Ito, K, Liu, X, Katayama, E, Uyeda, TQP: "Cooperativity between two heads of Dictyostelium myosin II in in vitro motility and ATP hydrolysis"BIOPHYSICAL JOURNAL. 76. 985-992 (1999)

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  • [Publications] Iwatani, S, Iwane, AH, Higuchi, H, Ishii, Y, Yanagida, T: "Mechanical and chemical properties of cysteine-modified kinesin molecules"BIOCHEMISTRY. 38. 10318-10323 (1999)

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  • [Publications] Kinoshita, K, Atarashi, H, Ishikawa, Y, Onoe, S, Nakamura, Y, Nakagawa, M: "Analysis of the relation between chip rate and capacity in DS/CDMA cellular systems considering adjacent channel interference"IEICE TRANSACTIONS ON FUNDAMENTALS OF ELECTRONICS COMMUNICATIONS AND COMPUTER SCIENCES. E82A. 2697-2705 (1999)

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  • [Publications] Kitamura, K, Tokunaga, M, Iwane, AH, Yanagida, T: "A single myosin head moves along an actin filament with regular steps of 5.3 nanometres"NATURE. 397. 129-134 (1999)

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  • [Publications] Knetsch, MLW, Uyeda, TQP, Manstein, DJ: "Disturbed communication between actin-and nucleotide-binding sites in a myosin II with truncated 50/20-kDa junction"JOURNAL OF BIOLOGICAL CHEMISTRY. 274. 20133-20138 (1999)

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  • [Publications] Koyama, S, Kamimura, S: "Lowered sperm motility in subordinate social status of mice"PHYSIOLOGY & BEHAVIOR. 65. 665-669 (1999)

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  • [Publications] Sakakibara, H, Kojima, H, Sakai, Y, Katayama, E, Oiwa, K: "Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor"NATURE. 400. 586-590 (1999)

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  • [Publications] Shimamoto, N: "One-dimensional diffusion of proteins along DNA-Its biological and chemical significance revealed by single- molecule measurements"JOURNAL OF BIOLOGICAL CHEMISTRY. 274. 15293-15296 (1999)

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  • [Publications] Shu, S, Lee, RJ, LeBlanc-Straceski, JM, Uyeda, TQP: "Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium"JOURNAL OF CELL SCIENCE. 112. 2195-2201 (1999)

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  • [Publications] Shu, S, Uyeda, TQP, Liu, AX, Liu, GQ, Yen, LF: "Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum"CHINESE SCIENCE BULLETIN. 44. 811-816 (1999)

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  • [Publications] Tani, T, Kamimura, S: "Dynein-ADP as a force-generating intermediate revealed by a rapid reactivation of flagellar axoneme"BIOPHYSICAL JOURNAL. 77. 1518-1527 (1999)

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  • [Publications] Yamasaki, R, Hoshino, M, Wazawa, T, Ishii, Y, Yanagida, T, Kawata, Y, Higurashi, T, Sakai, K, Nagai, J, Goto, Y: "Single molecular observation of the interaction of GroEL with substrate proteins"JOURNAL OF MOLECULAR BIOLOGY. 292. 965-972 (1999)

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  • [Publications] Ye, LH, Kishi, H, Nakamura, A, Okagaki, T, Tanaka, T, Oiwa, K, Kohama, K: "Myosin light-chain kinase of smooth muscle stimulates myosin ATPase activity without phosphorylating myosin light chain"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 96. 6666-6671 (1999)

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  • [Publications] Harada, Y, Funatsu, T, Tokunaga, M, Saito, K, Higuchi, H, Ishii, Y, Yanagida, T: "Single molecule imaging and nanomanipulation of biomolecules"METHODS IN CELL BIOLOGY,VOL 55. 55. 117-128 (1998)

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  • [Publications] Ishijima, A, Kojima, H, Funatsu, T, Tokunaga, M, Higuchi, H, Tanaka, H, Yanagida, T: "Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin"CELL. 92. 161-171 (1998)

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  • [Publications] Liu, X, Ito, K, Morimoto, S, Hikkoshi-Iwane, A, Yanagida, T, Uyeda, TQP: "Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 95. 14124-14129 (1998)

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  • [Publications] Oiwa, K, Yamaga, T, Yamada, A: "Direct observation of a central bare zone in a native thick filament isolated from the anterior byssus retractor muscle of Mytilus edulis using fluorescent ATP analogue"JOURNAL OF BIOCHEMISTRY. 123. 614-618 (1998)

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  • [Publications] Sen, R, Nagai, H, Hernandez, VJ, Shimamoto, N: "Reduction in abortive transcription from the lambda P-R promoter by mutations in region 3 of the sigma(70)subunit of Escherichia coli RNA polymerase"JOURNAL OF BIOLOGICAL CHEMISTRY. 273. 9872-9877 (1998)

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  • [Publications] Tanaka, H, Ishijima, A, Honda, M, Saito, K, Yanagida, T: "Orientation dependence of displacements by a single one-headed myosin relative to the actin filament"BIOPHYSICAL JOURNAL. 75. 1886-1894 (1998)

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  • [Publications] Tani, T, Kamimura, S: "Reactivation of sea-urchin sperm flagella induced by rapid photolysis of caged ATP"JOURNAL OF EXPERIMENTAL BIOLOGY. 201. 1493-1503 (1998)

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  • [Publications] Watanabe, T, Imoto, M, Taniguchi, H, Kinoshita, K, Umezawa, K: "Synthesis of fluorescent substrates for protein tyrosine phosphatase assays"BIOORGANIC & MEDICINAL CHEMISTRY LETTERS. 8. 1301-1302 (1998)

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  • [Publications] Yamada, A, Yamaga, T, Sakakibara, H, Nakayama, H, Oiwa, K: "Unidirectional movement of fluorescent microtubules on rows of dynein arms of disintegrated axonemes"JOURNAL OF CELL SCIENCE. 111. 93-98 (1998)

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  • [Publications] Yokota, H, Saito, K, Yanagida, T: "Single molecule imaging of fluorescently labeled proteins on metal by surface plasmons in aqueous solution"PHYSICAL REVIEW LETTERS. 80. 4606-4609 (1998)

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  • [Publications] Aoki, T, Hiroshima, M, Kitamura, K, Tokunaga, M, Yanagida, T: "Non-contact scanning probe microscopy with sub-piconewton force sensitivity"ULTRAMICROSCOPY. 70. 45-55 (1997)

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  • [Publications] Funatsu, T, Harada, Y, Higuchi, H, Tokunaga, M, Saito, K, Ishii, Y, Vale, RD, Yanagida, T: "Imaging and nano-manipulation of single biomolecules"BIOPHYSICAL CHEMISTRY. 68. 63-72 (1997)

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  • [Publications] Hashimoto, K, Azuma, C, Tokugawa, Y, Nobunaga, T, Aki, TA, Matsui, Y, Yanagida, T, Izumi, H, Saji, F, Murata, Y: "Loss of H19 imprinting and up-regulation of H19 and SNRPN in a case with malignant mixed Mullerian tumor of the uterus"HUMAN PATHOLOGY. 28. 862-865 (1997)

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  • [Publications] Hatada, K, Kitayama, T, Ute, K, Terawaki, Y, Yanagida, T: "End-group analysis of poly(methyl methacrylate)prepared with benzoyl peroxide by 750 MHz high-resolution H-1 NMR spectroscopy"MACROMOLECULES. 30. 6754-6759 (1997)

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  • [Publications] Higuchi, H, Muto, E, Inoue, Y, Yanagida, T: "Kinetics of force generation by single kinesin molecules activated by laser photolysis of caged ATP"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 94. 4395-4400 (1997)

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  • [Publications] Inoue, Y, Toyoshima, YY, Iwane, AH, Morimoto, S, Higuchi, H, Yanagida, T: "Movements of truncated kinesin fragments with a short or an artificial flexible neck"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 94. 7275-7280 (1997)

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  • [Publications] Iwane, AH, Funatsu, T, Harada, Y, Tokunaga, M, Ohara, O, Morimoto, S, Yanagida, T: "Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in vitro"FEBS LETTERS. 407. 235-238 (1997)

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      「研究成果報告書概要(和文)」より
  • [Publications] Iwane, AH, Kitamura, K, Tokunaga, M, Yanagida, T: "Myosin subfragment-1 is fully equipped with factors essential for motor function"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 230. 76-80 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinebuchi, T, Shindo, H, Nagai, H, Shimamoto, N, Shimizu, M: "Functional domains of Escherichia coli single-stranded DNA binding protein as assessed by analyses of the deletion mutants"BIOCHEMISTRY. 36. 6732-6738 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kojima, H, Muto, E, Higuchi, H, Yanagida, T: "Mechanics of single kinesin molecules measured by optical trapping nanometry"BIOPHYSICAL JOURNAL. 73. 2012-2022 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubori, T, Shimamoto, N: "Physical interference between Escherichia coli RNA polymerase molecules transcribing in tandem enhances abortive synthesis and misincorporation"NUCLEIC ACIDS RESEARCH. 25. 2640-2647 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai, H, Shimamoto, N: "Regions of the Escherichia coli primary sigma factor alpha(70)that are involved in interaction with RNA polymerase core enzyme"GENES TO CELLS. 2. 725-734 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito, K, Tokunaga, M, Iwane, AH, Yanagida, T: "Dual-colour microscopy of single fluorophores bound to myosin interacting with fluorescently labelled actin using anti-Stokes fluorescence"JOURNAL OF MICROSCOPY-OXFORD. 188. 255-263 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki, H, Yamada, A, Oiwa, K, Nakayama, H, Mashiko, S: "Control of actin moving trajectory by patterned poly(methyl methacrylate)tracks"BIOPHYSICAL JOURNAL. 72. 1997-2001 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki, M, Shigematsu, J, Fukunishi, Y, Harada, Y, Yanagida, T, Kodama, T: "Coupling of protein surface hydrophobicity change to ATP hydrolysis by myosin motor domain"BIOPHYSICAL JOURNAL. 72. 18-23 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki, Y, Ohkura, R, Sugiura, S, Yasuda, R, Kinoshita, K, Tanokura, M, Sutoh, K: "Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 234. 701-706 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga, M, Kitamura, K, Saito, K, Iwane, AH, Yanagida, T: "Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 235. 47-53 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yumura, S, Uyeda, TQP: "Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells"MOLECULAR BIOLOGY OF THE CELL. 8. 2089-2099 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yumura, S, Uyeda, TQP: "Myosin II can be localized to the cleavage furrow and to the posterior region of Dictyostelium amoebae without control by phosphorylation of myosin heavy and light chains"CELL MOTILITY AND THE CYTOSKELETON. 36. 313-322 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada, Y Ohara, O Takatsuki, A Itoh, H Shimamoto, N Kinosita, K: "Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase"NATURE. 409. 113-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiratsuka, Y Tada, T Oiwa, K Kanayama, T Uyeda, TQP: "Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks"BIOPHYSICAL JOURNAL. 81. 1555-1561 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, Y Iwane, AH Miyai, T Muto, E Yanagida, T: "Motility of single one-headed kinesin molecules along microtubules"BIOPHYSICAL JOURNAL. 81. 2838-2850 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii, Y Ishijima, A Yanagida, T: "Single molecule nanomanipulation of biomolecules"TRENDS IN BIOTECHNOLOGY. 19. 211-216 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishijima, A Yanagida, T: "Single molecule nanobioscience"TRENDS IN BIOCHEMICAL SCIENCES. 26. 438-444 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwamoto, H Oiwa, K Suzuki, T Fujisawa, T: "X-ray diffraction evidence for the lack of stereospecific protein interactions in highly activated actomyosin complex"JOURNAL OF MOLECULAR BIOLOGY. 305. 863-874 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishiyama, M Muto, E Inoue, Y Yanagida, T Higuchi, H: "Substeps within the 8-nm step of the ATPase cycle of single kinesin molecules"NATURE CELL BIOLOGY. 3. 425-428 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, M Takahashi, K Hiratsuka, Y Uchida, K Yamagishi, A Uyeda, TQP Yazawa, M: "Functional characterization of vertebrate nonmuscle myosin IIB isoforms using Dictyostelium chimeric myosin II"JOURNAL OF BIOLOGICAL CHEMISTRY. 276. 1034-1040 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, M Sako, Y Tanaka, T Devreotes, P Yanagida, T: "Single-molecule analysis of chemotactic signaling in Dictyostelium cells"SCIENCE. 294. 864-867 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, A Yoshio, M Kojima, H Oiwa, K: "An in vitro assay reveals essential protein components for the "catch" state of invertebrate smooth muscle"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 98. 6635-6640 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuda, R Noji, H Yoshida, M Kinoshita, K Itoh, H: "Resolution of distinct rotational substeps by submillisecond kinetic analysis of F-1-ATPase"NATURE. 410. 898-904 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukui, Y Uyeda, TQP Kitayama, C Inoue, S: "How well can an amoeba climb?"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 97. 10020-10025 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii, Y Kimura, Y Kitamura, K Tanaka, H Wazawa, T Yanagida, T: "Imaging and nano-manipulation of single actomyosin motors at work"CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY. 27. 229-237 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaseda, K Yokota, H Ishii, Y Yanagida, T Inoue, T Fukui, K Kodama, T: "Single-molecule imaging of interaction between dextran and glucosyltransferase from Streptococcus sobrinus"JOURNAL OF BACTERIOLOGY. 182. 1162-1166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyama, S Kamimura, S: "Influence of social dominance and female odor on the sperm activity of male mice"PHYSIOLOGY & BEHAVIOR. 71. 415-422 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Liu, X Ito, K Lee, RJ Uyeda, TQP: "Involvement of tail domains in regulation of Dictyostelium myosin II"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 271. 75-81 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, T Morimoto, Y Shibata, N Kinebuchi, T Shimamoto, N Tsukihara, T Yasuoka, N: "Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by x-ray structure analysis"JOURNAL OF BIOCHEMISTRY. 127. 329-335 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, Y Muto, E Mashimo, T Iwane, AH Yoshiya, I Yanagida, T: "Direct inhibition of microtubule-based kinesin motility by local anesthetics"BIOPHYSICAL JOURNAL. 78. 940-949 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muramatsu, H Homma, K Yamamoto, N Wang, J Sakata-Sogawa, K Shimamoto, N: "Imaging of DNA molecules by scanning near-field microscope"MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS. 12. 29-32 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oiwa, K Eccleston, JF Anson, M Kikumoto, M Davis, CT Reid, GP Ferenczi, MA Corrie, JET Yamada, A Nakayama, H Trentham, DR: "Comparative single-molecule and ensemble myosin enzymology : Sulfoindocyanine ATP and ADP derivatives"BIOPHYSICAL JOURNAL. 78. 3048-3071 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sako, Y Minoghchi, S Yanagida, T: "Single-molecule imaging of EGFR signalling on the surface of living cells"NATURE CELL BIOLOGY. 2. 168-172 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sen, R Nagai, H Shimamoto, N: "Polymerase arrest at the lambda P-R promoter during transcription initiation"JOURNAL OF BIOLOGICAL CHEMISTRY. 275. 10899-10904 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, S Ide, T Yanagida, T Tsujimoto, Y: "Electrophysiological study of a novel large pore formed by Bax and the voltage-dependent anion channel that is permeable to cytochrome c"JOURNAL OF BIOLOGICAL CHEMISTRY. 275. 12321-12325 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyeda, TQP Kitayama, C Yumura, S: "Myosin II-independent cytokinesis in Dictyostelium : its mechanism and implications"CELL STRUCTURE AND FUNCTION. 25. 1-10 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyeda, TQP Yumura, S: "Molecular biological approaches to study myosin functions in cytokinesis of Dictyostelium"MICROSCOPY RESEARCH AND TECHNIQUE. 49. 136-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wazawa, T Ishii, Y Funatsu, T Yanagida, T: "Spectral fluctuation of a single fluorophore conjugated to a protein molecule"BIOPHYSICAL JOURNAL. 78. 1561-1569 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, A Yoshio, M Oiwa, K Nyitray, L: "Catchin, a novel protein in molluscan catch muscles, is produced by alternative splicing from the myosin heavy chain gene"JOURNAL OF MOLECULAR BIOLOGY. 295. 169-178 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, T Kurosawa, O Kabata, H Shimamoto, N Washizu, M: "Molecuiar surgery of DNA based on electrostatic micromanipulation"IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS. 36. 1010-1017 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanagida, T Esaki, S Iwane, AH Inoue, Y Ishijima, A Kitamura, K Tanaka, H Tokunaga, M: "Single-motor mechanics and models of the myosin motor"PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES. 355. 441-447 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanagida, T Iwane, AH: "A large step for myosin"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 97. 9357-9359 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanagida, T Kitamura, K Tanaka, H Iwane, AH Esaki, S: "Single molecule analysis of the actomyosin motor"CURRENT OPINION IN CELL BIOLOGY. 12. 20-25 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukui, Y Uyeda, TQP Kitayama, C Inoue, S: "Migration forces in Dictyostelium measured by centrifuge DIC microscopy"BIOLOGICAL BULLETIN. 197. 260-262 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, Y Funatsu, T Murakami, K Nonoyama, Y Ishihama, A Yanagida, T: "Single-molecule imaging of RNA polymerase-DNA interactions in real time"BIOPHYSICAL JOURNAL. 76. 709-715 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ide, T Yanagida, T: "An artificial lipid bilayer formed on an agarose-coated glass for simultaneous electrical and optical measurement of single ion channels"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 265. 595-599 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii, Y Yoshida, T Funatsu, T Wazawa, T Yanagida, T: "Fluorescence resonance energy transfer between single fluorophores attached to a coiled-coil protein in aqueous solution"CHEMICAL PHYSICS. 247. 163-173 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishijima, A Kojima, H Tanaka, H Yanagida, T: "Simultaneous measurement of individual ATPase and mechanical reactions by a single myosin molecule at work"OPTICAL REVIEW. 6. 16-23 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, K Liu, X Katayama, E Uyeda, TQP: "Cooperativity between two heads of Dictyostelium myosin II in in vitro motility and ATP hydrolysis"BIOPHYSICAL JOURNAL. 76. 985-992 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwatani, S Iwane, AH Higuchi, H Ishii, Y Yanagida, T: "Mechanical and chemical properties of cysteine-modified kinesin molecules"BIOCHEMISTRY. 38. 10318-10323 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita, K Atarashi, H Ishikawa, Y Onoe, S Nakamura, Y Nakagawa, M: "Analysis of the relation between chip rate and capacity in DS/CDMA cellular systems considering adjacent channel interference"IEICE TRANSACTIONS ON FUNDAMENTALS OF ELECTRONICS COMMUNICATIONS AND COMPUTER SCIENCES. E82A. 2697-2705 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitamura, K Tokunaga, M Iwane, AH Yanagida, T: "A single myosin head moves along an actin filament with regular steps of 5.3 nanometres"NATURE. 397. 129-134 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Knetsch, MLW Uyeda, TQP Manstein, DJ: "Disturbed communication between actin- and nucleotide-binding sites in a myosin II with truncated 50/20-kDa junction"JOURNAL OF BIOLOGICAL CHEMISTRY. 274. 20133-20138 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyama, S Kamimura, S: "Lowered sperm motility in subordinate social status of mice"PHYSIOLOGY & BEHAVIOR. 65. 665-669 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakakibara, H Kojima, H Sakai, Y Katayama, E Oiwa, K: "Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor"NATURE. 400. 586-590 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimamoto, N: "One-dimensional diffusion of proteins along DNA - Its biological and chemical significance revealed by single- molecule measurements"JOURNAL OF BIOLOGICAL CHEMISTRY. 274. 15293-15296 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shu, S Lee, RJ LeBlanc-Straceski, JM Uyeda, TQP: "Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium"JOURNAL OF CELL SCIENCE. 112. 2195-2201 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shu, S Uyeda, TQP Liu, AX Liu, GQ Yen, LF: "Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum"CHINESE SCIENCE BULLETIN. 44. 811-816 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tani, T Kamimura, S: "Dynein-ADP as a force-generating intermediate revealed by a rapid reactivation of flagellar axoneme"BIOPHYSICAL JOURNAL. 77. 1518-1527 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamasaki, R Hoshino, M Wazawa, T Ishii, Y Yanagida, T Kawata, Y Higurashi, T Sakai, K Nagai, J Goto, Y: "Single molecular observation of the interaction of GroEL with substrate proteins"JOURNAL OF MOLECULAR BIOLOGY. 292. 965-972 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ye, LH Kishi, H Nakamura, A Okagaki, T Tanaka, T Oiwa, K Kohama, K: "Myosin light-chain kinase of smooth muscle stimulates myosin ATPase activity without phosphorylating myosin light chain"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 96. 6666-6671 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada, Y Funatsu, T Tokunaga, M Saito, K Higuchi, H Ishii, Y Yanagida, T: "Single molecule imaging and nanomanipulation of biomolecules"METHODS IN CELL BIOLOGY, VOL55. 55. 117-128 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishijima, A Kojima, H Funatsu, T Tokunaga, M Higuchi, H Tanaka, H Yanagida, T: "Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin"CELL. 92. 161-171 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Liu, X Ito, K Morimoto, S Hikkoshi-Iwane, A Yanagida, T Uyeda, TQP: "Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 95. 14124-14129 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oiwa, K Yamaga, T Yamada, A: "Direct observation of a central bare zone in a native thick filament isolated from the anterior byssus retractor muscle of Mytilus edulis using fluorescent ATP analogue"JOURNAL OF BIOCHEMISTRY. 123. 614-618 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sen, R Nagai, H Hernandez, VJ Shimamoto, N: "Reduction in abortive transcription from the lambda P-R promoter by mutations in region 3 of the sigma(70) subunit of Escherichia coli RNA polymerase"JOURNAL OF BIOLOGICAL CHEMISTRY. 273. 9872-9877 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, H Ishijima, A Honda, M Saito, K Yanagida, T: "Orientation dependence of displacements by a single one-headed myosin relative to the actin filament"BIOPHYSICAL JOURNAL. 75. 1886-1894 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tani, T Kamimura, S: "Reactivation of sea-urchin sperm flagella induced by rapid photolysis of caged ATP"JOURNAL OF EXPERIMENTAL BIOLOGY. 201. 1493-1503 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, T Imoto, M Taniguchi, H Kinoshita, K Umezawa, K: "Synthesis of fluorescent substrates for protein tyrosine phosphatase assays"BIOORGANIC & MEDICINAL CHEMISTRY LETTERS. 8. 1301-1302 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, A Yamaga, T Sakakibara, H Nakayama, H Oiwa, K: "Unidirectional movement of fluorescent microtubules on rows of dynein arms of disintegrated axonemes"JOURNAL OF CELL SCIENCE. 111. 93-98 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokota, H Saito, K Yanagida, T: "Single molecule imaging of fluorescently labeled proteins on metal by surface plasmons in aqueous solution"PHYSICAL REVIEW LETTERS. 80. 4606-4609 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aoki, T Hiroshima, M Kitamura, K Tokunaga, M Yanagida, T: "Non-contact scanning probe microscopy with sub-piconewton force sensitivity"ULTRAMICROSCOPY. 70. 45-55 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funatsu, T Harada, Y Higuchi, H Tokunaga, M Saito, K Ishii, Y Vale, RD Yanagida, T: "Imaging and nano-manipulation of single biomolecules"BIOPHYSICAL CHEMISTRY. 68. 63-72 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, K Azuma, C Tokugawa, Y Nobunaga, T Aki, TA Matsui, Y Yanagida, T Izumi, H Saji, F Murata, Y: "Loss of H19 imprinting and up-regulation of H19 and SNRPN in a case with malignant mixed Mullerian tumor of the uterus"HUMAN PATHOLOGY. 28. 862-865 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatada, K Kitayama, T Ute, K Terawaki, Y Yanagida, T: "End-group analysis of poly(methyl methacrylate) prepared with benzoyl peroxide by 750 MHz high-resolution H-1 NMR spectroscopy"MACROMOLECULES. 30. 6754-6759 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Higuchi, H Muto, E Inoue, Y Yanagida, T: "Kinetics of force generation by single kinesin molecules activated by laser photolysis of caged ATP"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 94. 4395-4400 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, Y Toyoshima, YY Iwane, AH Morimoto, S Higuchi, H Yanagida, T: "Movements of truncated kinesin fragments with a short or an artificial flexible neck"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 94. 7275-7280 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwane, AH Funatsu, T Harada, Y Tokunaga, M Ohara, O Morimoto, S Yanagida, T: "Single molecular assay of individual ATP turnover by a myosin- GFP fusion protein expressed in vitro"FEBS LETTERS. 407. 235-238 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwane, AH Kitamura, K Tokunaga, M Yanagida, T: "Myosin subfragment-1 is fully equipped with factors essential for motor function"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 230. 76-80 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinebuchi, T Shindo, H Nagai, H Shimamoto, N Shimizu, M: "Functional domains of Escherichia coli single-stranded DNA binding protein as assessed by analyses of the deletion mutants"BIOCHEMISTRY. 36. 6732-6738 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H Muto, E Higuchi, H Yanagida, T: "Mechanics of single kinesin molecules measured by optical trapping nanometry"BIOPHYSICAL JOURNAL. 73. 2012-2022 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubori, T Shimamoto, N: "Physical interference between Escherichia coli RNA polymerase molecules transcribing in tandem enhances abortive synthesis and misincorporation"NUCLEIC ACIDS RESEARCH. 25. 2640-2647 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai, H Shimamoto, N: "Regions of the Escherichia coli primary sigma factor alpha(70) that are involved in interaction with RNA polymerase core enzyme"GENES TO CELLS. 2. 725-734 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, K Tokunaga, M Iwane, AH Yanagida, T: "Dual-colour microscopy of single fluorophores bound to myosin interacting with fluorescently labelled actin using anti-Stokes fluorescence"JOURNAL OF MICROSCOPY-OXFORD. 188. 255-263 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, H Yamada, A Oiwa, K Nakayama, H Mashiko, S: "Control of actin moving trajectory by patterned poly(methyl methacrylate) tracks"BIOPHYSICAL JOURNAL. 72. 1997-2001 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, M Shigematsu, J Fukunishi, Y Harada, Y Yanagida, T Kodama, T: "Coupling of protein surface hydrophobicity change to ATP hydrolysis by myosin motor domain"BIOPHYSICAL JOURNAL. 72. 18-23 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, Y Ohkura, R Sugiura, S Yasuda, R Kinoshita, K Tanokura, M Sutoh, K: "Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 234. 701-706 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, M Kitamura, K Saito, K Iwane, AH Yanagida, T: "Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 235. 47-53 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yumura, S Uyeda, TQP: "Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells"MOLECULAR BIOLOGY OF THE CELL. 8. 2089-2099 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yumura, S Uyeda, TQP: "Myosin II can be localized to the cleavage furrow and to the posterior region of Dictyostelium amoebae without control by phosphorylation of myosin heavy and light chains"CELL MOTILITY AND THE CYTOSKELETON. 36. 313-322 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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