1998 Fiscal Year Annual Research Report

中性化合物の高感度分離同定用ミセル動電クロマトグラフィー・質量分析法の構築

Research Project

Project/Area Number	09304071
Research Institution	Himeji Institute of Technology
Principal Investigator	寺部茂姫路工業大学, 理学部, 教授 (50115888)
Co-Investigator(Kenkyū-buntansha)	大塚浩二姫路工業大学, 理学部, 助教授 (70183762)
Keywords	キャピラリー電気泳動 / 質量分析法 / CE-MS直接結合法 / ミセル動電クロマトグラフィー / エレクトロスプレーイオン化 / オンライン試料濃縮 / スタッキング / スウィーピング
Research Abstract	本年度も主として3つの研究を平行して進めた。一つはMEKC-MSを行うための準備として,MEKCによる中性試料のオンライン濃縮法についてさらに検討した。昨年度中にスタッキング法により疎水性の強い中性化合物であれば,100倍程度の濃縮が可能となっていた。濃縮機構を検討中に全く新しい原理に基づいた濃縮法であるスウィーピング法を開発できた。疎水性の高い中性化合物では5000倍までのオンライン濃縮が可能となった。ミセルと反対電荷を持つ試料については数十万倍の濃縮も可能であることも明らかになりつつある。これら濃縮法は,化合物選択性があるので,環境試料,生体試料などの特定成分の濃縮に有効になると期待できる。もう一つは本研究で新しく購入した質量分析計であるFinnigan LCQとキャピラリー電気泳動(CE)装置HP 3DCEを用い,質量分析法(CE)-MSシステムの利用して,MEKC-MSの高感度化の検討である。実験を主に担当していた外国人特別研究員が去り,博士課程学生に交代したので,装置の取り扱いに習熟するのに時間がかかり,まだ大きくは進展していない。MS装置にも,CE-MSには不都合な点が明らかになり改良を行った。この研究は次年度に大きく進展できる予定である。上記試料濃縮法と組み合わせて,高感度MEKC-MSシステムを完成する予定である。もう一つは研究協力者の所有する装置を用いたMEKC-MSの研究である。部分注入法,逆移動ミセルを用いる方法,界面活性剤の除去を行わずそのままMEKCを行う等種々の方法を試みたところ,どの方法を用いてもMEKC-MSは可能であった。分離との兼ね合いでどの方法が都合がよいかを決めればよいことが明らかになった。しかし,界面活性剤を除去しない場合,検出感度は他の方法より劣った。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] 尾崎祐人: "エレクトロスプレーイオン化法及び大気圧化学イオン化法によるミセル動電クロマトグラフィー/質量分析法の検討" 分析化学. 46. 421-427 (1997)
[Publications] Pim Muijselaar: "Micelles as Pseudo-Stationary Phases in Micellar Electrokinetic Chromatography" Journal of Chromatography A. 780. 41-61 (1997)
[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography 1.Normal Stacking Mode" Journal of Chromatography A. 781. 119-128 (1997)
[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography II.Reversed Electrode Polarity Stacking Mode." Journal of Chromatography A. 791. 255-267 (1997)
[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography III.Stacking with Reversely Migrating Micelles." Analytical Chemistry. 70. 149-157 (1998)
[Publications] Hiroto ozaki: "On-Line Micellar Electrokinetic Chromatography-Mass Spectrometry with a High-Molecular-Mass Surfactant." Journal of Chromatography A. 794. 317-325 (1998)
[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography IV.Field Enhanced Electrokinetic Injection." Journal of Chromatography A. 798. 251-257 (1998)
[Publications] Pim G.Muijselaar: "Partial-Filling Micellar Electrokinetic Chromatography; On-line Coupling with Mass Spectrometry." Journal of Chromatography A. 802. 3-15 (1998)
[Publications] Yoshihide Tanaka: "Separation of Acidic Enantiomers by Capillary Electrophoresis-Mass Spectrometry Employing a Partial Filling Technique." Journal of Chromatography A. 802. 83-88 (1998)
[Publications] Joselito Quirino: "On-Line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography V.Field Enhanced Sample Injection with Reverse Migrating Micelles." Analytical Chemistry. 70. 1893-1901 (1998)
[Publications] Yoshihide Tanaka: "Analysis of Charged Cyclodextrin Derivatives by On-line Capillary Electrophoresis-Ion Spray-Mass Spectrometry." Analytical Science. 14. 383-388 (1998)
[Publications] Joselito Quirino: "On-line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography VI.Stacking Using Reversed Migrating Micelles and a Water Plug." Journal of Chromatography B. 714. 29-38 (1998)
[Publications] Yoshihide Tanaka: "A Strategy for Selecting Separation Solutions in Capillary Electrophoresis-Mass Spectrometry." Journal of Chromatography A. 817. 49-57 (1998)
[Publications] Koji Otsuka: "Stereoselective Separating and Detection of Phenoxy Acid Herbicide Enantiomers by Cyclodextrin Modified Capillary Zone Electrophohresis-Electrospray Ionization Mass Spectrometry." Journal of Chromatography A. 817. 75-81 (1998)
[Publications] 田中喜秀: "キャピラリー電気泳動/質量分析計による界面活性剤の分析" 分析化学. 47. 563-569 (1998)
[Publications] Joselito P.Quirino: "Exceeding 5000-fold Concentration of Dilute Analytes in Micellar Electrokinetic Chromatography" Science. 282. 465-468 (1998)
[Publications] Joselito P.Quirino: "Sample Stacking of Fast Moving Anions in Capillary Zone Electrophoresis under pH Suppressed Flow." Journal of Chromatography A. (印刷中). (1999)
[Publications] Joselito P.Quirino: "Sweeping of Analyte in Electrokinetic Chromatography." Analytical Chemistry. (印刷中). (1999)
[Publications] Joselito P.Quirino: "The Stacking Boundary in Capillary Electrophoresis." Journal of Microcolumn Separation. (印刷中). (1999)
[Publications] Joselito P. Quirino: "Sample concentration by Sample Stacking and Sweeping Using a Microemulsion and a Single-Isomer Sulfated β-Cyclodextrin as Pseudostationary Phases in Electrokinetic chromatography." Journal of Chromatography A. (印刷中). (1999)
[Publications] Koji Otsuka: "Recent Developments in MEKC-MS." Analusis. 26. M44-M47 (1998)
[Publications] Joselito P.Quirino: "Stacking of Neutral Analytes in Micellar Electrokinetic Chromatography." journal of Capillary Electrophoresis. 233-245 (1997)
[Publications] 尾崎祐人: "進歩総説 CE/MS" ぶんせき. 161-170 (1999)

1998 Fiscal Year Annual Research Report

中性化合物の高感度分離同定用ミセル動電クロマトグラフィー・質量分析法の構築

Principal Investigator

寺部 茂 姫路工業大学, 理学部, 教授 (50115888)

Research Products

[Publications] 尾崎祐人: "エレクトロスプレーイオン化法及び大気圧化学イオン化法によるミセル動電クロマトグラフィー/質量分析法の検討" 分析化学. 46. 421-427 (1997)

[Publications] Pim Muijselaar: "Micelles as Pseudo-Stationary Phases in Micellar Electrokinetic Chromatography" Journal of Chromatography A. 780. 41-61 (1997)

[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography 1.Normal Stacking Mode" Journal of Chromatography A. 781. 119-128 (1997)

[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography II.Reversed Electrode Polarity Stacking Mode." Journal of Chromatography A. 791. 255-267 (1997)

[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography III.Stacking with Reversely Migrating Micelles." Analytical Chemistry. 70. 149-157 (1998)

[Publications] Hiroto ozaki: "On-Line Micellar Electrokinetic Chromatography-Mass Spectrometry with a High-Molecular-Mass Surfactant." Journal of Chromatography A. 794. 317-325 (1998)

[Publications] Joselito P.Quirino: "On-Line Concentration of Neutral Analytes for Micellar electrokinetic Chromatography IV.Field Enhanced Electrokinetic Injection." Journal of Chromatography A. 798. 251-257 (1998)

[Publications] Pim G.Muijselaar: "Partial-Filling Micellar Electrokinetic Chromatography; On-line Coupling with Mass Spectrometry." Journal of Chromatography A. 802. 3-15 (1998)

[Publications] Yoshihide Tanaka: "Separation of Acidic Enantiomers by Capillary Electrophoresis-Mass Spectrometry Employing a Partial Filling Technique." Journal of Chromatography A. 802. 83-88 (1998)

[Publications] Joselito Quirino: "On-Line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography V.Field Enhanced Sample Injection with Reverse Migrating Micelles." Analytical Chemistry. 70. 1893-1901 (1998)

[Publications] Yoshihide Tanaka: "Analysis of Charged Cyclodextrin Derivatives by On-line Capillary Electrophoresis-Ion Spray-Mass Spectrometry." Analytical Science. 14. 383-388 (1998)

[Publications] Joselito Quirino: "On-line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography VI.Stacking Using Reversed Migrating Micelles and a Water Plug." Journal of Chromatography B. 714. 29-38 (1998)

[Publications] Yoshihide Tanaka: "A Strategy for Selecting Separation Solutions in Capillary Electrophoresis-Mass Spectrometry." Journal of Chromatography A. 817. 49-57 (1998)

[Publications] Koji Otsuka: "Stereoselective Separating and Detection of Phenoxy Acid Herbicide Enantiomers by Cyclodextrin Modified Capillary Zone Electrophohresis-Electrospray Ionization Mass Spectrometry." Journal of Chromatography A. 817. 75-81 (1998)

[Publications] 田中喜秀: "キャピラリー電気泳動/質量分析計による界面活性剤の分析" 分析化学. 47. 563-569 (1998)

[Publications] Joselito P.Quirino: "Exceeding 5000-fold Concentration of Dilute Analytes in Micellar Electrokinetic Chromatography" Science. 282. 465-468 (1998)

[Publications] Joselito P.Quirino: "Sample Stacking of Fast Moving Anions in Capillary Zone Electrophoresis under pH Suppressed Flow." Journal of Chromatography A. (印刷中). (1999)

[Publications] Joselito P.Quirino: "Sweeping of Analyte in Electrokinetic Chromatography." Analytical Chemistry. (印刷中). (1999)

[Publications] Joselito P.Quirino: "The Stacking Boundary in Capillary Electrophoresis." Journal of Microcolumn Separation. (印刷中). (1999)

[Publications] Joselito P. Quirino: "Sample concentration by Sample Stacking and Sweeping Using a Microemulsion and a Single-Isomer Sulfated β-Cyclodextrin as Pseudostationary Phases in Electrokinetic chromatography." Journal of Chromatography A. (印刷中). (1999)

[Publications] Koji Otsuka: "Recent Developments in MEKC-MS." Analusis. 26. M44-M47 (1998)

[Publications] Joselito P.Quirino: "Stacking of Neutral Analytes in Micellar Electrokinetic Chromatography." journal of Capillary Electrophoresis. 233-245 (1997)

[Publications] 尾崎祐人: "進歩総説 CE/MS" ぶんせき. 161-170 (1999)

寺部茂姫路工業大学, 理学部, 教授 (50115888)