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2000 Fiscal Year Final Research Report Summary

Molecular biological approach to improve the sink and source function in Rosaceae fruit tree

Research Project

Project/Area Number 09306002
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

YAMAKI Shohei  GRADUATE SCHOOL OF BIOAGRICULTURAL SCIENCES, NAGOYA UNIVERSITY, PROFESSOR, 大学院・生命農学研究科, 教授 (70210341)

Co-Investigator(Kenkyū-buntansha) SHIRATAKE Katsuhiro  GRADUATE SCHOOL OF BIOAGRICULTURAL SCIENCES, NAGOYA UNIVERSITY, ASSOCIATE PROFESSOR, 大学院・生命農学研究科, 助教授 (90303586)
KANAYAMA Yoshinori  TOHOKU UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE SCIENCES, ASSOCIATE PROFESSOR, 大学院・農学研究科, 助教授 (10233868)
Project Period (FY) 1997 – 2000
KeywordscDNA clone / sorbitol / sorbitol dehydrogenase / sorbitol-6-phospahte dehydrognase / sucrose synthase / fructokinase / H^+-pyrophosphatase / H^+-V-ATPase
Research Abstract

The purpose of this research is to make clear the sink-source relation of fruit by investigating the properties and gene expression of enzymes which play an important role for sugar metabolism and the vacuolar function to accumulate sugars.
1. Sorbitol dehydrogenase was purified from Japanese pear fruit, and its cDNA was also cloned from fruits of apple, loquat and peach. The gene expression was investigated throughout the development of their fruits.
2. The gene of sorbitol-6-phosphate dehydrogenase expressed on source leaves unfolded enough, but not on leaves folded yet.
3. Sucrsoe synthase isoform appearing with sugar accumulation was purified and the reaction inclined to sucrose synthesis rather than sucrose cleavage. The direction of reaction changes easily by phosphorylation and non-phosphorylation.
4. Two soluble acid invertases were purified from Japanese pear fruit, and there was no difference between properties of both isoforms.
5. We isolated the cDNA clone of fructokinase which is closely relating to fructose accumulation and examined the relations between the gene expression and fructose metabolism.
6. H^+-ATPase and H^+-pyrophosphatase which supply the energy needed to uptake sugars into vacuole were purified from pear fruit, and the gene expression throughout fruit development were investigated using cDNA clone of their subunits.
7. We could prove the presence of sugar transporters across the tonoplast by sugar uptake experiment, although could not isolate their protein and cDNA.
As described above, we could obtain the proteins and cDNA of some enzymes needed to accumulate sugars into fruit, analyzed their gene expressions and clarified partially the melecular mechanism of sugar accumulation.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kanayama,Y.: "Divergent frucutokinase genes are differentially expressed in to mato."Plant Physiol.. 113. 1379-1384 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiratake,K.: "Changes in H^+-pumps and a tonoplast intrinsic protein of vacuolar membranes during the development of pear fruit."Plant Cell Physiol.. 38. 1039-1045 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiratake,K.: "Characterization of hexose transporter for facilitated diffusion of the tonoplast vesicles from pear fruit."Plant Cell Physiol.. 38. 910-916 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanayama,Y.: "Tomato fructokinases exhibit differential expression and substrate regulation."Plant Physiol.. 117. 85-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,K.: "Cloning of NAD-dependent sorbitol dehydrogenase from apple fruit and gene expression."Plant Cell Physiol.. 39. 1375-1379 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakanishi,K.: "Expression of the gene for NADP-dependent sorbitol-6-phosphate dehydrogenase in peach leaves of various developmental stages."Plant Cell Physiol.. 39. 1372-1374 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,K.: "Gene expression of NAD-dependent sorbitol dehydrogenase during fruit development of apple (Malus pumila Mill.var.domestica Schneid.)."J.Japan.Soc.Hort.Sci.. 68. 1099-1103 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abnasan-Bantog,N.: "Changes in sugar content and sorbitol-and sucrose-related enzyme activities during development of loquat (Eriobotrya japonica Lindl.Mogi) fruit."J.Japan.Soc.Hort.Sci.. 68. 942-948 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,Y.: "Vacuolar H^+-pyrophosphatase purified from pear fruit."Phytochem.. 50. 535-539 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oura,Y.: "Purification and characterization of a NAD^+-dependent sorbitol dehydrogenase from Japanese pear."Phytochemistry. 54. 567-572 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abnasan-Bantog,N.: "Gene expression of NAD^+-dependent sorbitol dehydrogenase and NADP^+-dependent sorbitol-6-phosphate dehydrogenase during development of loquat (Eriobotrya japonica Lindl.) fruit."J.Japan.Soc.Hort.Sci.. 69. 231-236 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanase,K.: "Purification and characterization of two sucrose synthase isoformes from Japanese pear fruit."Plant Cell Physiol.. 41. 408-414 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiratake, K., Kanayama, Y.and Yamaki, S.: "Characterization of hexose transporter for facilitated diffusion of the tonoplast vesicles from pear fruit."Plant Cell Physiol. 38 (8). 910-916 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiratake, K., Kanayama, Y., Maeshima, M.and Yamaki, S.: "Changes in H^+-pumps and a tonoplast intrinsic protein of vacuolar membranes during the development of pear fruit."Plant Cell Physiol.. 38 (9). 1039-1045 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanayama, Y., Dai, N., Granot, D., Petreikov, M., Schaffer, A.and Bennett, A.B.: "Divergent frucutokinase genes are differentially expressed in tomato."Plant Physiol. 113. 1379-1384 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakanishi, K., Kanayama, Y., Mori, H., Yamada, K.and S.Yamaki: "Expression of the gene for NADP-dependent sorbitol-6-phosphate dehydrogenase in peach leaves of various developmental stages."Plant Cell Physiol. 39 (12). 1372-1374 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, K., Oura, Y., Mori, H.and Yamaki, S.: "Cloning of NAD-dependent sorbitol dehydrogenase from apple fruit and gene expression."Plant Cell Physiol.. 39 (12). 1375-1379 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanayama, Y., Granot, D., Dai, N., Petreikov, M., Schaffer, A., Powell, A.and Bennett, A.B.: "Tomato fructokinases exhibit differential expression and substrate regulation."Plant Physiol.. 117. 85-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, Y., Kanayama, Y., Shiratake, K.and Yamaki, S.: "Vacuolar H^+-pyrophosphatase purified from pear fruit."Phytochem.. 50 (4). 535-539 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abnasan-Bantog, N., Shiratake, K.and Yamaki, S.: "Changes in sugar content and sorbitol-and sucrose-related enzyme activities during development of loquat (Eriobotrya japonica Lindl. Mogi) fruit."J.Japan.Soc.Hort.Sci.. 68 (5). 942-948 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, K., Mori, H.and Yamaki, S.: "Gene expression of NAD-dependent sorbitol dehydrogenase during fruit development of apple (Malus pumila Mill. var. domestica Schneid.)."J.Japan.Soc.Hort.Sci.. 68 (6). 1099-1103 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanase, K.and Yamaki, S.: "Purification and characterization of two sucrose synthase isoforms from Japanese pear fruit."Plant Cell Physiol. 41 (4). 408-414 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bantog, N.A., Yamada, K., Shiratake, K.and Yamaki, S.: "Gene expression of NAD^+-dependent sorbitol dehydrogenase and NADP^+-dependent sorbitol-6-phosphate dehydrogenase during development of loquat (Eriobotrya japonica Lindl.) fruit."J.Japan.Soc.Hort.Sci.. 69 (3). 231-236 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oura, Y., Yamada, K., Shiratake, K.and Yamaki, S.: "Purification and characterization of a NAD^+-dependent sorbitol dehydrogenase from Japanese pear fruit."Phytochem.. 54. 567-572 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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