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2001 Fiscal Year Final Research Report Summary

Development of animal model for cancer researches concerning diet and nutrition using rasH2 mice

Research Project

Project/Area Number 09308033
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

MITSUOKA Tomotari  Graduate School of Agriculture and Life Sciences, The University of Tokyo, Honorary Prof., 大学院・農学生命科学研究科, 名誉教授 (30157549)

Co-Investigator(Kenkyū-buntansha) ITOH Toshio  Central Inst. of Exp. Anim., Group Director (Researcher), 実験動物中央研究所, 室長(研究職) (20106644)
NOMURA Tatsuji  Central Inst. of Exp. Anim., Head (Researcher), 実験動物中央研究所, 所長(研究職) (10072399)
ITOH Kikuji  Graduate School of Agriculture and Life Sciences, The University of Tokyo, Asosc. Prof., 大学院・農学生命科学研究科, 助教授 (50100045)
NAKAYAMA Hiroyuki  Graduate School of Agriculture and Life Sciences, The University of Tokyo, Associate Prof., 大学院・農学生命科学研究科, 助教授 (40155891)
HIOKI Kyouji  Central Inst. of Exp. Anim., Group Director (Researcher), 実験動物中央研究所, 室長(研究職) (80208735)
Project Period (FY) 1997 – 2000
KeywordsCancer / ras mice / transgenic mice / diet / Fermentation products of lactic acid bacteria
Research Abstract

1. To study the effects of intestinal flora on cancer development, N-methyl-nitrosourea (MNU) and N,N-diethylnitrosoamine (DEN), which induce the mutations in c-Ha-ras genes, were injected to germfree and various types of gnotobiote rasH2 mice. There were no relation between cancer development and the composition of intestinal flora.
2. Although rasH2 mice were inoculated with Aαc, one of heterocyclic amines, and aflatoxin, which are strong carcinogens and ingested from the daily diet, no cancers were observed.
3. Inhibitory effect of various functional foods was studied using rasH2 mice injected with 1,2-dimethylhydrazine (DMH). Apple pectin and culture condensate of Bifidobacterium longum inhibited colorectal tumors. However there were no relation between development of colorectal tumors and the intestinal environments, e.g. composition of intestinal flora, concentrations of organic acids and putrefactive products, composition of bile acids.
4. Culture of Propionibacterium freudereichii, which is used as starter of cheese and is immunomodulator, did not inhibit the colorectal tumors in rasH2 mice induced by DMH. Yogurt, fermented milk by Streptococcus thermophilus and Lactobacillus burgaricus, reduced the incidence of the colorectal tumors in rasH2 mice induced by DMH. Fermented soy bean protein by single culture of Lactobacillus casei, L. fermentum, Saccharomyces cerevisiae or mixed culture of them could inhibited the colorectal tumors, but not fermented protein by Leuconostoc mesenteroides and mixed culture of this with other three strains.
These results indicate that rasH2 mice are good animal model to evaluate the inhibitory effects of functional food on the colorectal tumors.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Narushima, S.他: "Effect of mouse intestinal bacteria on incidence of colorectal tumors laduced by 1,2-dimethyl-hydnazine injection in gnotopiotic transgenic mice hanboring human prototype c-Ha-ras genes"Exp. Anim.. 47. 111-117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, S.他: "Validation of thansgenic mice hanboring the human prototype C-Ha-ras gene as a bioassay model for rapid canciaogenicity testing"Toxicol. Lett.. 102-103. 473-478 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohno, K.他: "Inhibitory effect of apple pectin and culture condensate of Bifidobacterium longum on colorectal tumors induced by 1,2-dimethylhydnezine in transgenic mice hanboring human"Exp. Anim.. 49. 305-307 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohno, K.他: "Effect of bacterial metabolisan in the intestine on colonectal tumors induced by 1,2-dimethylhydnezine in transgenic mice harboring human prototype c-Ha-ras genes"J. Exp. Clin. Cancer Res.. 20. 51-56 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Birumachi, J.他: "Diesel exhaust-induced airway hypernesponsiveness in c-Ha-ras transgenic mice"Toxicol.. 163. 145-152 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umemura, T.他: "Buthylhydrixy to luene (BHT) increases susceptibility of transgenic ras H2 mice to lung cancinogenesis"J. Cancer Res. Clim. Oncol. 127. 583-590 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Narushima, S. et al.: "Effect of mouse intestinal bacteria on incidence of colorectal tumors induced by 1,2-dimethylhydrazine injection in gnotobiotic transgenic mice harboring human prototype c-Ha-ras genes."Exp. Anim.. 47. 111-117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, S. et el.: "Varidation of transgenic mice harboring the human prototype c-Ha-ras gene as a bioassay model for rapid carcinogenicity testing"Toxicol. Lett.. 102-103. 473-478 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohno, K. et al.: "Inhibitory effect of apple pectin and culture condensate of Bifidobacterium longum on colorectal tumors induced by 1,2-dimethylhydrazine in transgenic mice harboring human. prototype c-Ha-ras genes"Exp. Anim.. 49. 305-307 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohno, K. et al.: "Effect of bacterial metabolism in the intestine on colorectal tumors induced by 1,2 -dimethylhydrazine in transgenic mice harboring human prototype c-Ha-ras genes"J. Exp. Clin. Cancer Res.. 20. 51-56 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Birumachi, J. et al.: "Diesel exhaust-induced air way hyperresponsiveness in c-Ha-ras transgenic mice"Toxicol. 145-152 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Umemura, T. et al.: "Buthylhydrixy to luene (BHT) increases susceptibility of transgenic rasH2 mice to lung carcinogenesis"J. Cancer Res Clin. Oncol.. 127. 583-590

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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