Malaria transmission blocking with reference to gametocyte

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 09309010
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 広領域
• Research Institution: Jichi medical School
• Principal Investigator: ISII Akira Jichi Medical School, Department of Medical Zoology, Prof., 医学部, 教授 (40012752)
• Co-Investigator(Kenkyū-buntansha): CHINZEI Yasuo Mie University, School of Medicine, Department of Medical Zoology, Prof., 医学部, 教授 (60024709) ; KAWAMOTO Fumihiko Nagoya University, School of Medicine, Department of International Health, Ass.Prof., 医学部, 助教授 (40115556) ; MATSUOKA Hiroyuki Jichi Medical School, Department of Medical Zoology, Ass.Prof., 医学部, 助教授 (10173816) ; SUGURI Setsuo Kagawa Medical University, Department of Medical Zoology, Ass.Prof., 医学部, 助教授 (00032897) ; KAWABATA Masato Kobe University, School of Medicine, International Medical Center.Prof., 医学部, 教授 (30175294)
• Project Period (FY): 1997 – 1999
• Keywords: malaria / gametocyte / malaria control / epidemiology / primaquine / G6PD / TRAP / GIS

Research Abstract

Sporozoites were found to enter in the intestinal system. Sporozoites were demonstrated in prediuresis fluid. Changes in salivary gland components after the feeding process were chased and a salivary gland specific band was cloned. It has 30% homology with a allergen from salivary gland of Aedes aegypti, Detection of sporozoites in mosquito was made possible by the use of PCR. Gametocyte formation was found to be enhanced with increase of hamatocrit in culture. Superoxide was found to affect oocyst formation in P47 knockout mice. Chitinase was found to play roles in oocyst formation. A adhesion molecule on ookinate surface was found crucial on invasion into the midgut. IgG subclass found to differ in host immune reactions to malaria antigen. Some more vaccine candidate molecules such as neutrophil chemotactic factor were investigated with a hope of transmission blocking. Chemotherapeutic malaria control was attempted with simple diagnostic kit and G6PD deficiency detection kit (SSS -system). Chemotherapy was done for blood positives with chloroquine and primaquine to kill gametocytes. This was proved to be effective in the field and promising in computer simulation with a mathematical epidemiology model. GIS was applied to analyze transmission capability of the field.

Research Products

• [Publications] Tantular.S., et al: "Field trials of a rapid test for G6PD deficieny in combination with a rapid diagnosis of malaria."Trop.Med.Internat. Hlth.. 4(4). 245-250 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ishii,A., et al: "Chemotherapeutic malaria control as a slelective primary bealth care activity with cost analysis in the Solomon Islands."Parassitologia. 41(1/3). 383-384 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] S.Yoshida, et al: "A single-chain antibody fragment specific for the Plasmodium berghei ookinate protein Pbs21 confers transmission blockade in the mosquito midgut."Mol.Biochem.Parasitol.,. 104(2). 195-204 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石井明、他: "選択的プライマリーヘルスケアにおけるマラリア対策方法の検討:ソロモン諸島における治療対策の試みと費用分析。"国際保健医療. (印刷中). (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fu,J., et al: "A simple screening method detecting bindings between oligopeptides and HLA-DR molecules on filter papers."Microbiol.Immunol.. (In press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Luo,E, et al: "Changes of salivary proteins during feeding and detection of salivary proteins in the midg after feeding in a malaria vector mosquito,Anopheles stephensi (Diptera:Culicidae)."Med.Ent.Zool.,. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tantular,I.S., et al: "Field survey of malaria the Flores island,East Nusa Tenggara Province,Indonesia, and detection of Plasmodium malariae and P.ovale using two PCR-based diagnoses."Folia Medica Indonesia,. 35. 38-42 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Da Silveria,L.A., et al: "Allelic diversity and antibody recognition of Plasmodium falciparum merozoite surface protein l during hypoendemic malaria transmission in the Brazilian Amazon region."Infection & Immunity,. 67. 5906-5916 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 川本文彦、他: "マラリア原虫を見落とすな-アクリジンオレンジ染色。"臨床検査. 43. 1625-1629 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kawamoto,F., et al: "How prevalent are Plasmodium ovale and P.malariae in east Asia?"Parasitol.Today. 15. 422-426 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fu J, et al: "Epitope-specific impairment of production of antibody against merozoite sueface glycoprotein 1 of Plasmodium falciparum in symptomatic patients with malaria."Parasitology Research,. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fu J, et al: "A simple screening method detecting bindings between oligopeptides and HLA-DR molecules on filter papers:Possible application for mapping of putative helper T cell epitopes on MSP1 of Plasmodium falciparum."Microbiology and Immunology,. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Yuda, et al: "Structure and expression of an adhesive protein-like molecule of mosquito invasive-stage malaria parasite."J.Exp.Med.. 189. 1947-1952 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Yuda, et al: "Targetted disruption of the Plasmodium berghei CTRP gene reveals its essential role in malaria infection of the vector mosquito."J.Exp.Med.. 190. 1711-1715 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石井明、他: "マラリア学100年会議(Malariology Centenary Conference)"医学のあゆみ. 188(8). 806-811 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石井明: "世界保健機構(WHO)の新しい国際寄生虫病対策戦略 特集:地球規模での寄生虫病対策の時代"医学のあゆみ. 191(1). 3-6 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石井明: "マラリア対策と日本の協力,国際医療協力を考える"第25回医学会総会 会誌III. III. 382 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石井明: "4類感染症の診断と治療の進め方,マラリア"Medical Practice. 16(11). 1813-1816 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] A.Ishii,N.Nihei & M.Sasa(ed): "Malaria Reseasch in the Solomon Jslands."Inter Group.. 192 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 石井明、鎮西康雄、太田伸生: "標準医動物学"医学書院. 309 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fu, M. Kunimitsu, J. L. Leafasia, N. Kere, K. Tanabe, K. Hirayama, Ishii, A. Saitoh-Ito, M. Sasaki and N. Ohta: "MSPI-reactive T cells in individuals in malaria Solomon area and in non-immune Japanese"Parasitology International. 46(1). 7-16 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] C. M. Rzepczyk, K. Hale, N. Woodroffe, A. Bobogare, P. Csurhes, A. Ishii and A. Ferrante: "Humoral immune responses of Solomon Isalnders to the merozoite surface antigen 2(MSA2, MSP2) of Plasmodium falciparum show pronounced skewing towards anitibodies of the Ig G3 subclass"Infect.Immun.. 65(3). 1098-1100 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsuok, H., H. Matsubara, P. Waidhet, T. Hashiimoto, A. Ishii, Y. Saito, K. Ando and Y. Chinzei: "Depletion of salivary gland proteins in Anopheles stephensi (Diptera:culicidae) on blood feeding, and induction of antibodies to the proteins in mice being fed"Med.Entomol.Zool.. 48(3). 211-218 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakazawa, M., H. Ohmae, A. Ishii, J. Leafasia: "Malaria infection and human behavioral factors : A stochastic model analysis for direct observation data in the Solomon Islands"Am.J.Human Biol.. 10. 781-789 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] A. Yoshida, A. Ishii, J. L. Leafasia, D. Zhou, E. Chen, L. Tang, C. Lie, D. Qiu, H. Ohmae and N. Ohta: "Polymorphism of Tumor Necrosis Factor α gene in the Melanesian and East-Asian populations"Immunogenetics. 47(6). 497-498 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tantular. S., K. Iwai, Klin, S. Basuki, T. Horie, H. H. Htay, H. Matsuoka, H. Marwoto, C. Wongsrichanalai, Y. O. Dachlan, S. Kojima, A. Ishii & F. Kawamoto: "Field trials of a rapid test for G6PD deficieny in combination with a rapid diagnosis of malaria"Trop.Med.Internat.Hlth.. 4(4). 245-250 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishii, A., N. Nagai, M. Arai, M. Kawabata, T. Matsuo, A. Bobogare & J. Leafasia: "Chemotherapeutic malaria control as a slelective primary health care activity with cost analusis in the Solomon Islands"Parassitologia. 41(1/3). 383-384 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] S. Yoshida, H. Matsuoka, E. Luo, K. Iwai, M. Arai, R. E. Sinden and A. Ishii: "A single-chain antibody fragment specific for the Plasmodium berghei ookinate protein Pbs21 confers transmission blockade in the mosquito midgut"Mol.Biochem.Parasitol.. 104(2). 195-204 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ishikawa, H., A. Ishii and A. Kaneko: "The prevalence of Plasmodium vivax in Vanuatu Islands : Computer simulation of malaria control trials"J.Faculty Environmental Science and Technology, Okayama University. 5(1). 1-6 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fu, J., Hato, M., Igarashi, K., Suzuki, T., Matsuoka, H., H., Ishii, A., Leafasia, J., Chinzei, Y., Ohta, N.: "A simple screening method detecting bindings between oligopeptides and HLA-DR molecules on filter papers"Microbiol.Immunol.. (In press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Luo, E., H. Matsuoka, D. Yoshida, K. Iwai, M. Arai and A. Ishii: "Changes of salivary proteins during feeding and detection of salivary proteins in the midgut after feeding in a malaria vector mosquito, Anopheles stephensi (Diptera:Culicidae )"Med.Ent.Zool.. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Akira Ishii: "Medical Information on the Solomon Islands p17-18. Ishii, Nihei & Sasa(ed.) "Malaria Research In The Solomon Islands""Inter Group Co., Tokyo. 192 (1998)
  • Description: 「研究成果報告書概要(欧文)」より