• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Bio-Cell Gene Expression Analyzer

Research Project

Project/Area Number 09355027
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUOKA Hideaki  Tokyo Univ.Agricul. Technol., Dept.Technol., Professor, 工学部, 教授 (10143653)

Co-Investigator(Kenkyū-buntansha) SAITO Mikako  Tokyo Univ. Agricul. Technol., Dept. Technol., Research Assistant, 工学部, 講師 (20291346)
OH Ki-bong  Tokyo Univ. Agricul. Technol., Dept. Technol., Assistant Professor (90272632)
NEMOTO Yasuyuki  Tokyo Univ. Agricul. Technol., Dept. Technol., Assistant Professor (70202249)
KAWANA Akio  NTT Basic Research Laboratories, Material Science Research Laboratory, Kawana Research Group Leader
Project Period (FY) 1997 – 2000
KeywordsBio-Cell / gene expression / stress signal
Research Abstract

The term "bio-cell" means a cell that maintains its viable state throughout the experiments of stress application. This study was done in order to demonstrate the real time analysis of the bio-cell responses to various stress signals. An electric stress signal was applied to cultured cells of tobacco or rice. As the cell response, the gene expression was measured with a microscopic fluorescent image analyzing system. By the experiment using tobacco cells, line BY-2, the electric impedance of the cell membrane was measured under various intensities of pulsing electric stress signals. As the result, appreciable membrane permeabilization was observed when the cross membrane potential (V_<CMP>) exceeded 200mV.On the other hand, the Ca^<2+>-dependent expression of chitinase gene was demonstrated by 2-step experiments using rice protoplasts. At the first step, Ca^<2+> introduction was controlled by the pulsing electric stress signal. As the result, Ca^<2+> entered the cells around 1-5 mV of VCMP.As the 2nd step, we demonstrated the chitinase gene expression by the direct injection of Ca^<2+> into the protoplasts. Besides the electric stress, we intended to apply an electrochemical stress to a bio-cell at its inside. Then we developed a capillary carbon microelectrode with a 1-2 μm diameter and checked its performance by analyzing cyclicvoltammograms of dopamine. In conclusion, the analyzing system of the gene expression of a bio-cell was developed and its promising performance was demonstrated.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Mikako Saito: "pH microelectrode-micropipette system for the measurement of enzyme reactions in a picoliter volume of a living plant cell"Anal.Chim.Acta. 404(2). 223-229 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikako Saito: "Elicitor action via cell membrane of a cultured rice cell demonstrated by the single-cell transient assay"J.Biotechnol.. 76(2/3). 227-232 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikako Saito: "Modulation of Ca^<2+> influx into a single rice cell with an electric signal"Electrochemistry. 68(5). 333-336 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikako Saito: "Use of acetylene for the fabrication of a glass capillary carbon microelectrode"Electrochemistry. 68(11). 924-926 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Matsuoka: "Microbioelectronics for single-cell experiment"Electrochemistry. 68(5). 314-320 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 斉藤美佳子: "ボーダーレス電気化学者をめざす"電気化学. 69(2). 128-129 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 斉藤美佳子: "植物を利用するセンサ技術(Sensor technology using plants)",バイオミメティックスハンドブック(長田義仁編集)機能応用編第9章個体系5節3"エヌ・ティー・エス. 6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hidekazu Sotoyama, Mikako Saito, Ki-Bong Oh, Yasuyuki Nemoto and Hideaki Matsuoka: "In vivo measurement of the electrical impedance of cell membranes of tobacco cultured cells with a multifunctional microelectrode system"Bioelectrochem. Bioenerg.. 45(1). 83-91 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka, Akio Ohi, Hidekazu Sotoyama and Mikako Saito: "Use of a microelectrode system as a tool of the quantitative application of wound to the cell-to-cell junctional membrane of cultured tobacco cells"Denki Kagaku. 66(5). 532-536 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka, Taichiro Sugisaki, Hiroko Ishizaka, Mikako Saito and Ki-Bong Oh: "Sensitive and real time measurement of root growth by a computer-aided image analyzing system"Commun. Soil Sci. Plant Anal.. 29(9/10). 1341-1348 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka, Tatsuya Kotani, Mikako Saito and Ki-Bong Oh: "Feasibility of a radicle of Brassica campestris L.as a bio-indicator of plant growth inhibitors"J.Biotechnol.. 62(3). 187-193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidekazu Sotoyama, Mikako Saito, Ki-Bong Oh and Hideaki Matsuoka: "Control of the cross membrane potential of a cultured tobacco cell and simultaneous measurement of the electrical impedance of its cell membrane"Electrochemistry. 67(1). 18-21 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka, Mikako Saito, Toshihiko Watabe, Akio Ohi, Hidekazu Sotoyama and Ki-Bong Oh: "Control of the quantity and the timing of microinjection into a bio-cell with a multifunctional microelectrode"Electrochim. Acta.. 44. 3801-3807 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka, Hidekazu Sotoyama, Mikako Saito, Ki-Bong Oh and Shigetoshi Horikiri: "Effects of a pulsing electric signal on the cross membrane potential and the cell division potentiality of a single cell tobacco"Bioelectrochem. Bioenerg.. 49(1). 65-72 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito and Hideaki Matsuoka: "pH microelectrode-micropipette system for the measurement of enzyme reactions in a picoliter volume of a living plant cell"Anal.Chim.Acta. 404(2). 223-229 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito, Takashi Chikazawa, Hideaki Matsuoka, Yoko Nishizawa and Naoto Shibuya.: "Elicitor action via cell membrane of a cultured rice cell demonstrated by the single-cell transient assay"J.Biotechnol.. 76(2/3). 227-232 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhiro Iida, Ki-Bong Oh, Mikako Saito, Hideaki Matsuoka and Hiroshi Kurata: "In vitro synergism between nyasol, an active compound isolated from Anemarrhena asphodeloides, and azole agents against Candida albicans"Planta Med.. 66. 435-438 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito, Yuri Kosai and Hideaki Matsuoka: "Modulation of Ca^<2+> influx into a single rice cell with an electric signal"Electrochemistry. 68(5). 333-336 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuya Yamada, Masanori Nakata, Naoki Horimoto, Mikako Saito, Hideaki Matsuoka and Nobuya Inagaki: "Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic β-cells"J.Biol.Chem.. 275(29). 22278-22283 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito, Ayako Saga, Hideaki Matsuoka and Danny K.Y.Wong: "Use of acetylene for the fabrication of a glass capillary carbon microelectrode"Electrochemistry. 68(11). 924-926 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito, Hideaki Matsuoka: "Measurement of plant bio-cell--towards the elucidation of systemic response mechanism in plant"Tanpakushitsu Kakusan Kouso. 42(5). 784-789 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka and Mikako Saito: "Microbioelectronics for single-cell experiment"Electrochemistry. 68(5). 314-320 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikako Saito, Hideaki Matsuoka: ""Sensor technology using plants" Biomimetics handbook"NTS. 1038-1043 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi