1999 Fiscal Year Final Research Report Summary
Development and analysis of secretory component disrupted mouse
| Project/Area Number |
09357016
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
MORO Itaru Nihon University, Professor, 歯学部, 教授 (50059531)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kahei Nihon University, Professor, 生物資源科学部, 教授 (20059778)
OHKUBO Nobuko (竹之内 信子) Nihon University, Instructor, 歯学部, 助手 (50246914)
IWASE Takashi Nihon University, Assistant professor, 歯学部, 講師 (80125046)
KAI Osamu Nihon University, Associate professor, 生物資源科学部, 助教授 (90115543)
ASANO Masatake Nihon University, Instructor (10231896)
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| Project Period (FY) |
1997 – 1999
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| Keywords | secretory component / mucosal immunity / gene / mRNA / genomic DNA / gene targeting / polymeric IgA / S-IgA |
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| Research Abstract |
Secretory component (SC), often called polymeric immunoglobulin receptor (pIgR), is a component of SIgA which plays an important role in the mucosal defense system. SC which is localized on the basolateral membrane of the epithelial cells at the mucosal site is a receptor for polymeric immunoglobulins. Accordingly, it transports polymeric immunoglobulin and immune complex through epithelial cells into the secretion. SC is a transmembrane protein with an Mr of 80 kDa abd cDNA revealed 1 lexons composed of extracellular region, transmembrane region and cytoplasmic tail.
The purpose of this project is to develop SC disrupted mouse to examine the function of SC.
The results obtained were as follows;
1) genomic DNA for SC was screened using a mouse pIgR cDNA and was determined by sequencing of positive clones.
2) Five positive clones were obtained. The results of restriction enzyme map and Southern blotting revealed a 9.4 kb of these clones. Sequencing analysis indicated that the BamH I-EcoR I fragment was 8404bp, and exon 4, 5, 6, 7, 8, 9, 10 were 653 bp, 352 bp, 332 bp, 192 bp, 129 bp, 131 bp and 58 bp, respectively. Introns 4, 5, 6, 7, 8, 9, 10 were 1237 bp, 552 bp, 891 bp, 359 bp, 368 bp, 1106 bp and 1010 bp, respectively. The size of the exon 11 to EcoR I fragment was 2543 bp.
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