1999 Fiscal Year Final Research Report Summary
Regulation of expression of photosynthetic genes by the two-component regulatory system of photosynthetic bacteria
| Project/Area Number |
09440265
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TAKAMIYA Ken-ichiro Faculty of Bioscience and Biotechnology, Professor, 生命理工学部, 教授 (80037259)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Hiroshi Faculty of Bioscience and Biotechnology, Associate Professor, 生命理工学部, 助手 (80301175)
MASUDA Tatsuru Faculty of Bioscience and Biotechnology, Associate Professor, 生命理工学部, 助手 (00242305)
OHTA Hiroyuki Faculty of Bioscience and Biotechnology, Associate Professor, 生命理工学部, 助教授 (20233140)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Photosynthetic gene / Photosynthetic bacteria / Two-component regulatory system / bactriochlorophyll / signal transduction / Oxygen / Light |
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| Research Abstract |
1. Genes downstream of the two-component regulatory system of Rhodobacter sphaeroides were cloned and they were spb, orf 5, orf 318 and ahcY.
2. Spb was a homologue of hvrA in Rhodobacter capsulatus but its function was differed from that of Rba. capsulatus in that spb was light-repressor, whereas hvrA was light-activator
3. Orf 318 was a novel gene which had homology with RfaI and RfaJ, which are glycosyltransferases involved in the synthesis of lipopolysaccharide in bacteria.
4. Disruption of orf 5 caused inhibition of growth rate in the dark under aerobic conditions.
5. In an aerobic photosynthetic bacterium, Roseobacter denitrificans, there existed similar genes including two-component regulatory genes, indicating that the regulatory system for photosynthetic genes was essentially the same in these bacteria.
6. The results obtained indicate the possible involvement of genes downstream of the two-component regulatory gene in the signal transduction pathway.
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[Publications] Mizoguchi, H., Masuda, T., Nishimura, K, Shimada, H., Ohta, H., Shioi, Y, and Takamiya, K.: "Nucleotide sequence and transcriptional analysis of the flanking region of the gene(spb) for the trans-acting factor that controls light-mediated expression of the puf operon in Rhodobacter sphaerodes"Plant Cell Physiol.. 38(5). 558-567 (1997)
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[Publications] Nishimura, K, Shimada, H., Hatanaka, S., Mizoguchi, H., Ohta, H., Masuda, T.and Takamiya, K.: "Growth, pigmentation, and expression of the puf and puc operons in a light-responding-repressor (SPB)-disrupted Rhodobacter sphaeroides"Plant Cell Physiol.. 39(4). 411-417 (1998)
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[Publications] Obayashi, T., T., Shimada, H., Nishimura, K. Masuda, T., Ohta, H. and Takamiya, K.: "Properties of orf5-disrupted and overexpressed Rhodobacter sphaeroides mutants"In Photosynthesis : Mechanisms and Effects, Ed. G. Garab, Vol. IV, 2893-2896, Kluwer Academic Publishers, The Netherlands. (1998)
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[Publications] Masuda, T., Nagayama, M., Inoue, K, Shimada, H., Ohta, H., and Takamiya, K.: "Analysis of bacteriochlorophylls in zinc-containing bacteriochlorophyll producing aerobic bacterium, Acidiphilium rubrum, by high-performance liquid chromatography"In Photosynthesis : Mechanisms and Effects, Ed. G. Garab, Vol. IV, 3233-3236, Kluwer Academic Publishers, The Netherlands. (1998)
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[Publications] Masuda, T., T., Inoue, K, Masuda, Nagayama, M., Tamaki, A., Ohta, H., Shimada, H., & Takamiya, K.: "Magnesium insertion by magnesium chelatase in the biosynthsis of zinc-bacteriochlorophyll a in an aerobic acidophilic bacterium Acidiphilium rubrum"J. Biol. Chem.. 274(47). 33594-33600 (1999)
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[Publications] Nishimura, K., Shimada, H., Shinmen, T., Obayashi, T., Masuda, T., Ohta, H., and Takamiya, K.: "Photosynthetic regulatory gene cluster in and aerobic photosynthetic bacterium, Roseobacter denitrificans"J. Gen. Appl. Microbiol.. 45(1). 129-134 (1999)
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