1999 Fiscal Year Annual Research Report

テトラヒメナ・クエン酸合成酵素の多機能性の研究

Research Project

Project/Area Number	09440275
Research Institution	University of Tsukuba
Principal Investigator	沼田治筑波大学, 生物科学系, 助教授 (50189354)
Keywords	テトラヒメナ / クエン酸合成酵素 / ミトコンドリア / 細胞骨格 / 多機能性 / hsp60 / hsp70 / シャペロン
Research Abstract	テトラヒメナ・クエン酸合成酵素は細胞質では直径14nmの繊維を形成し、細胞骨格として機能し、ミトコンドリアではクエン酸合成酵素として機能している。本研究はクエン酸合成酵素の多機能性がどのように調節されているかを明らかにすることを目的とする。クエン酸合成酵素の多機能性を調節している因子の同定を試みるため、大腸菌で発現させたリコンビナントクエン酸合成酵素を用いて、クエン酸合成酵素のアフィニティーカラムを作成した。このカラムに細胞抽出液をのせ、分子量70kDa,60kDaのクエン酸合成酵素結合蛋白質を見い出した。これらの蛋白質のN末アミノ酸配列を決定した結果、それぞれミトコンドリアに存在するhsp70とhsp60であることが判った。hsp60の抗体を用いてクエン酸合成酵素と局在性を比較した結果、両者ともミトコンドリアと口部装置に存在することが判った。今後これらの蛋白質とクエン酸合成酵素の多機能性の関係に関して検討する予定である。

Research Products

(10 results)

All Other

All Publications (10 results)

[Publications] Numata.O.,Kurasawa,H.,and Watanabe.Y.: "Tetrahymena elongation factor-1 α binds to Hsp70 family proteins."Zoological Science. 16. 279-284 (1999)
[Publications] Fujiu,K.,and Numata,O.: "Localization of microtubules during macronuclear division in Tetrahymena and possible involvement of macronuclear microtubules in 'amitotical' chromatin distribution."Cell Structure and Function. 24. 401-404 (1999)
[Publications] Gonda,K.,Nishibori,K.,Ohba,H.,Watanabe,A.,and Numata,O.: "Molecular cloning of the gene for p85 that regulates the initiation of Cytokinesis in Tetrahymena."Biochemical and Biophysical Research Communication. 246. 112-118 (1999)
[Publications] Gonda,K.,Katoh,M.,Hanyu,K.,Watanabe,Y.,and Numata,O.: "Ca^<2+>/calmodulin and p85 cooperatively regulate an initiation of cytokineses in Tetrahymena."Journal of Cell Science. 112. 3619-3626 (1999)
[Publications] Wang,S.,Nakashima,S.,Numata.O.,Fujiu,K.,and Nozawa,Y.: "Molecular cloning and cell-cycle-dependent expression of the acetyl-CoA synthetase gene in Tetrahymena cells."Biochem J.. 343. 479-485 (1999)
[Publications] Numata.O.,Kurasawa,H.,Gonda,K.,and Watanae.Y.: "Tetrahymena elongation factor-1 α is localized with calmodulin in the division furrow."Journal of Biochemistry. 127. 51-56 (2000)
[Publications] Watanabe,A.,Yonemura,I.,Gonda,K.,and Numata,O.: "Cloning and sequencing of the gene for a Tetrahymena fimbrin-like protein."Journal of Biochmistry. 127. 85-94 (2000)
[Publications] Numata,O.,Gonda,K.,Watanabe,A.,and Kurasawa,Y.: "Cytokinesis in Tetrahymena: determination of division plane and organization of contractile ring."Microsco.Res.Tech.. (in press). (2000)
[Publications] Numata,O.,Fujiu,K.and Gonda,K.: "Macronuclear division and cytokinesis in Tetrahymena."Cell Biol.Int.. (in press). (2000)
[Publications] Numata, O., K. Takeda, T., Watanabe, Y.: "Tetrahymena callcium-binding proteins, TCBP-23 and TCBP25"In Tetrahymena thermophia, ed. By D.J. Asai and J.D. Forney, Methods in Cell Biology, 62巻, Academic Press. 11 (1999)

1999 Fiscal Year Annual Research Report

テトラヒメナ・クエン酸合成酵素の多機能性の研究

Principal Investigator

沼田 治 筑波大学, 生物科学系, 助教授 (50189354)

Research Products

[Publications] Numata.O.,Kurasawa,H.,and Watanabe.Y.: "Tetrahymena elongation factor-1 α binds to Hsp70 family proteins."Zoological Science. 16. 279-284 (1999)

[Publications] Fujiu,K.,and Numata,O.: "Localization of microtubules during macronuclear division in Tetrahymena and possible involvement of macronuclear microtubules in 'amitotical' chromatin distribution."Cell Structure and Function. 24. 401-404 (1999)

[Publications] Gonda,K.,Nishibori,K.,Ohba,H.,Watanabe,A.,and Numata,O.: "Molecular cloning of the gene for p85 that regulates the initiation of Cytokinesis in Tetrahymena."Biochemical and Biophysical Research Communication. 246. 112-118 (1999)

[Publications] Gonda,K.,Katoh,M.,Hanyu,K.,Watanabe,Y.,and Numata,O.: "Ca^<2+>/calmodulin and p85 cooperatively regulate an initiation of cytokineses in Tetrahymena."Journal of Cell Science. 112. 3619-3626 (1999)

[Publications] Wang,S.,Nakashima,S.,Numata.O.,Fujiu,K.,and Nozawa,Y.: "Molecular cloning and cell-cycle-dependent expression of the acetyl-CoA synthetase gene in Tetrahymena cells."Biochem J.. 343. 479-485 (1999)

[Publications] Numata.O.,Kurasawa,H.,Gonda,K.,and Watanae.Y.: "Tetrahymena elongation factor-1 α is localized with calmodulin in the division furrow."Journal of Biochemistry. 127. 51-56 (2000)

[Publications] Watanabe,A.,Yonemura,I.,Gonda,K.,and Numata,O.: "Cloning and sequencing of the gene for a Tetrahymena fimbrin-like protein."Journal of Biochmistry. 127. 85-94 (2000)

[Publications] Numata,O.,Gonda,K.,Watanabe,A.,and Kurasawa,Y.: "Cytokinesis in Tetrahymena: determination of division plane and organization of contractile ring."Microsco.Res.Tech.. (in press). (2000)

[Publications] Numata,O.,Fujiu,K.and Gonda,K.: "Macronuclear division and cytokinesis in Tetrahymena."Cell Biol.Int.. (in press). (2000)

[Publications] Numata, O., K. Takeda, T., Watanabe, Y.: "Tetrahymena callcium-binding proteins, TCBP-23 and TCBP25"In Tetrahymena thermophia, ed. By D.J. Asai and J.D. Forney, Methods in Cell Biology, 62巻, Academic Press. 11 (1999)

沼田治筑波大学, 生物科学系, 助教授 (50189354)