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1998 Fiscal Year Final Research Report Summary

Physiochemical Study on Highly Selective Accumulation of Vanadium Ions by Ascidians

Research Project

Project/Area Number 09440278
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MICHIBATA Hitoshi  Hiroshima University, Faculty of Science, Professor, 理学部, 教授 (00111740)

Co-Investigator(Kenkyū-buntansha) UEKI Tatsuya  Hioshima Univesity, Faculty of Science, Assistant Professor, 理学部, 助手 (10274705)
UYAMA Taro  Hioshima Univesity, Faculty of Science, Lecturer, 理学部, 講師 (60232914)
KANAMORI Kan  Toyama University, Faculty of Science, Professor, 理学部, 教授 (00019001)
Project Period (FY) 1997 – 1998
KeywordsProtochordates / Metal Accumulation / Redox / Vanadium / Vacuole / pH / Recombinant Proteins / Monoclonal Antibody
Research Abstract

A lot is known about the vanadium in ascidians, which are also known as tunicates or sea squirts. For example, the concentration of vanadium attains 350 mMin the blood cells of Ascidia gemmata belonging to the suborder Phlebobranchia, which is l0^7times higher than the concentration in sea water among approximately ten types of blood. cells, signet ring cells are true vanadocytes, vanadium-containing blood cells, almost all vanadium is reduced to the +3 oxidation state (V^<III>) via the +4 oxidation state (V^<IV>) and stored in vacuoles of vanadocytes, although vanadium is in the +5 oxidation state (V^V) in seawater.
We have revealed by the research project followings : a vanadium-associated protein (VAP) extracted from the blood cells of A.sydneiensis sames was revealed to contain 16 atoms of vanadium per a mol ; the same antigen recognized with a polyclonal antibody against VAP was detected in the precipitated proteins of the polychaete Pseudopotamilla occelata which was the first animal revealed to contain high levels of vanadium besides ascidians ; apo-VAP, VAP removed vanadium, was found to bind 10 atoms of vanadium ; the nucleotide sequences of cDNAs encoding 12.5 kDa and 15 kDa of VAPs are revealed ; a fusion protein was isolated from E.coil transfected with plasmid vector expressing VAPs genes ; a monoclonal antibody specific to vacuolar membrane of vanadocytes was prepared ; G6PDH, 6PGDH, and transketolase in the pentose phosphate pathway and glycogen phosphorylase were found to be in the cytoplasm of vanadocytes ; NADPH was revealed to reduce V^V-EDTA to V^<IV>-EDTA.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Michibata,H.: "Vanadocyte is a cell holding the key to resolve the highly selective accumulation and reduction of vanadium in ascidians." Microscop.Res.Technol.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michibata,H.: "The accumulation mechanism of vanadium by ascidians- An Interdisciplinary study between biology and chemistry on extraordinary high levels and reduced form of vanadium in vanadocytes." Am.Chem.Soc.Symp.Ser.711. 248-258 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michibata,H.: "Selective accumulation of vanadium by ascidians from seawater." Adv.Environm.Sci.&Technol.edit.Nriagu J.John Wiley&Sons,Inc.217-249 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueki,T.: "Isolation of cDNAs encoding subunits A and B of the vacuolar-type ATPase from the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.15. 823-829 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama,T.: "A 100-kDa antigen recognized by a newly prepared monoclonal antibody specific to the vanadocytes of the vanadium-rich ascidian,Ascidia sydneiensis samea,is glycogen phosphorvlase." Zool.Sci.15. 815-821 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama,T.: "Glucose-6-phosphate dehydrogenase in the pentose phosphate pathway is localized in vanadocytes of the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.15. 441-446 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama,T.: "6-phosphogluconate dehydrogenase is a 45 kDa-antigen recognized by a monoclonal antibody S4D5 specific to vanadocytes in the vanadium-rich ascidian,Ascidia sydneiensis samea." J.Biochem.124. 377-382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 道端 齊: "ホヤのバナジウム濃縮に携わる特異な細胞、バナドサイトの謎を探る." 生物物理. 37. 315-320 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama,T.: "Highly selective accumulation of vanadium by ascidians from sea water -An approach from concerted study between biological and chemical viewpoint." Proc.I.T.I.T.Int.Symp.New Technol.Marine-Sphere. 75-78 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanamori,K.: "Reduction of vanadium(IV) to vanadium(III) by cysteine methylester in water in the presence of amino polycarboxylates." Chem.Letters. 423-424 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wuchiyama,J.: "Preparation and localization of a monoclonal antibody against a vanadium-associated protein extracted from the blood cells of the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 409-414 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nose,Y.: "Specific increase in the number of vaandium-accumulating blood cells by some ionophores and inhibitors of proton-ATPases in the ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 199-204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanda,T.: "Identification of a vanadium-associated protein from the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uyama,T.: "Finding of the same antigen in the polychaeta,Pseudopotamilla occelata,as those in the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 43-47 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michibata, H.: "Vanadocyte is a cell holding the key to resolve the highly selective accumulation and reduction of vanadium in ascidians." Microscop.Res.Technol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michibata, H.: "The accumulation mechanism of vanadium by ascidians-An interdisciplinary study between biology and chemistry on extraordinary high levels and reduced form of vanadium in vanadocytes." Am.Chem.Soc.Symp.Ser.711. 248-258 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michibata, H.: "Selective accumulation of vanadium by ascidians from seawater." Adv.Environm. Sci.& Technol. edit.Nriagu, J.John Wiley & Sons, Inc.217-249 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueki, T.: "Isolation of cDNAs encoding subunits A and B of the vacuolar-type ATPase from the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.15. 823-829 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T.: "A 100-kDa antigen recognized by a newly prepared monoclonal antibody specific to the vanadocytes of the vanadium-rich ascidian, Ascidia sydneiensis samea, is glycogen phosphorylase." Zool.Sci.15. 815-821 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T.: "Glucose-6-phosphate dehydrogenase in the pentose phosphate pathway is localized in vanadocytes of the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.15. 441-446 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T.: "6-phosphogluconate dehydrogenase is a 45 kDa-antigen recognized by a monoclonal antibody S4D5 specific to vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea." J.Biochem.124. 377-382 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michibata, H.: "Exploring the mystery of vanadocytes, cells specialized for the vanadium accumulation in ascidians" Seibutsubutsuri. 37. 315-320 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T.: "Highly selective accumulation of vanadium by ascidians from sea water-An approach from concerted study between biological and chemical viewpoint." Proc.I.T.I.T.Int.Symp.New Technol. Marine-Sphere. 75-78 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanamori, K.: "Reduction of vanadium (IV) to vanadium (III) by cysteine methyl ester in water in the presence of amino polycarboxylates." Chem.Letters. 1997. 423-424 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wuchiyama, J.: "Preparation and localization of a monoclonal antibody against a vanadium-associated protein extracted from the blood cells of the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.14. 409-414 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nose, Y.: "Specific increase in the number of vanadium-accumulating blood cells by some ionophores and inhibitors of proton-ATPases in the ascidian, Ascidia sydneiensis samea." Zool.Sci.14. 199-204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanda, T.: "Identification of a vanadium-associated protein from the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uyama, T.: "Finding of the same antigen in the polychaeta, Pseudopotamilla occelata, as those in the vanadium-rich ascidian, Ascidia sydneiensis samea." Zool.Sci.14. 43-47 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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