| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Fuminori Showa University, School of Medicine, Associate Professor, 医学部, 助教授 (30102346)
HASEGAWA Masami The Institute of Statistical Mathematics, Professor, 予測制御研究系, 教授 (60011657)
HASHIMOTO Tetsuo The Institute of Statistical Mathematics, Associate Professor (50208451)
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| Research Abstract |
In order to elucidate early evolution of eukaryotes, we have been studying phylogenetic relationship among protists using multiple molecular sequence data. In this project, we established the methods to get much DNAS needed for gene analyses of the protists, including Giardia lamblia (diplomonads), Trichomonas vaginalis (parabasalids), Entamoeba histolytica (entamoebidae), Trypanosoma cruzi (euglenozoa), Blastocystis hominis (stramenopiles), Plasmodium falciparum, Toxoplasma gondii, and Cryptosporidium muris (alveolata), Glugea plecoglossi, Encephalitozoon hellem, and E. cuniculi (microsporidia), by collaborating with the researchers in various institutions. On the basis of the materials of these protists, we sequenced the genes encoding translation elongation factors, 1 alpha and 2, isoleucyl- and valyl-tRNA synthetases, 70kDa heat shock protein, and other several molecules. Phylogenetic analyses including these original data clearly suggested 1) that all of the four amitochondriate protists lineages, diplomonads, parabasalids, entamoebidae, and microsporidia had lost their mitochondria secondarily during their evolution, 2) that Blastocystis hominis is involved in stramenopiles and the closest relative of stramenopiles is alveolata and 3)that microsporidia are close to fungi.
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