1998 Fiscal Year Final Research Report Summary
Molecular Cytogenetic Study of the Tarsier
| Project/Area Number |
09440282
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
人類学(含生理人類学)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIRAI Momoki Graduate School of Science, The University of Tokyo, Professor, 大学院・理学系研究科, 教授 (60156635)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Shoji Graduate School of Science, The University of Tokyo, Research Associate, 大学院・理学系研究科, 助手 (40282727)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Tarsier / chromosome / phylogenic relationship / replication banding / repeated sequence / synteny |
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| Research Abstract |
The evolution and phylogeny of the Tarsier (Genus Tarsius) have long been controvertial. In the present study, we attempted to perform molecular cytogenetic studies to explore the phylogenetic position of this genus. We cultured blood cell lymphocytes and kidney cells to prepare chromosome preparations. By addition of BrdU, metaphase chromosomes with replication bands were obtained. (1) We confirmed that the diploid chromosome number of T.Bancanus is 80. (2) Human X chromosome-specific painting probe hybridized with the whole tarsier X chromosome, suggesting its conservatism. The Y chromosome contains a species- specific repetitive sequences on its long arm. These repetitive sequences are not homologous with human DYZ1 on the Y choromosome. Human SRY probe hybridized to the telomeric portion of the short arm of the Y chromosome of this species. (3) Some human chromosome-specific painting probes revealed human-tarsier syntenic relationships. (4) We established tarsier kidney cell lines. (5) Tarsier genomic DNA library was established. (6) For comparative chromosome mapping, we collected human and mouse cDNA clones. First, these clones were precisely mapped to human chromosomes. Then the clones were licalized on tarsier chromosomes by fluorescence in situ hybridization. The data will be useful for determining the syntenic relationship between tarsier and human chromosomes.
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[Publications] Mizushima, S., Fujita, M., Ishida, T., Asuma, S., Kato, K., Hirai, M., Otsuka, M., Yamamoto, T., Inoue, J: "Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5)."Gene. 207. 135-141 (1998)
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[Publications] Kusuda, J., Hirai, M.m, Toyoda, A., Tanuma, R., Kitabayashi, A., Hashimoto, K.: "Cloning and chromosomal localization of a paralog and a mouse homolog of the human transaldolase gene."Gene. 209. 12-21 (1998)
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[Publications] Tsukahara, F., Urakawa, I., Hattori, M., Hirai, M., Ohba, K., Yoshioka, T., Sakaki, Y., Muraki, T.: "Molecular characterization of the mouse mtprd gene, a homologue of human TPRD : unique gene expression suggesting its critical role in the pathophysiology of Down syndrome."J. Biochem.. 123. 1055-1063 (1998)
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