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1998 Fiscal Year Final Research Report Summary

Fabrication and Characterization of Biomolecule-based Structure by Using SECM

Research Project

Project/Area Number 09450311
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 工業物理化学
Research InstitutionTohoku University

Principal Investigator

MATSUE Tomokazu  Tohoku Univ.Grad.Sch.Eng.Assoc.Prof., 大学院・工学研究科, 助教授 (70173797)

Project Period (FY) 1997 – 1998
KeywordsElectrochemical microscopy / SECM / Enzyme / Patterning / Microstructure / Cell / Respiration / Photosynthesis
Research Abstract

1. Scanning electrochemical microscopy (SECM) was used for localized electrogeneration of hydroxyl radical to create patterns with diaphorase on glass substrates with self-assembled mono-layers (SAMs) of different alkylsilane derivatives. The electrogenerated hydroxyl radical from the probe (microelectrode) of SECM diffused onto the SAM surfaces and degraded the SAMs and removed them from the glass surfaces. When the SAM was hydrophobic, diaphorase patterns were formed on the substrates by dipping the resulting substrate into a diaphorase solution. A SAM with functional terminal groups was also used to immobilize diaphorase via chemical linkage, giving a diaphorase pattern on the substrate.
2. SECM was used for imaging of cellular activity at the single cell level. SECM measurements of SW-480 based on the reduction current of oxygen gave images of respiration activity. The SECM measurements of living cells are particularly suitable to clarify the influence of chemical and physical stimu … More lation at a single cell level. When cyanide ion or nitrophenol was added to the culture medium, the SECM image of the cell became pale, indicating that these species lowers the respiration activity. The permeation of the cellular membrane to cyanide ion was evaluated by the quantitative analysis of the change in the cellular activity. Similarly, SECM was used for estimating the respiration and photosynthetic activity of a single plant cell.
3. SECM was used for estimation of permeation of several redox species through a cell membrane of a single algal protoplast. When the microelectrode is placed close to a membrane which influences the formation of the semi-spherical diffusion region, the redox current becomes lower than that observed in the bulk solution. Since the decrease in the redox current correlates with the permeability of the membrane to redox species, the quantitative analysis of the phenomena affords the permeation coefficient (Pm) for redox species to pass through the membrane. The Pm values for Fe(CN)_6^<-4>, Fe(CN)63-, Co(phen)32_3^<+2>, FMA and hydroquinone were <1x10-4, <1x10-4, 1x10^<-3>, 5x10^<-3> and 2x10^<-2> cm/sec, respectively. Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] H.Shiku: "Dual Immunoassay of Human Chorionic Gonadotropin and Human Placental Lactogen at a Microfabricated Substrate by Scanning Electrochemical Microscopy" J.Electroanal.Chem.438. 187-190 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末永智一: "走査型電気化学顕(SECM)による局所センシング" 化学センサ. 13. 8-13 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Shiku: "Microfabrication of Alkylsilanized Glass Substrate by Electrogenerated Hydroxyl Radical Using Scanning electrochemical Microscopy" Langmuir. 13. 7239-7244 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末永智一: "生体機能材料の局所センシング" 信学技報. 97. 17-21 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yasukawa: "Permeation of Redox Species Through a Cell Membrane of a Single, Living Algal Protoplast Studied by Microamperometry" Biochim.Biophys.Acta. 1369. 152-158 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yasukawa: "Microamperometric Determination of Photosynthetic Oxygen Generation from a Single Protoplast" Denki Kagaku. 66. 660-661 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yasukawa: "Imageing of Cellular Activity of Single Cells by Scanning Electrochemical Microscopy" Chem.Lett.767-768 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yasukawa: "Microamperometric Measurements of Photosynthetic Activity in a Single Algal Protoplast" Biophys.J.76. 1129-1135 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Shiku, Y.Hara, T.Matsue, I.Uchida, T.Yamauchi: "Dual Immunoassay of Human Chorionic Gonadotropin and Human Placental Lactogen at a Microfabricated Substrate by Scanning Electrochemical Microscopy" J.Electroanal.Chem.438. 187-190 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Localized Sensing with Scanning Electrochemi-cal Microscopy (SECM)" Chemical Sensors. 13. 8-13 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Shiku, I.Uchida, T.Matsue: "Microfabrication of Alkylsi-lanized Glass Substrate by Electrogenerated Hydroxyl Radical Using Scanning electrochemical Microscopy" Langmuir. 13. 7239-7244 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Matsue: "Microsensing of Biofunctional Materials" Technical Report of IEICE. OME97-80. 17-21 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yasukawa, I.Uchida, T.Matsue: "Permeation of Redox Species Through a Cell Membrane of a Single, Living Algal Protoplast Studied by Microamperometry" Biochim.Biophys.Acta.1369. 152-158 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yasukawa, I.Uchida, T.Matsue: "Microamperometric Determination of Photosynthetic Oxygen Generation from a Single Protoplast" Denki Kagaku. 66. 660-661 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yasukawa, Y.Kondo, I.Uchida, T.Matsue: "Imaging of Cellular Activity of Single Cells by Scanning Electrochemical Microscopy" Chem.Lett.767-768 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yasukawa, I.Uchida, T.Matsue: "Microamperometric Measurements of Photosynthetic Activity in a Single Algal Protoplast" Biophys.J.76. 1129-1135 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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