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1999 Fiscal Year Final Research Report Summary

Physiological and biochemical studies on leaf development and senescence : Degradation of Rubisco mediated by reactive oxygen species

Research Project

Project/Area Number 09460036
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MAE Tadahiko  Tohoku University, 大学院・農学研究科, 教授 (60134029)

Co-Investigator(Kenkyū-buntansha) ISHIDA Hiroyuki  Tohoku University, 大学院・農学研究科, 助手 (60312625)
MAKINO Amane  Tohoku University, 大学院・農学研究科, 助教授 (70181617)
Project Period (FY) 1997 – 1999
KeywordsRubisco / reactive oxygen species / light stress / oxidative stress / degradation / photosynthesis
Research Abstract

Rubilose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39, Rubiscso) is a bifunctional enzyme which catalyzes two competing reactions, photosynthetic COィイD22ィエD2 assimilation and photorespiratory carbon oxidation in the stroma of the chloroplasts, and is the most abundant protein in leaves. During leaf senescence or under environmental stress conditions, Rubisco is one of the early proteins that are broken down in leaves. However, only limited information is available on the triggering mechanisms that cause Rubisco degradation in plants. Here, we found that the large subunit of Rubisco is directly, fragmented into the 37-kDa and 16-kDa fragments by reactive oxygen species generated in the illuminated lysates of chloroplasts or intact chloroplasts. Analysis of the terminal amino acid residues of both fragments indicated that the fragmentation of the LSU occurs at Gly-329. Purified Rubisco, exposed to a hydroxyl radical generating system, was also cleaved at the same site. Reactive oxygen species generated at its catalytic site by a Fenton-type reaction may trigger the site specific degradation of the LSU in the light.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ishida H.: "The large subumit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into the 37-kD and 16-kD polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat"Plant Cell Physiol.. 38・4. 471-479 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida H.: "Light-dependent degradation of the large subumit of ribulose-1,5-bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves"Planta. 204・3. 305-309 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida H.: "Fragmentation of the large subumit of ribulose-1,5-bisphosphate carboxylase by active oxygen species occurs near Gly-329"J.Biol.Chem.. 274・8. 5223-5226 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 前忠彦: "Rubiscoは光合成に対して過剰存在するか?"化学と生物. 37・2. 113-114 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石田宏幸: "光ストレスとRubiscoの分解"化学と生物. 38・1. 8-9 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino A.: "Photosynthesis and plant growth at elevated levels of CO_2"Plant Cell Physiol.. 40・10. 999-1006 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 前忠彦: "生物学データ集"朝倉書店 金井龍二他編(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mae T.: "Leaf senescence and nitrogen metabolism. In"Programmed Cell Death and Related Processes""Ed.by Nooden, L., Academic, Press New York(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishida, H. ; Nishimori, Y. ; Sugisawa, M. ; Makino, A. ; Mae, T: "The large subunit of ribulose-1,5-bisphosphatecarboxylase/oxygenase is fragmented into the 37-kD and 16-kD polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat"Plant cell Physiol.. 38. 471-479 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida H., Shimizu, S., Makino A. and Mae, T: "Light-dependent degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves"Planta. 204. 305-309 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida, H., Makino, A., and Mae, T.: "Fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by active oxygen species occurs near Gly-329"J. Biol. Chem.. 274. 3222-5226 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mae. T: "Is Rubisco in excess?"Kagaku to Seibutu. 37. 113-114 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida, H., Makino, A., and Mae, T.: "Light stress and Rubisco degradation : Fragmentation of the large subunit of Rubisco mediated by reactive oxygen species"Kagaku to Seibutu. 38. 8-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mae, T.: "Physiological nitrogen efficiency in rice : Nitrogen utilization, photosynthesis, and yield potential. In "Proceedings of the XIII International Plant Nutrition Collocuium : Plant Nutrition for Sustainable Food Production and Environment" eds., T. Ando et al."Kluwer Academic Publishers, Dordrecht, The Netherlands.. 51-60 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makino, A., and Mae, T.: "Nitrogen partitioning to ribulose-1,5-bisphosphate and chlorophyll in the leaves of rice grown under different irradiances, temperatures, and partial pressures of COィイD22ィエD2. In "Proceedings of the 5ィイD1thィエD1 International Symposium on Inorganic Nitrogen Assimilation". Eds. Martins-Loucao and Cabrita M.T."Trialgrafica, Artes Graficas, Lda., Lisbon Portogue.. 79 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida, H., Makino, A., and Mae, T.: "Detection of the cleavage site of the large subunit of Rubisco by active oxygen species and its inhibition by CABP in the lysates of wheat chloroplasts. In "Proceedings of the XIth International Congress on Photosynthesis : Photosynthesis : Mechanisms and Effects" ed. Garab, G."Kluwer Academic Publishers, Dordrecht, The Netherlands.. 2245-2248 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishida, H., Shimizu, S., Makino, A., and Mae, T.: "Light-dependent fragmentation of ribulose-1,5-bisphospate carboxylase/oxygenase in chloroplasts isolated from wheat leaves. In "Proceedings of the XIth International Congress on Photosynthesis : Photosynthesis : Mechanisms and Effects" ed. Garab, G."Kluwer Academic Publishers, Dordrecht, The Netherlands.. 2249-2252 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mae, T.: "Leaf senescence and nitrogen metabolism. In "Programmed Cell Death ad Related Processes""Ed. by Nooden, L., Academic, Press New York (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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