Physiological and molecular biological study on the mechanisms for VFA-induced inhibition of GH release from somatotrophs

1998 Fiscal Year Final Research Report Summary

• Project/Area Number: 09460122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Tohoku University
• Principal Investigator: KATOH Kazuo AGRICULTURE TOHOKU UNIVERSITY,ASSOC.PROF., 農学部, 助教授 (60091831)
• Co-Investigator(Kenkyū-buntansha): HAGINO Akihiko AGRICULTURE TOHOKU UNIVERSITY,RES.ASSOC., 農学部, 助手 (80156249)
• Project Period (FY): 1997 – 1998
• Keywords: VFA / GH (GROWTH HORMONE) / GH SECRETING CELLS / SOMATOTROPHS

Research Abstract

(1) Effects of VFA on the basal and GHRH-induced GH release
When the cells incubated with butyrate or propionate for 72 hrs were stimulated with GHRH, GH release was significantly suppressed compared with the cells cultured in a medium without a fatty acid.
(2) Effects of VFA on membrane Ca current
When membrane potential was depolarized in a step manner, inward current was increased via the cell membrane. This current, which was completely abolished by nifedipine, was suppressed in a medium containing butyrate.
(3) Effects of VFA on cellular Ca ion concentrations
Butyrate itself slightly increased, but it rater suppressed GHRH-induced increase in cellular Ca concentration.
(4) Effects of VFA on GHmRNA content
Butyrate suppressed GHmRNA content.

Research Products

• [Publications] Ohata et al.: "Growth hormone release induced by an amino acid mixture from primary cultured anterior pituitary cells of goats." Domestic Animal Endocrinology. 14(2). 99-107 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 加藤和雄・C.Chen: "成長ホルモン放出を刺激する人工ペプチドに関する研究動向" 畜産の研究. 51(4). 501-505 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Katoh. & Ishiwata: "Changes in intracellular calcium concentration and growth hormone release induced by nutrients in primary cultured anterior pituitary cells of goats." Animal Science and Technology. 69. 994-1003 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 加藤和雄・石渡広子: "第3回家畜内分泌国際学会(The somatotropic axis)参加報告" 畜産の研究. 印刷中.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsunaga et al.: "Effects of ruminal infusion of volatile fatty acids on plasma concentration of growth hormone and insulin in sheep." Domestic Animal Endocrinology. in press.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Katoh et al.: "Suppressing effects of short-chain fatty acids on GHRH-induced GH release in isolated anterior pituitary cells of goats." Domestic Animal Endocrinology. in press.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohata, Y., Maruyama, Y., Katoh, K.and Sasaki, Y.: "Growth hormone release induced by an amino acid mixture from primary cultured anterior pituitary cells of goats." Domestic Animal Endocrinology. 14 (2). 99-107 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 51 (4). 501-505 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Katoh, K.and Ishiwata, H.: "Changes in intracellular calcium concentration and growth hormone release induced by nutrients in primary cultured anterior pituitary cells of goats." Animal Science and Technology. 69. 994-1003 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsunaga, N., Arakawa, N.T., Goka, T., Nam, K.-T., Ohneda, A., Sasaki, Y.and Katoh, K.: "Effects of ruminal infusion of volatile fatty acids on plasma concentration of growth hormone and insulin in sheep" Domestic Animal Endocrinology. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Katoh, K., Ohata, Y.and Ishiwata, H.: "Suppressing effects of short-chain fatty acids on GHRH-induced GH release in isolated anterior pituitary cells of goats." Domestic Animal Endocrinology. (in press).
  • Description: 「研究成果報告書概要(欧文)」より