2000 Fiscal Year Final Research Report Summary
STUDY ON THE CONSERVATION OF AVIAN GNETIC RESOURCES USING PRIMORDIAL GERM CELLS (PGC)
| Project/Area Number |
09460123
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | UNIVERSITY OF TSUKUBA |
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Principal Investigator |
TAJIMA Atsushi UNIVERSITY OF TSUKURA, INSTITUTE OF AGRICULTURE AND FORESTRY, ASSOCIATE PROFESSOR, 農林学系, 助教授 (40207030)
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| Project Period (FY) |
1997 – 2000
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| Keywords | AVIAN / GENETIC RESOURCES / PRIMRODIAL GERM CELLS / PGC |
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| Research Abstract |
RESRCH ON THE CONSERVATION OF AVIAN WAS CONDUCTED.
AT PRESENT, AVIAN GENETIC RESOURCES ARE CONSERVED AS FLOCK SINCE IT IS NOT POSSILBLE TO CONSERVE FERTILIZED EMBRYOS FOR MORE THAN 2-3 WEEKS WITHOUT IMPAIRING HATCHABILITY.NEW STRATEGY TO CONSERVE AVIAN GENETIC RESOURCES NEEDS TO BE DEVELOPED.TO ACCHIEVE THE GOAL, GERM-LLINE CHICMERAS WERE PRODUCED BY TRANSFERRING PRIMORDIAL GERM CELLS (PGC).
REDULTS OBTAINED IN THE PRESENT RESEARCH PROJECT WAS AS FOLLOWS : 1) NUMBER OF THE CIRCULATING PGC WAS HIGHEST AT STAGE 14 AND DECREASED THEREAFTER.2) TOTAL NUMBER OF CIRCULATINF PGC WAS ESTIMATED TO BE 807 AND 857 AT STAGE 14 AND 15, RESPECTIVELY.3) THE REASON FOR THE CONSIDERABLE FULCTUATION OF THE PCG CONCENTRATION IN EARCLY CHICK EMBRYO WAS PRIMARIRILY ATTRIBUTED TO THE FEMALE THAT PRODUCE EGG.4) GERM-LINE CHIMERAS WERE PRIDUCED BY TRANSFEERING CRYOPRESERVED GONADAL PGC (GPGC) COLLECTED FROM 5-DAY-OLS CHICK EMBRYO.5) NEW METHOD OF COLLECTING COCULATING PGC USING FILTRATION METHOD WAS DEVELOPED.
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