• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

Mammalian Reproductive Pheromone : Isolation and Study on Central Action.

Research Project

Project/Area Number 09460124
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionThe University of Tokyo

Principal Investigator

MORI Yuji  Graduate School of Agricultural and Life Science, The Univ. Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (40157871)

Co-Investigator(Kenkyū-buntansha) ICHIKAWA Masumi  Tokyo Metropolitan Inst. for Neurosci., Chief of Research Staff, 主任研究員 (20124414)
KIKUSUI Takehumi  Graduate School of Agricultural and Life Science, The Univ. Tokyo, Assistant Professor, 大学院・農学生命科学研究科, 助手 (90302596)
TAKEUCHI Yukari  Graduate School of Agricultural and Life Science, The Univ. Tokyo, Associate Professor, 大学院・農学生命科学研究科, 助教授 (10240730)
Project Period (FY) 1997 – 1999
KeywordsPheromone / GnRH / Multipleunit activity / Reproductive endocrinology / Male effect / Accessory olfactory system / Hypothalamus / Shiba goat
Research Abstract

The purpose of the present study was to characterize the mechanisms of production, perception and brain stimulation of mammalian reproductive pheromones.
(1) Pheromone production : castrated male Shiba goats were subcutaneously implanted with testosterone (T), dihydrotestosterone (DHT) or eatradiol, and the effects on the production of male pheromone and on the development of sebaceous glands were examined. Androgen-induced sebaceous gland development was limited to the head region, whereas pheromone production was observed not only in the head but also in the rump region following DHT treatment The results suggest that pheromone production is not necessarily accompanied by sebaceous gland development implicating the existence of independent regulatory mechanisms for these two responses.
(2) Pheromone perception : polyclonal antibody was produced against rat-pheromone receptor, and immunohistochemical analysis with it revealed the localization on the microvilli of vomeronasal nerves. Based on the sequence of genes encoding rat putative pheromone receptors the homologue genes were examined in the goat which identified the expression of type 2 homologue genes but not the type 1 family. Moreover, the analysis of G protein alpha subunits showed that the distinct region specific localization of Gi2 and Go in rodent species was not the case for other species such as goats, horses, dogs and marmosets.
(3) Brain stimulation: Recording electrodes were placed in the medial basal hypothalamus of ovariectomized goats, and the specific MUA volleys were recorded to monitor the electrophysiological manifestation of the GnRH pulse generator activity. The exposure to the pheromone resulted in the immediate acceleration of the GnRH pulse generator activity and the duration of the effect corresponded well with the time of exposure. The central action of primer pheromone was thus demonstrated neurophysiologically on the real-time basis.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Matsuoka M., Kaba H., Mori Y., Ichikawa M.: "Synaptic plasticity in olfactory momory formation in female mice."Neuroreport. 8. 2501-2504 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuoka M., Kaba H., Mori Y., Ichikawa M.: "Morphological changes of synapses induced by urinary stimulation in the hamster accessory olfactory bulb."Synapse. 28. 160-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ichimaru T., Takeuchi Y., Mori Y.: "Stimulation of the GnRH pulse gernerator activity by continuous exposure to the male pheromones in the female goat."J. Reprod. Develop.. 45. 243-248 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishihara M., Takeuchi Y., Tanaka T., Mori Y.: "Eletrophysiological correlates of pulsatile and surge gonadotropin secretion."J. Reprod.. 4. 110-116 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takigami S., Osada T., Mori Y., Ichikawa M. et al.: "The expressed localization of rat putative pheromone receptors."Neurosci. Lett.. 272. 115-118 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwata E., Wakabayashi Y., Takeuchi Y., Mori Y. et al.: "Primer pheromine is producedf testosterone dependently in the sebaceous gland of male goat."Biol. Rerod.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori Y., Tanaka T., Hamada T., Takeuchi Y.et al.: "Neural control of reproduction."Scientific Societies Press/Karger. 254 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森裕司,武内ゆかり他: "脳と生殖、-GnRH神経系の進化と適応-"学会出版センター. 233 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuoka M., Kaba H., Mori Y., Ichikawa M.: "Synaptic plasticity in olfactory momory formation in female mice."Neuroreport. 8. 2501-2504 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi Y., Nagabukuro H., Kizumi O., Mori Y.: "Lipopolysaccharide-induced suppression of the hypothalamic gonadotropin-releasing hormone pulse generator in ovariectomized goats."J. Vet. Med. Sci.. 59. 93-96 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka T., Ohgata N., Kamomae H., Takeuchi Y., Mori Y., Karsch FJ.: "Hypophyseal portal blood collection for analysis of GnRH neurosecretion in Shiba goats"J. Reprod. Develop. 43. 101-106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori Y., Tanaka T., Hamada T., Takeuchi Y.: "External and internal signals regulating GnRH secretion in ruminant species."Neural control of reproduction. 103-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuoka M., Mori Y., Ichikawa M.: "Morphological changes of synapses induced by urinary stimulation in the hamster accessory olfactory bulb."Synapse. 28. 160-166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichimaru T., Takeuchi Y., Mori Y.: "Stimulation of the GnRH pulse generator activity by continuous exposure to the male pheromones in the female goat."J. Reprod. Develop.. 45. 243-248 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura H., Kikusui T., Takeuchi Y., Mori Y.: "Estradiol-induced inhibition of body weight gain and reduction of food intake are not associated with diurnal rhythmicity."J. Reprod. Develop.. 45. 259-264 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishihara M., Takeuchi Y., Tanaka T., Mori Y.: "Electrophysiological correlates of pulsatile and surge gonadotropin secretion."Reviews of Reproduction. 4. 110-116 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takigami S., Osada T., Yoshida-Matsuoka J., Matsuoka M., Mori Y., Ichikawa M.: "The expressed localization of rat putative pheromone receptors."Neurosci. Lett.. 272. 115-118 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida-Matsuoka J., Osada T., Mori Y. Ichikawa M.: "A developmental study using three antibodies (VOBM1, VOBM2, and VOM2) : immunocytochemical and electron microscopic analysis of the luminal surface of the rat vomeronasal sensory epithelium."Anat. Embryol.. 199. 215-224 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata E., Wakabayashi Y., Kakuma Y., Kikusui T., Takeuchi Y., Mori Y.: "Primer pheromone is produced testosterone-dependently in the sebaceous gland of male goat."Biol. Reprod.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takigami S., Mori Y., Ichikawa M.: "Projection pattern of vomeronasal neurons to the accessory olfactory bulb in goats."Chem Senses. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi