1999 Fiscal Year Final Research Report Summary
Studies on new diagnosis and classification of diabetes in dogs and cats
| Project/Area Number |
09460148
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Nippon Veterinary and Animal Science University |
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Principal Investigator |
ARAI Toshiro Nippon Vet Anim Sci Univ, Dept Vet Sci, Assoc Prof, 獣医畜産学部, 助教授 (70184257)
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| Co-Investigator(Kenkyū-buntansha) |
SAKO Toshinori Nippon Vet Anim Sci Univ, Dept Vet Sci, Assoc Prof, 獣医畜産学部, 助教授 (70153971)
TSUCHIDA Shuichi Nippon Vet Anim Sci Univ, Dept Vet Sci, Lecturer, 獣医畜産学部, 講師 (20217326)
SAITO Tohru r. Nippon Vet Anim Sci Univ, Dept Vet Sci, Assoc Prof, 獣医畜産学部, 助教授 (70211258)
OHGAWARA Hisako Tokyo Womens Med Coll, Med Res Inst, Assoc Prof, 医学部, 助教授 (10075468)
OHISHI Iwao Nippon Vet Anim Sci Univ, Dept Vet Sci, Professor, 獣医畜産学部, 教授 (50081592)
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| Project Period (FY) |
1997 – 1999
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| Keywords | dogs and cats / diabetes / insulin / glucokinase / PCR / streptozotocin / artificial pancreas / gene diagnosis |
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| Research Abstract |
1. New diagnosis for diabetes in dogs : Plasma glucose concentrations have been used as diagnostic indicator to assess metablic conditions in dogs. White blood cell (WBC) dlucokinase (GLK) activities and mRNA were measured in streptozotocin-induced type 1 diabetic dogs. Plasma glucose concentrations decreased remarkably but mRNA qunatitation by RT-PCR was not recovered after insulin injections in the diabetic dogs. WBC GLK activities and mRNA exprerssion were considered to be a good diagnostic indicator to assess the diabetic condition in dogs.
2. Transplantation of artificial pancreas : A diffusion chamber as bioartificial endocrine pancreas (Bio-AEP) constructed by placing pancreatic endocrine cells, which were shielded by silicone, was implanted to streptozotocin (STZ)-induced diabetic rats. In the STZ diabetic rats with Bio-AEP, a return to normoglycemia was observed up to 30 weeks after implantation, without the use of any immunosuppressant. Xenoimplantation using the Bio-AEP might improve endocrine insulin release and retard the progress of diabetes.
3. Development of kits to measure plasma insulin in dogs and cats : New ELISA kits with specific antibodies against dog and cat insulin were developed. These ELISA system showed high reproducibility for measurement of plasma insulin concentrations in dogs and cats.
4. Herbal treatment for diabetes in dogs : A kind of Chinese herbal medicine, Echvaria glauca, was used for treatment of STZ-induced diabetic dogs. The oral treatment with herb gave a remarkable improvement of the diabetic condition by causing recovery of hepatic function.
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[Publications] Hirotani, S., Eda, R., Kawabata, T., Fuchinoue, S., Teraoka, S., Agisi, T. and Ohgawara, H.: "Bioartificial endocrine pancreas (Bio-AEP) for treatment of diabetes : Effect of implantation of Bio-AEP on the pancress"Cell Transplantation. 8. 399-404 (1999)
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[Publications] Arai, Y., Hashimoto, K., Mizutani, H., Kawabata, T., Sako, T. and Washizu, T.: "A comparison of the plasma fructose concentrations in dogs and cats and changes in the fructose concentrations in dogs following intyravenous administration of fructose"Veterinary Research Communications. 23. 203-209 (1999)
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[Publications] Saito, R.T., Saito, M., Arai, T., Aoki-Komori, S., Taniguchi, K. and Takahashi, W.K.: "p-Chloroamphetamine (PCA) suppresses ingestive behavior in male rats."Experimental Animals. 48. 263-267 (1999)
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[Publications] Arai, T., Takahashi, S., Tanaka, A., Sasaki, N., Kawabata, T. and Sako, T.: "Changes in activities of blood cell enzymes in diabetic dogs treated with herb (Nikasyou)"Bulletin of Nippon Veterinary and Animal Science University. 48. 15-20 (1999)
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