2000 Fiscal Year Final Research Report Summary
Studies for mechanisms of experimental oral immune-tolerance
| Project/Area Number |
09460149
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Azabu University |
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Principal Investigator |
YAMADA Takatsugu Azabu University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (20126460)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Motoo Azabu University, School of Environmental Health, Professor, 環境保健学部, 教授 (50139531)
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| Project Period (FY) |
1997 – 2000
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| Keywords | dog / co-stimulatory signal / CD28 / B-7 / CD86 / genomic DNA / polyclonal antibodies / cDNA |
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| Research Abstract |
(1) Studies on the effects on canine systemic and local immunity by hemocyanin (KLH) oral administration : CD5 or CD8 positive cells were decreased at 44 days after KLH administration. In KLH administrated group, the mitogen reactions to Con A or KLH were also decreased.
(2) The effects on oral immuno-tolerance by Japanese cedar pollen oral administration : CD4 positive cells in BALF cells were increased and the reactions to Cry-j in BALF cells were suppressed in cedar pollen treated group. The reactions to Cry-j in peripheral lymphocytes were also suppressed in treated group.
(3) Three types of peptide antibodies for canine CD28 were established by immunizing synthesized peptide to rabbits and prepared IgG or Fab fraction of these antibodies. These antibodies recognized CD28 molecules in lysate of lymphocytes by western blotting. However, they could not recognize CD28 molecules on lymphocyte surface.
(4) Canine CD86 cDNA were cloned by Yang. In this experiments, canine CD86 genomic DNA were cloned. The sequences of genomic DNA for canine CD86 were completely same with CD86-mRNA complete code between base number 961-1854. Canine CD86 genomic DNA did not contain intron in this region.
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[Publications] Waritani, T., Okuno, Y., Ashida, Y., Tsuchiya, R., Kobayashi, K.and Yamada, T.: "Development and characterization of monoclonal antibodies against canine trypsin."Vet.Immunol.Immunopathol.. (In press). (2001)
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[Publications] Ochi, M., Yamada, T., Nishizono, K., Ashida, Y., Furuichi, M., Tsuchiya, R., Kitao, S.and Kobayashi, K.: "Measurement of feline lymphocyte mitogen reaction by propidium iodide (PI) fluorometry (in Japanese)"Jpn.J.Vet.Clin.Pathol.. 5. 31-34 (1999)
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[Publications] Yamada, T., Kawano, E., Nishizono, K., Ashida, Y., Shida, T.and Kobayashi, K.: "Evaluation of canine red-blood-cell-bound IgG, IgM, and C3 by mean of a combination of ELISA and the ether-extraction method (in Japanese)"Jpn.J.Vet.Med.Assoc.. 52. 651-857 (1999)
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[Publications] Yamada, T., Fujita, E., Kitao, S., Ashida, Y., Nishizono, K., Tsuchiya, R.and Kobayashi, K.: "Serum alpha-fetoprotein values in dog with various hepatic diseases."J.Vet.Med.Sci.. 61. 657-659 (1999)
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[Publications] Tsuchiya, R., Kyotani, K., Scott, M., Nishizono, K., Ashida, Y., Mochizuki, T., Kitao, S., Yamada, T., and Kobayashi, K.: "Role of platelet activating factor in development of thrombocytopenia and neutropenia in dogs with endotoxemia."Amr.J.Vet.Res.. 60. 216-221 (1999)
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[Publications] Matsuda, M., Kagawa, S., Sakamoto, Y., Miyajima, M., Barton, M.and Moore, J.E.: "Detection of hetero genus genotype among Australian strains of Taylorella equigenitalis."Aust.Vet.J.. 78. 56-57 (2000)
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