2000 Fiscal Year Final Research Report Summary
Gene expression and control mechanisms of the plant mycoplasmas
| Project/Area Number |
09460155
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAMBA Shigetou Graduate School of Frontier Sciences, The University of Tokyo Professor, 大学院・新領域創成科学研究科, 教授 (50189221)
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| Project Period (FY) |
1997 – 2000
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| Keywords | plant mycoplasma / pathovar / Phytoplasma asteri / pathogen / microorganisms / insect vectors / bacteria / plant |
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| Research Abstract |
Plant mycoplasmas (phytoplasmas) are plant pathogenic bacteria inhabiting in the plant cell cytoplasm and transmitted by insect vectors. Among all known microorganisms, they are the smallest in cell size and in genome size. This study was performed to elucidate molecular mechanisms of host determination of plant mycoplasmas and to established a system for gene introduction into the bacteria. The results are expected to contribute to the control of this pathogen which causes serious damage to more than 600 plant species.
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[Publications] Nishigawa,H.,Miyata,S.,Oshima,K.,Sawayanagi,T.,Komoto,A.,Kuboyama,T.,Matsuda,I.,Tsuchizaki,T.and Namba,S: "In planta expression of a protein encoded by the extrachromosomal DNA of phytoplasma and related to geminivirus replication proteins."Microbiology. 147. 507-513 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Oshima,K.,Kakizawa,S.,Nishigawa,H.,Kuboyama,T.,Miyata,S.,Ugaki,M.and Namba,S.: "A Plasmid of phytoplasma encodes a unique replication protein having both plasmid-and virus-like domains : Clue to viral ancestry of result of virus/plasmid recombination?"Virology. (in press). (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Namba, S., Sawayanagi, T., Yamamoto, S., Lu, X., Kagami, T., Shiomi, T.and Tsuchizaki, T.: "Phylogenetic analysis of plant mycoplasmas occurring in the world."Japanese Journal of Mycoplasmology. 24. 100-102 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sawayanagi, T., Kagami, T., Lu, X., Shiomi, T., Tsuchizaki, T.and Namba, S.: "Phylogenetic analysis of phytoplasmas based on 16S/23S rRNA internal transcribed spacer regions."Japanese Journal of Mycoplasmology. 24. 103-105 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kagami, K., Shiomi, T., Tanaka, M., Fukuzawa, H., Sawayanagi, T., Sun, L., Lu, X., Tsuchizaki, T.and Namba, S.: "Pathogenicity of plant mycoplasma and extrachromosomal DNA."Japanese Journal of Mycoplasmology. 24. 106-108 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, Y., Sawayanagi, T., Kagami, T., Tominaga, A., Sun, L., Lu, X., Tanaka, M., Shiomi, T., Tsuchizaki, T.and Namba, S.: "Genome of plant mycoplasma."Japanese Journal of Mycoplasmology. 24. 109-110 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shiomi, T., Tanaka, M., Sawayanagi, T., Yamamoto, S., Tsuchizaki, T.and Namba, S.: "A symptomatic mutant of onion yellows phytoplasma derived from a greenhouse-.maintained isolate"Ann.Phytopath.Soc.Japan. 64. 501-505 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sawayanagi, T., Nishikawa, H., Horikoshi, N., Kanehira, T., Shinohara, M.and Namba, S.: "Phylogenetic analysis of Japanese Hydrangea phyllody phytoplasma using the 16S rRNA gene."Japanese Journal of Mycoplasmology. 25. 127-129 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kuboyama, T., Lu, X., Sawayanagi, T., Kanazawa, T., Nishigawa, H., Arai, S., Oshima, K., Matsuda, I., Tsuchizaki, T.and Namba, S.: "A rolling-circle replication plasmid, pOYW1, isolated from phytoplathogenic onion yellows phytoplasma."Japanese Journal of Mycoplasmology. 25. 124-126 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kuboyama, T., Huang, C.-C., Lu, X., Sawayanagi, T., Kanazawa, T., Kagami, T., Matsuda, I., Tsuchizaki, T.and Namba, S.: "A plasmid isolated from phytopathogenic Onion Yellows phytoplasma and its heterogeneity in thesymptomatic phytoplasma mutant."Molecular Plant Microbe Interaction. 11. 1031-1037 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kanazawa, T., Sawayanagi, T., Tanaka, M., Nishigawa, H., Kuboyama, T., Arai, S., Oshima, K., Matsuda, I., Tsuchizaki, T.: "Characterization of an isolated symptomatic onion yellows phytoplasma mutant."Japanese Journal of Mycoplasmology. 25. 121-123 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sawayanagi, Ttoshimi, Horikoshi, N., Kanehira, T., Shinohara, M., Lu, X., Kagami, T., Huang, C.-C.and Namba, S.: ""Candidatus phytoplasma japonicum, " a new phytoplasma taxon associated with Hydrangea phyllody."International Journal of Systematic Bacteriology. 49. 1275-1285 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uehara, Tamaki, Tanaka, M., Shiomi, T., Namba, S., Tsuchizaki, T.and Matsuda, I.: "Histopathological studies on two symptom types of phytoplasma associated with lettuce yellows."Ann.Phytopath.Soc.Japan. 65. 465-469 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sawayanagi, T., Horikoshi, N., Kanehira, T., Shinohara, M., Oshima, K.and Namba, S.: "'Candidatus Phytoplasma japonicum', a new phytoplasma taxon associated with Japanese Hydrangea phyllody."Japanese Journal of Mycoplasmology. 26. 30-33 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Oshima, K., Kagami, T., Kuboyama, T., Kanazawa, T., Nishigawa, H., Sawayanagi, T., Tanaka, M., Matsuda, I., Tsuchizaki, T.and Namba, S.: "Characterization of an extrachromosomal DNA (OYEW1) from onion yellows phytoplasma."Japanese Journal of Mycoplasmology. 26. 34-36 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Komoto, A., Oshima, K., Kanazawa, T., Sawayanagi, T., Arai, S., Matsuda, I., Tsuchizai, T., Kuboyama, T.and Namba, S.: "Characterization of thymidylate kinase gene from OY-Phytoplasma."Japanese Journal of Mycoplasmology. 26. 37-39 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nishigawa, H., Miyata, S., Oshima, K., Sawayanagi, T., Komoto, A., Kuboyama, T., Matsuda, I., Tsuchizaki, T.and Namba, S.: "In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins."Microbiology. 147. 507-513 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Oshima, K., Kakizawa, S., Nishigawa, H., Kuboyama, T., Miyata, S., Ugaki, M.and Namba, S.: "A Plasmid of phytoplasma encodes a unique replication protein having both plasmidand virus-like domains : Clue to viral ancestry or result of virus/plasmid recombination ?"Virology. (in press). (2001)
Description
「研究成果報告書概要(欧文)」より
