1998 Fiscal Year Final Research Report Summary
Circadian clock function as a neuron-glia complex
| Project/Area Number |
09470018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Waseda University |
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Principal Investigator |
SHIBATA Shigenobu Waseda University School of Human Sciences, Pharmacology, Professor, 人間科学部, 教授 (10162629)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Circadian / biological rhythm / suprachiasmatic nucleus / glia / GFAP / clock / serotonin / mutant animals |
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| Research Abstract |
In order to clarify the role of GFAP-expressing astrocytes in the circadian clock, we compared the activity rhythms of GFAP mutant mice to those of wild-type mice in various lighting conditions. GFAP mutant mice exhibited stable circadian rhythms both in light-dark cycles and constant darkness and showed normal entrainment to environmental light stimuli. However, under constant lighting conditions, the period of the activity rhythm in GFAP mutant mice was longer and more disrupted than in wild-type mice. HPLC analysis revealed that serotonergic activity in the suprachiasmatic nucleus, which is the center of the circadian clock, and the raphe nuclei was reduced m GFAP mutant mice. Furthermore, housing for 80 days under constant light decreased GFAP expression in the suprachiasmatic nucleus of C57BL/6J mice while increasing GFAP expression in the intergeniculate leaflet. These results demonstrate that the activities of GFAP expressed in astrocytes were changed in the suprachiasmatic nucleus and the intergeniculate nucleus under constant lighting conditions. Astrocytes in the suprachiasmatic nucleus may play important roles in the maintenance of circadian rhythms under constant lighting conditions via regulation of serotonergic activity.
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[Publications] Shishido Y,Furushiro M,Tanabe S,Taniguchi A,Hashimoto S,Yokokura T,Shibata S,Yamamoto T,Watanabe S: "Effect of ZTTA,a prolyl endopeptidase inhibitor, on memory impairment in a passive avoidance test of rats with basal forebrain lesions." Pharm Resl. 15. 1907-1910 (1998)
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[Publications] Akiyama, M., Moriya, T., Takahashi, S., Kouzu Y., Wakamatsu, H., and Shibata, S.: "Inhibition of light-or glutamate-indused mPerl expression represses the phase shifts into the mouse circadian locomotor and suprachiasmatic firing rhythms." J.Neuroscience. 19. 1115-1121 (1999)
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[Publications] Moriya, T., Ikeda, M., Yoshinobu, Y., Akiyama, M., Shibata, S.: "Potentiating action of MKC-242, a selective 5-HT1A receptor agonist, on the photic entrainment of the cicadian activity rhythm in hamsters." Br.J.Pharmacol. 125. 1281-1287 (1998)
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[Publications] Hamada, T., Sonoda, R., Watanabe, A., Ono, M., Watanabe, S., and Shibata, S.: "NMDA indused glutamate release from a rat suprachiasmatic nucleus in vitro." Neuroscience Letters. 256. 93-96 (1998)
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