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1999 Fiscal Year Final Research Report Summary

A new type of cytokine derived from lymphocytes affecting on nervous-endocrine system : Searching by fuzzy set cloning method

Research Project

Project/Area Number 09470028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

YANAGIHARA Nobuyuki  Dept. of Pharmacology, University of Occupational and Environmental Health, Professor, 医学部, 教授 (80140896)

Project Period (FY) 1997 – 1999
KeywordsCatecholamine efflux / Cytokine / Interactions among nervous-endocrine-immune system / Fuzzy set cloning / Adrenal medulla / Lymphocyte / Lymphocyte-derived factor / Bovine spleen cDNA library
Research Abstract

Cytokines were originally reported to be generated during the interaction of lymphocytes with specific antigens or non-specific mitogens and to function as communication signals among the immune systems. To investigate an interaction between lymphocytes and neurons or endocrine cells, and to seek for a new cytokine, the effects of lymphocytes and their conditioned medium on catecholamine efflux were examined in cultured bovine adrenal medullary cells. 1) Co-culture of adrenal medullary cells with lymphocytes for 3 days enhanced catecholamine efflux from the cells. 2) A conditioned medium prepared by incubation of lymphocytes for 3 days also stimulated catecholamine efflux in a time-and concentration-dependent manner. 3) Heat treatment of the conditioned medium at 60℃ and 100℃ reduced stimulatory effect to 59 and 20% of control. The stimulatory activity of the conditioned medium was almost observed in a high molecular fraction after dialysis (<8kDa cutoff) or gel filtration. 4) The cond … More itioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [ィイD13ィエD1H] norepinephrine uptake by the cells. These findings suggest that lymphocytes release a heat-sensitive and high molecular (>8kDa) factor (lymphocyte-derived factor) which stimulates catecholamine efflux from adrenal medullary cells.
Lymphocyte-derived factor(s)(LDFs) were partially purified by precipitation with 100% ammonium sulfate and followed by DEAE-cellulose column chromatography. Three peaks of active fraction were collected and pooled. Two antisera against the peak l and peak 2 were prepared and named as fuzzy antibody land antibody 2. Using the fuzzy antibody 1, 20 positive clones were isolated from bovine spleen cDNA library and partially sequenced. In these clones sequenced, the sequences of several clones (NY105, NY10, NY11) were new one when searched by the GENBANK or EMBL databases. The clones of NY16, NY18 and NY5 were found to be a new isoform of IgM, intron region for ornithine decarboxylase and one kind of serine protease, respectively. Less

  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Eiichi Tachikawa: "Interferon-α reduces catecholamine secretion from bovine adrenal chromaffin cells stimul・ated by acetycholine"Naunyn-Schmiedeber's Archieve of Pharmacology. 356. 699-705 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeki Yamamoto: "Parathyroid hormone-related peptide-(1-34)[PTHrP-(1-34)]induces vasopressin release from the rat supraoptic nucleus in vitro through a novel receptor"Endocrinology. 138. 2066-2072 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeki Yamamoto: "C-Type natriuretic peptide suppresses argine-vasopressin secretion from dissociated magnocellular neurons in newbarn rat supraoptic nucleus"Neuroscience Letters. 229. 97-100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyuki Yanagihara: "Stimulatory effect of lymphocyte-derived factor on catecholamine efflux from cultured bovine adrenal medullary cells"Neuroscience Letters. 243. 25-28 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yumiko Toyohira: "Down-regulation of the noradrenaline transporter by interferon-α in cultured bovine adrenal medullary cells"Journal of Neurochemistry. 70. 1441-1447 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhito Uezono: "Activation of inwardly recifying K^+ channels by GABA-B receptors expressed in Xenopus oocytes"Neuroreport. 9. 583-587 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yosuke Oishi: "Transmural compression-induced proliferation and DNA synthesis through activation of a tyrosine kinase pathway in rat astrocytome RCR-1 cells"Brain Research. 781. 159-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhito Uezono: "Adrenomedullin increases intracellular Ca^<2+> and inositol 1,4,5-trisphosphate in human oligodendroglial cell line KG-1C"Brain Research. 786. 230-234 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiji Yoshimura: "An active metabolite of carbamazepine, carbamazepine-10,11-epoxide, inhibits ion channel-mediated catecholamine secretion in clutured adrenal medullary cells"Psychopharmacology. 135. 368-373 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiko Tanaka: "Pituitary adenylate cyclase-activating polypeptide caurses Ca^<2+> release from ryanodine/caffeine stores though a novel pathway independent of both inositol trisphosphates and cyclic AMP in bovine adrenal medullary cells"Journal of Neurochemistry. 70. 1652-1661 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reji Yoshimura: "Carbamazepine-induced up-regulation of voltage-dependent Na^+ channels in bovine adrenal medullary cells in culture"The Journal of Pharmacology and Experimental Therapeutics. 287. 441-447 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koji Kajiwara: "Atherogenic lipoproteins inhibit catecholamine secretion in cultured bovine adrenal medullary cells"Naunyn-Schimiedeberg's Archivers of Pharmacology. 358. 308-314 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koji Hara: "Ketamine interacts with the noradrenaline transporter at a site partly overlapping the desipramine binding site"Naunyn-Schimiedeberg's Archives of Pharmacology. 358. 328-333 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柳原 延章: "副腎髄質クロマフィン細胞におけるCa^<2+>情報伝達機序"蛋白質核酸酵素. 43・12. 1777-1783 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Megumi Aso: "cDNA cloing of inducer reactive protein in adult T-cell lekemia cells by applying the fuzzy set cloning theory for sequencing defuzzification"Biomedical Soft Computing and Human Sciences. 5. 15-20 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi Shibuya: "Prostaglandin E_2 induces Ca^<2+> release from ryanodine/caffeine-sensitive stores in bovine adrenal medullary cells via EP_1-like receptors"Journal of Neurochemistry. 73. 2167-2174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koji Kajiwara: "Modification of low density lipoprotein potentiates its inhibitory effect on catecholamine secretion in cultured bovine adrenal medullary cells"Naunyn-Schimiedeberg's Archives of Pharmacology. 360. 99-102 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyuki Yanagihara: "Characterization and functional role of leptin receptor in bovine adrenal medullary cells"Biochemical Pharmacology. 59(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiji Yoshimura: "Inhibitory effects of clozapine and other antipsychotic drugs on noradrenaline transporter in cultured bovine adrenal medullary cells"Psychopharmacology. in press. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eiichiro Nakamura: "ATP activates DNA synthesis through a MAP kinase-dependent pathway by acting on P2X receptors in human osteoblast-like MG-63 cells"The American Journal of Physiology. in press. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyuiki Yanagihara: "Catecholamines : Bridging Basic Science with Clinical Medicine"Academic Press. 18-21/1084 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuyuki Yanagihara: "The Adrenal Chromaffin Cell"Hokkaido University Press. 243-247/394 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yumiko Toyohira: "Control Mechanisms of Stress and Emotion : Neuroendocrine-Based Studies"Elsevier Science. 263-264/359 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eiichi, Tashikawa: "Interferon-α reduces catecholamine secretion bovine adrenal chromaffin cells stimulated by acetylcholine"Naunyn-Schmiedeberg's Archieve of Pharmacology. 356. 699-705 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeki, Yamamoto: "Parathyroid hormone-related peptide-(1-34)[PTHr-(1-34)] induces vasopressin release from the rata supraoptic nucleus in vitro through a novel receptor"Endocrinology. 138. 2066-2072 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeki, Yamamoto: "C-Type natriuretic peptide suppresses argine-vasopressin secretion from dissociated magnocellular neurons newbarn rat supraoptic nucleus"Neuroscience Letters. 229. 97-100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyuki, Yanagihara: "Stimulatory effect of lymphocyte-derived factor on catecholamine efflux form cultured bovine adrenal medullary cells"Neuroscience Letters. 243. 25-28 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yumiko, Toyohira: "Down-regulation of the noradrenaline transporter by interferon-α in cultured bovine adrenal medullary cells"Journal of Neurochemistry. 70. 1441-1447 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhito, Uezono: "Activation of inwardly rectifying KィイD1+ィエD1 channels by GABA-B receptors expression in Xenopus oocytes"Neuroreport. 9. 583-587 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yosuke, Oishi: "Transmural compression-induced proliferation and DNA synthesis through activation of a tyrosine kinase pathway in rat astrocytome RCR-1 cells"Brain Research. 781. 159-166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhito, Uezono: "Adrenomedullin increase intracellular CaィイD12+ィエD1 and inositol 1, 4, 5-trisphosphate in human oligodendroglial cell line KG-1C"Brain Research. 786. 230-234 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reij, Yoshimura: "An active metabolite of carbamazepine, carbamazepine-10, 11-epoxide, inhibits ion channel-mediated catecholamine secretion in cultured bovine adrenal medullary cells"Psychopharmacology. 135. 368-373 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiko, Tanaka: "Pituitary adenylate cyclase-activating polypeptide causes CaィイD12+ィエD1 release from ryanodine/caffeine stores through a novel pathway independent of both inositol trisphosphates and cyclic AMP in bovine adrenal medullary cells"Journal of Neurochemistry. 70. 1652-1661 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiji, Yoshimura: "Carbamazepine-induced up-regulation of voltage-dependent NaィイD1+ィエD1 channels in bovine adrenal medullary cells in culture"The Journal of Pharmacology and Experimental Therapeutics. 287. 411-447 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koji, Kajiwara: "Atherogenic lipoproteins inhibit catecholamine secretion in cultured bovine adrenal medullary cells"Naunyn-Schmiedeberg's Archives of Pharmacology. 358. 308-314 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koji, Hara: "Ketamine interacts with the noradrenaline transporter at a site party overlapping the desipramine binding site"Naunyn-Schmiedeberg's Archives of Pharmacology. 358. 328-333 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyuki, Yanagihara: "Signal transduction of intracellular CaィイD12+ィエD1 in adrenal medullary chromaffin cells"Protein, Nucleic acid and Enzyme. 43・12. 1777-1783 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Megumi, Aso: "cDNA clonig of inducer reactive protein in adult T-cell leukemia cells by applying the fuzzy cloning theory for sequencing defuzzification"Biomedical Soft Computing and Human Science. 5. 15-20 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi, Shibuya: "Prostaglandin EィイD22ィエD2 induces CaィイD12+ィエD1 release from ryanodine/caffeine-sensitive stores in bovine adrenal medullary cells via EPィイD21ィエD2-like"Journal of Neurochemistry. 73. 2167-2174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koji, Kajiwara: "Modification of low density lipoprotein its inhibitory effect on catecholamine secretion in cultured bovine adrenal medullary cells"Naunyn-Schmiedeberg's Archives of Pharmacology. 360. 99-102 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyuki, Yanagihara: "Characterization and functional role leptin receptor in bovine adrenal medullary cells"Biochemical Pharmacology. 59(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiji, Yoshimura: "Inhibitory effects of clozapine and other antipsychotic drugs on noradrenaline transporter in cultured bovine adrenal medullary cells"Psychopharmacology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eiichiro, Nakamura: "ATP activates DNA synthesis through a MAP kinase-dependent pathway by acting on P2X receptors in human osteoblast-like MG-63 cells"The American Journal of Physiology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyuki, Yanagihara: "Factors affecting adrenal medullary catecholamine biosynthesis and release, Catecholamines : Bridging Basic Science with Clinical Medicine"Academic Press. (18-21) 1084 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuyuki, Yanagihara: "Short-term regulation of catecholamine synthesis by CaィイD12+ィエD1/calmodulin-dependent protein kinase II, The Adrenal Chromaffin Cell"Hokkaido University Press. (243-247) (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yumiko, Toyohira: "Elsevier Science"Effects of cytokines on the noradrenaline transporter in cultured bovine adrenal medullary cells, Control Mechanisms of Stress and Emotion : Neuroendocrine-Based Studies. (263-264) 359 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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