1998 Fiscal Year Final Research Report Summary
TGF-β-DPC-4 mediated cell cycle regulation
| Project/Area Number |
09470042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokyo (1998)
Osaka University (1997) |
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Principal Investigator |
AKIYAMA Tetsu Institute of Molecular and Cellular Biosciences, The University of Tokyo, 分子細胞生物学研究所, 教授 (70150745)
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| Project Period (FY) |
1997 – 1998
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| Keywords | DPC4 / TGF-β / Target gene / One-hybrid / Subtraction / Cell Cycle |
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| Research Abstract |
DPC4 (SMAD4) forms a complex with SMAD 2 /SMAD3, and induces transcriptional activation of TGF-beta target genes. We attempted to isolate target genes by yeast one-hybrid system using DPC4 as bait. We screened 2 x 105 clones of a human genome library and isolated three fragments that contain the consensus sequence recognized by DPC4. We searched for the DDBJ, EMBL and GenBank databases and found that one clone is a fragment of the human 28S rRNA gene. We are also trying to isolate DPC4 target genes involved in the regulation of cell cycle progression and signal transduction by the subtraction method and the promoter trap method. To understand the mechanism underlying the DPC4-mediated cell cycle regulation, we are also trying to identify novel DPC4-associated proteins.
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