• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Mechanism of delayed phase of increased vascular permeabitity in acute inflammation

Research Project

Project/Area Number 09470065
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionKumamoto University

Principal Investigator

YOSHINAGA Masaru  Department of Pathology, Kumamoto University School of Medicine, Professor, 医学部, 教授 (90040196)

Project Period (FY) 1997 – 2000
KeywordsINFLAMMATION / INCREASED VASCULAR PERMEABILITY / CYTOKINE / TNFα / IL-8 / IL-1β / IL-1ra / GRO
Research Abstract

To investigate the mechanism of increased vascular permeability (IVP) in acute inflammation, we developed the recombinant cytokines, including interleukin (IL)-1β, tumor necerosis factor-α (TNFα), IL-1receptor antagonist (IL-1a), IL-8, growth stimulating oncogene (GRO), monocyte chemoattractant protein-1 (MCP-1) and their antibodies, together with immunoassay systems. Using rabbits with LPS-induced pleurisy as well as the above mentioned materials and methods, we determined interleukin (IL)-8 as a direct mediator for delayed phase of IVP in acute inflammation on the basis of the following series of evidence ; (1) We reconfirmed that the IVP showed biphasic pattern in the LPS-induced pleurisy similarly to the other types of acute inflammation, and the immediate phase is mediated by histamine. (2) Either blocking of TNFα or IL-8 with corresponding antibodies, resulted in disappearance of the delayed permeability, while the duration and the degree of intensity of immediate permeability was not affected. IL-1ra did not suppress the delayed IVP.(3) The delayed permeability was not observed in neutro-phil-depleted rabbits, but the production of TNFα was maintained. (4) Intrapleural injections of either rabbit recombinant TNFα or IL-8 induced a delayed type of IVP that was not inhibited with antihistamine. (5) Injection of TNFα induced the production of IL-8. (6) IL-8 but not TNFα induced the delayed type IVP in the neutrophil-depleted rabbits. (7) GRO induced a delayed type of IVP via inducing the production of TNFα. Thus, TNFα-induced IL-8 is responsible for the mediation of delayed IVP in LPS-induced pleurisy of rabbits. This mechanism may generally be applicable to many types of acute inflammations, because similar IVP was also observed in inflammations at different site of the body, i.e. articular cavity and vitreal space as well as the inflammation with a different stimuli, i.e. urea crystal in rabbits.

  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Akihiro Matsukawa 他: "Analysis of the inflammatory cytokine network among TNFα, IL-1β, IL-1 receptor antagonist and IL-8 in LPS-induced rabbit arthritis."Laboratory Investigation. 76. 629-638 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiro Matsukawa 他: "IL-1 receptor antagonist in tissues from healthy rabbits and in endotoxemia-related changes. IL-1 receptor antagonist has the potential to regulate over-actions of IL-1 in health and disease."Cytokine. 9. 307-315 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhiko Miyamoto 他: "IL-8 is involved in homologous TNFα-, but not in IL-1β-induced neutrophil infiltration in rabbits."Inflammation Research. 46. 472-477 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumitaka Imamura 他: "Involvement of tumor necrosis factor-α, interleukin-1β, interleukin-8, and interleukin-1 receptor antagonist in acute lung injury caused by local Shwartzman reaction."Pathology International. 47. 16-24 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makoto Kimura 他: "Blocking of TNF-α and IL-1 inhibits leukocyte infiltration at early, but not at late stage of Staphylococcus aureus-induced arthritis and concomitant cartilage destruction in rabbits."Clinical Immunology and Immunopathology. 82. 18-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junsong Mo 他: "Involvement of TNFα, IL-1β and IL-1 receptor antagonist in LPS-induced rabbit uveitis."Experimental Eye Research. 66. 547-557 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi Ujioka 他: "Interleukin-8 as an essential factor in the human chorionic gonadotropin-induced rabbit ovulatory process : Interleukin-8 induces netrophila accumulation and activation in ovulation."Biology of Reproduction. 58. 526-530 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi Ujioka 他: "Analysisof the cytokine interaction among interleukin-1β, interleukin-8 and interleukin-1 receptor antagonist in rabbit ovulatory process."Fertility and Sterility. 70. 759-765 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takumi Fukumoto 他: "IL-8 is an essential mediator of the increased delayed-phase vascular permeability in LPS-induced rabbit pleurisy."Journal of Leukocyte Biology. 63. 584-590 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiro Matsukawa 他: "Analysis of the cytokine network among tumor necrosis factor α, interleukin-1β, interleukin-8 and interleukin-1 receptor antagonist in monosodium urate crystal-induced rabbit arthritis."Laboratory Investigation. 78. 559-569 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiro Matsukawa 他: "Production and regulation of monocyte chemoattractant protein-1 in lipopolysaccharide-or monosodium urate crystal-induced arthritis in rabbits : Roles of tumor necrosis factor α, interleukin-1, and interleukin-8."Laboratory Investigation. 78. 973-985 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiro Matsukawa 他: "Involvement of growth related protein (GRO) in lipopolysaccharide-induced rabbit arthritis : Cooperation between GRO and interleukin (IL)-8, and interrelated regulation among tumor necrosis factor-α, IL-1, IL- 1 receptor antagonist, IL-8 and GRO."Laboratory Investigation. 79. 591-600 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiro Matsukawa 他: "Cytokine network in the joint inflammation."Current Trends in Immunology. 1. 159-167 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junsong Mo 他: "Role and regulation of IL-8 and MCP-1 in LPS-induced uveitis in rabbits."Experimental Eye Research. 68. 333-340 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiro Matsukawa 他: "Neutrophils as a source of cytokines in inflammation."Histology and Pathology. 14. 511-516 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shin-ichi Miyazaki 他: "Neutrophil infiltration as a craucial step for monocyte chemoattractant protein-1 to attract monocytes in lipopolysaccharide-induced arthritis in rabbits."Inflammation Research. 49. 673-678 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jun-Song Mo 他: "CXC chemokine GRO is essential for neutrophil infiltration in LPS-induced uveitis in rabbits."Experimental Eye Research. 70. 221-226 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Takahashi 他: "Erythromycin attenuates an experimental model of chronic bronchiolitis via augmenting MCP-1."European Respiratory Journal. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Matsukawa, T.Yoshimura, K.Miyamoto, S.Ohkawara, and M.Yoshinaga: "Analysis of the inflammatory cytokine network among TNFα, IL-1β, IL-1 receptor antagonist and IL-8 in LPS-induced rabbit arthritis."Lab.Invest.. 76. 629-638 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Matsukawa, T.Fukumoto, T.Maeda, S.Ohkawara, and M.Yoshinaga: "Detection and characterization of IL-1 receptor antagonist in tissues from healthy rabbits : IL-1 receptor antagonist is probably involved in health."Cytokine. 9. 307-315 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Miyamoto, A.Matsukawal, S.Ohkawara, K.Takagi, and M.Yoshinaga: "IL-8 is involved in homologous TNFα-, but not in IL-1β-induced neutrophil infiltration in rabbits."Inflammation Res.. 46. 472-477 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imamura S, Matsukawa A, Ohkawara S, Kagayama M, and Yoshinaga M: "Involvement of tumor necrosis factor-a, interleukin-1b, interleukin-8, and interleukin-1 receptor antagonist in acute lung injury caused by local Shwartzman reaction."Pathology International.. 47. 16-24 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kimura, A.Matsukawa, S.Ohkawara, K.Takagi and M.Yoshinaga: "Blocking of TNF-α. and IL-1 inhibits leukocyte infiltration at early, but not at late stage of Staphylococcus aureus-induced arthritis and concomitant cartilage destruction in rabbits."Clin.Immunol.Immunopathol.. 82. 18-25 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mo, A.Matsukawa, M.Yoshinag: "Involvement of TNFα, IL-1β and IL-1 receptor antagonist in LPS-induced rabbit uveitis."Exp.Eye Res.. 66. 547-557 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ujioka, A.Matsukawa, N.Tanaka, K.Matsuura, M.Yoshinaga and H.Okamura: "Interleukin-8 as an essential factor in the human chorionic gonadotropin-induced rabbit ovulatory process : Interleukin-8 induces netrophila accumulation and activation in ovulation."Biol.Reproduct.. 58. 526-530 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ujioka, A.Matsukawa, N.Tanaka, K.Matsuura, M.Yoshinaga, H.Okamura: "Analysis of the cytokine interaction among interleukin-1β, interleukin-8 and interleukin-1 receptor antagonist in rabbit ovulatory process."Fertility and Sterility. 70. 759-765 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Fukumoto, , A.Matsukawa, T.Yoshimura, S.Edamitsu, S, Ohkawara, K.Takagi. and M.Yoshinaga: "IL-8 is an essential mediator of the increased delayed-phase vascular permeability in LPS-induced rabbit pleurisy."J.Leuko.Biol.. 63. 584-590 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Matsukawa, T.Yoshimura, T.Maeda, T.Takahashi, S.Ohkawara and M.Yoshinaga: "Analysis of the cytokine network among tumor necrosis factor α, interleukin-1β, interleukin-8 and nterleukin-1 receptor antagonist in monosodium urate crystal-induced rabbit arthritis."Lab.Invest.. 78. 559-569 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Matsukawa, S.Miyazaki, T.Maeda, S.Tanase, L.Feng, S.Ohkawara, M.Yoshinaga and T.Yoshimura: "Production and regulation of monocyte chemoattractant protein-1 in lipopolysac-charide-or monosodium urate crystal-induced arthritis in rabbits : Roles of tumor necrosis factor a, interleukin-1, and interleukin-8."Lab.Invest.. 78. 973-985 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Matsukawa, T.Yoshimura, K.Fujiwara, T.Maeda, S.Ohkawara and M.Yoshinaga: "Involvement of growth related protein (GRO) in lipopolysaccharide-induced rabbit arthritis : Cooperation between GRO and interleukin (IL)-8, and interre-lated recrulation among tumor necrosis factor-a, IL-1, IL-1 receptor antagonist, IL-8 and GRO."Lab.Invest.. 79. 591-600 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsukawa A and Yoshinaga M: "Cytokine network in the joint inflammation."Current Trends in Immunol. 1. 159-167 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Mo, A.Matsukawa, S.Ohkawawa and M.Yoshinaga: "Role and regulation of IL-8 and MCP-1 in LPS-induced uveitis in rabbits."Exp.Eye Res.. 68. 333-340 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Matsukawa and M.Yosinaga: "Neutrophils as a source of cytokines in inflammation."Histol.Pathol.. 14. 511-516 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Furue, K.Kuwabara, K.Mikawa, K.Nishina, M.Shiga, N.Maekawa, M.Ueno, Y.Chikazawa, T.Ono, Y.Hori, A Matsukawa, M.Yoshinaga and H.Obara: "Crucial role of group IIA phospholipase A2 in oleic acid-induced acute lung injury in rabbits."Am.J.Repri.Crit.Care Med.. 160. 1291-1302 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyazaki, S, Matsukawa A, Ohkawara S, Takagi K and Yoshinaga M: "Neutrophil infiltration as a craucial step for monocyte chemoattractant protein-1 to attract monocytes in lipopolysaccharide-induced arthritis in rabbits."Inflam.Res.. 49. 673-678 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Mo, A.Matsukawa, S.Ohkawara and M.Yoshinaga: "CXC chemokine GRO is essential for neutrophil infiltration in LPS-induced uveitis in rabbits."Exp.Eye res.. 70. 221-226 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shibata, M, Uno, T, Kajihara, M, Ohkawara, S and Yoshinaga, M: "Enhanced LPS-fever following hyperthermic stress in rabbits."J.Thermal Biology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi T., Suga M., Matsukawa A., Sato K., Okamoto T., Ichiyasu H., Ohkawara S., Yohsinaga M.and Ando M.: "Eryth-romycin attenuates an experimental model of chronic bronchiolitis via augmenting MCP-1."Eur.Resp.J.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi