1997 Fiscal Year Annual Research Report

ストレス蛋白質に対するT細胞応答の分子機構

Research Project

Project/Area Number	09470067
Research Institution	Sapporo Medical University
Principal Investigator	佐藤昇志札幌医科大学, 医学部, 助教授 (50158937)
Co-Investigator(Kenkyū-buntansha)	菊地浩吉札幌医科大学, 医学部, 教授 (00045345)
Keywords	hsp 70 / TCR / γδ T cell / ハイブリドーマ
Research Abstract	本年度は70kDa hspと反応するT細胞レセプター(TCR)構造を決定することができた。すなわち、CD3^+、CD4^-、CD8^-、NKR-P1^-、TRCαβ^-のT細胞とBW5147細胞のT cell hybridomasを得た。これらのTCR構造を調べたところいずれもTCRγ2δ6であった。CDR-3の部分を検索するといずれのクローンにも6つのamino acid stretchをみることができたが、アミノ酸構成は異なりN-diversityをみとめることができた。すなわちfamily usageは同じであったがCDR-3は異なっていた。このことは70kDa hsp様分子が提示分子として働くとしてもこれと複合体をつくる物質にある程度のheterogeneityの存在することが推測された。

Research Products
(8 results)

All Other

All Publications (8 results)

[Publications] Takashima,S.,Sato,N.,Kikuchi,K.et al: "Involvement of peptide antigens in the cytotoxicity between 70 kDa heat shock cognate protein-like molecule and CD3^+,CD4^-,CD8^-,T cell receptor α/β^-killer T cells." J.Immunol.157. 3391-3395 (1996)
[Publications] Fujita,T.,Takahashi,S.,Sato,N.et al: "Prolonged survival of rat skin allograft by treatment with FK506 ointment." Transplantation. 64. 922-925 (1997)
[Publications] Miyazaki,A.,Sato,N.,Kikuchi,K.et al: "Cytotoxicity of histocompatibility leukocyte antigen-DR8-restricted CD4_+ killer T cells against human autologous squamous cell carcinoma." Jpn.J.Cancer Res.88. 191-197 (1997)
[Publications] Tsuruma,T.,Yagihashi,A.,Torigoe,T.,Sato,N.,Kikuchi,K and Hirata,K.: "Interleukin-10 reduces natural killer sensitivity of tumor cells." Cell.Immunol.(in press).
[Publications] Kamiguchi,K.,Torigoe,T.,Sato,N.,Kikuchi,K.et al: "73kD heat shock cognate protein is associated with transporter associated with antigen processing." Int.Immunol.(in press).
[Publications] Hirai,I.,Sato,N.,Kikuchi,K.et al: "Localization of pNT22 70kDa heat shock cognate-like protein in the plasma membrane." Exp.Cell Res.(submitted).
[Publications] 佐原弘益、佐藤昇志、高橋延昭、菊地浩吉: "癌拒絶抗体ペプチドによる治療" 中外医学社, 216-225 (1998)
[Publications] Sato,N.,Torigoe,T.: "The molecular chaperones in the cell cycle control.In:Stress of life from molecules to man.ed.:P.Csermely,Annals of New York Academy of Sciences,Vol.851," (in press), (1998)

1997 Fiscal Year Annual Research Report

ストレス蛋白質に対するT細胞応答の分子機構

Principal Investigator

佐藤 昇志 札幌医科大学, 医学部, 助教授 (50158937)

Research Products

[Publications] Takashima,S.,Sato,N.,Kikuchi,K.et al: "Involvement of peptide antigens in the cytotoxicity between 70 kDa heat shock cognate protein-like molecule and CD3^+,CD4^-,CD8^-,T cell receptor α/β^-killer T cells." J.Immunol.157. 3391-3395 (1996)

[Publications] Fujita,T.,Takahashi,S.,Sato,N.et al: "Prolonged survival of rat skin allograft by treatment with FK506 ointment." Transplantation. 64. 922-925 (1997)

[Publications] Miyazaki,A.,Sato,N.,Kikuchi,K.et al: "Cytotoxicity of histocompatibility leukocyte antigen-DR8-restricted CD4_+ killer T cells against human autologous squamous cell carcinoma." Jpn.J.Cancer Res.88. 191-197 (1997)

[Publications] Tsuruma,T.,Yagihashi,A.,Torigoe,T.,Sato,N.,Kikuchi,K and Hirata,K.: "Interleukin-10 reduces natural killer sensitivity of tumor cells." Cell.Immunol.(in press).

[Publications] Kamiguchi,K.,Torigoe,T.,Sato,N.,Kikuchi,K.et al: "73kD heat shock cognate protein is associated with transporter associated with antigen processing." Int.Immunol.(in press).

[Publications] Hirai,I.,Sato,N.,Kikuchi,K.et al: "Localization of pNT22 70kDa heat shock cognate-like protein in the plasma membrane." Exp.Cell Res.(submitted).

[Publications] 佐原弘益、佐藤昇志、高橋延昭、菊地浩吉: "癌拒絶抗体ペプチドによる治療" 中外医学社, 216-225 (1998)

[Publications] Sato,N.,Torigoe,T.: "The molecular chaperones in the cell cycle control.In:Stress of life from molecules to man.ed.:P.Csermely,Annals of New York Academy of Sciences,Vol.851," (in press), (1998)

佐藤昇志札幌医科大学, 医学部, 助教授 (50158937)