1998 Fiscal Year Final Research Report Summary
Molecular and Cellular Analysis of a Leucine-rich Repeat Molecule RP105
Project/Area Number |
09470096
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Immunology
|
Research Institution | Saga Medical School |
Principal Investigator |
MIYAKE Kensuke Saga Medical School, Associated Professor, 医学部, 助教授 (60229812)
|
Project Period (FY) |
1997 – 1998
|
Keywords | Blymphocytes / RP105 / leucine-rich repeat / knockout mice / gene cloning |
Research Abstract |
RP1O5 is a LRR molecule that is expressed on murine B lymphocytes. Its cross-linking with a monoclonal antibody delivers an activation signal that leads to resistance against apoptosis and massive proliferation in B cells. The following results were obtained in this term. 1. Molecular cloning of MD-1, a molecule which is associated with RP1O5. A molecule that is coprecipitated with RP1O5 was purified with the anti-RP1O5 antibody and amino acid sequence was determined. With the sequence, a cDNA clone was isolated. The molecule MD-1 is a secretory molecule but binds to the extracellular LRR of RP1O5. 2. The signaling pathways RP1O5 employs were examined using mice lacking signaling molecules. B cells lacking lyn, btk, Erk-specific MAP kinase, protein kinase C betaI/II, c-rel, nfkb1(p105) did not respond to the anti-RP1O5 antibody, indicating the presence of multiple signaling pathways downstream of RP1O5. There results were obtained by collaboration with Drs. S.Gerondakis (Australia) and A.Tarakhovsky (Germany). 3. A monoclonal antibody against human RPIO5 was established. Human RP1O5 was expressed on B lymphocytes, associated with human MD-1, and transmits an activation signal. 4. Mice lacking RP1O5 were established and now under investigation.
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Research Products
(19 results)