2000 Fiscal Year Final Research Report Summary
Molecular epidemiological study on relationship between alcohol metabolizing Enzyme and alcohol-related diseases
| Project/Area Number |
09470105
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Kurume University |
|---|
Principal Investigator |
FUKUDA Katsuhiro Kurume University, School.Med.Dep.Public Health, Professor, 医学部, 教授 (60045416)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OGIMOTO Itsuro Kurume University, School.Med.Dep.Public Health, Lecturer, 医学部, 講師 (30177159)
NAKAMURA Jun Univ.Occup.& Env.Hlth.School.Med.Dep.Psychiatry, Professor, 教授 (40148804)
SHIBATA Akira Kurume University, School.Med.Dep.Public Health, Associate Professor, 医学部, 助教授 (10113226)
SAKATA Ritsu Kurume University, School.Med.Dep.Public Health, Instructor, 医学部, 助手 (60258423)
NISHIYORI Atushi Kurume University, School.Med.Dep.Public Health, Instructor, 医学部, 講師 (30218226)
|
|---|
| Project Period (FY) |
1997 – 2000
|
| Keywords | Alcohol use disorder / PCR-SSCP / ADH2 / ALDH2 / AUDIT / heavy drinking / primary hepatocellular carcinoma |
|---|
| Research Abstract |
(1) An accurate and easy genotyping method of alcohol dehydrogenase2 (ADH2) and aldehyde dehydrogenase2 (ALDH2) by PCR-SSCP has been developped using nail clippings.
(2) The alcohol disorder identification test developed by WHO (AUDIT) has been modified and translated into Japanse (K/AUDIT) which can be applicable for use in the northern Kyushu area in Japan. Based on psychiatry-specialist-confirmed 106 alcohol use disorder (AUD) patients and non-AUD patients with other diseases, the K/AUDIT's criterion-related-validity has been examined. Applying score 12 as the cut-off score to them, the proportion of correct screening of AUD was 0.939, with κ statistics, sensitivity and specificity being 0.877, 0.915 and 0.962, respectively.
(3) Applying the PCR-SSCP method for ADH2 and ALDH2 genotyping, a case-control study on 106 AUD-non-AUD pairs was performed and revealed that a person with ADH2^</1> or a person with ALDH2^</1> showed 3.5 (P<0.05) or 7.1 (P<0.05) times likely to develop AUD than those with other genotypes, respectively.
(4) A case-control study performed on primary hepatocellular carcinoma (HCC) in Fukuoka and Saga Prefecture produced 177 male HCC cases, and age-, sex-, residence-matched hospital-based controls. Data e.g. HBsAg, anti-HCVAb, life-style infomations e.g. smoking or drinking havits have been collectd by a structured interview with nail crippings. Univariate analysis using logistic regression model revealed that factors ; HbsAg positive, anti-HCVAb positive, heavy drinking (average drinking amount by age 40 was 60 drink-years or more) and ALDH2^</1> homozygote were statistically significant risk factors for developing male HCC, respectively.
|
