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1999 Fiscal Year Final Research Report Summary

Forensic Studies on Identification of a Suspect in a Sexual Crime by Typing of DNA Polymorphisms and Biochemical Genetic Markers

Research Project

Project/Area Number 09470122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionGunma University

Principal Investigator

YASUDA Toshihiro  Gunma University School of Medicine, Department of Legal Medicine, Associate Professor, 医学部, 助教授 (80175645)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Tamiko  Gunma University School of Medicine, Department of Legal Medicine, Assistant, 医学部, 助手 (40008561)
TAKESHITA Haruo  Gunma University School of Medicine, Department of Legal Medicine, Lecturer, 医学部, 講師 (90292599)
KISHI Koichiro  Gunma University School of Medicine, Department of Legal Medicine, Professor, 医学部, 教授 (30169841)
Project Period (FY) 1997 – 1999
KeywordsPersona identification / Sexual crime / Deoxyribonuclease / Semen / Genetic polymorphism / Molecular biology / Human genetics / Genetic markers
Research Abstract

1. We have previously found human deoxyribonuclease (DNase) II to be a genetic marker present in semen. In this study, we succeeded in cloning of both the complementary and genomic DNAs encoding human DNase II. Furthermore, regional localization of the gene (DNASE2) to 19p13.2-p13.1 was achieved by FISH analysis. This is the first report of the cloning and characterization of the cDNA and gene for mammalian DNase II.
2. DNase II levels in human vary depending on whether the individual has the DNASE2ィイD1*ィエD1H or ィイD1*ィエD1L allele. The transient transfection luciferase analysis of the DNase II gene expression permit us to conclude that the A-75G transition in the proximal promoter region causes the allelic difference in the promoter activity of the gene, underlying its genetic polymorphism.
3. Human DNase II was composed of three non-identical subunits. From site-directed mutagenesis study, it was found that a simultaneous attachment of a carbohydrate moiety, proteolytic cleavage and the … More inherent signal peptide might be required for subcellular sorting and maturation of the enzyme.
4. Since two new alleles, DNASEIィイD1*ィエD15 and ィイD1*ィエD16, of human DNase I polymorphism were identified, DNase I-polymorphism can be classified into 21 different phenotypes. These findings permit the ability of DNase I-polymorphism in personal identification to be further elevated. The type 6 isoenzyme was the first type to be shown to be labile.
5. The findings that Caucasian and Negroid populations were classified into several DNase I phenotypes found DNase -polymorphism to be world-wide. Furthermore, we found a geographical north-south decline in DNase I 2 allele frequency in Japanese populations.
6. Two short tandem repeat systems, HumTPO and HumLPL, were investigated in Japanese populations by means of newly developed genotyping method. Duplex typing of these systems were found to be very powerful in forensic utility.
Considering these findings made in this research project, both DNases I and II have been confirmed to be one of the most useful and effective genetic markers suited for personal identification from forensic semen samples. Less

  • Research Products

    (86 results)

All Other

All Publications (86 results)

  • [Publications] Toshihiro Yasuda: "Purification of deoxyribonuclease I(DNase I) using two-step chromatography"Analytical Biochemistry. 245(2). 255-257 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aoyama Masahiro: "DYS19 marker in Japanese populations"International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Population studies of human deoxyribonuclease I polymorphism"Human Heredity. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Mouse deoxyribonuclease I(DNase I)"Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Rabbit deoxyribonuclease I(DNase I)"Biochemical Journal. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Iida: "The fifth allele of the human deoxyribonuclease I(DNase I) polymorphism"Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Souichi Tsutsumi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma"Cancer. 82(9). 1621-1625 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamiko Nakajima: "Two novel sereening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity"Immunological Investigation. 27(3). 145-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Structure and organization of the human deoxyribonuclease II (DNase II) gene"Annals of Human Genetics. 63(4). 299-305 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Shimada: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine"The Journal of Histochemistry and Cytochmistry. 46(7). 833-840 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Iida: "Detection of isoenzymes of deoxyribonucleases I and II electrophoresed gels with picogram sensitivity using SYBR Green I"Electrophoresis. 19(14). 299-305 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Identification of the three non-identical subunits constituting human deoxyribonuclease II"FEBS Letters. 440(1/2). 239-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "The STR loci HumTPO and HumLPL : population genetic data in eight populations"International Journal of Legal Medicine. 110(6). 331-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Hosomi: "Simple purification method of the anriphospholpied antibody from normal human plasma"Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on SYBR Green I fluorescence"Analytical Biochemistry. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Molecular cloning of the cDNA encoding human deoxyribonuclease II"The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chaine reaction and fluorescence in situ hybridization analysis"Biochemical and Biophysical Research Communications. 244(3). 815-818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Detection of the two short tandem repeat loci (HumTOP and HumLPL) in Japanese populations using discontinuous polyacrylamid gel electrophoresis"The Japanese Journal of Legal Medicine. 52(2). 139-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Structural requirement of a human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis"Biochemical and Biophysical Research Communications. 256(3). 591-594 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Hosomi: "Xenopus lavis pancreatic DNase I"Experimental and Clinical Immunogenetics. 16(2). 117-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "A new allele, DNASE1^*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Research Communications. 260(1). 280-283 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimitsu Nakashima: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamiko Nakajima: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney : fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 133-142 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Activity staining for detection of ribonucleases using the dried agarose film overlay method after electrophoresis"Methods in Enzymology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequence. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Geographical north-south decline in DNase I allele 2 in Japanese populations"Human Biology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岸 紘一郎: "核酸分解酵素DNaseIの遺伝的多型の発見"医学のあゆみ. 180(10). 658-659 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安田 年博: "ヒトdeoxyribonuclease II(DNase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岸 紘一郎: "DNaseI"日本臨床. 57(増刊). 486-489 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 飯田 礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位"DNA多型. Vol.5. 203-206 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 飯田 礼子: "PCR-SSCP法によって検出されたdeoxyribonuclease I遺伝子の新規なミスセンス差異"DNA多型. Vol.6. 132-135 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹下 治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. Vol.7. 88-90 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane : a case study and animal experiment"International Journal of Legal Medicine. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Genetic polymorphisms detected in human urine : their appolication to forensic individualization"The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Yasuda: "Anthropological applications of genetic polymorphisms detected in human urine : a review of Japanese studies"Revista di Antropologia. 75(1). 407-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 灘野 大太: "アポトーシスとエンドヌクレアーゼとの関連性"臨床薬理の進歩. No.18. 105-110 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安田 年博: "ヒト脳下垂体には高活性なdeoxyribonuclease Iが存在する"日本法医学雑誌. 52(2). 295-300 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤川 克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹下 治男: "DNASE1^*6遺伝子がつくる酵素の寿命は短い(印刷中)"DNA多型. Vol.8.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 飯田 礼子: "年齢依存性を示すマウス腎の新規な遺伝子検索(印刷中)"DNA多型. Vol.8.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruo Takeshita: "Mammlian deoxyribonucleases I are classified into three types : pancreas, parotid and pancreas-parotid(mixed) types, based on differences of their tissue concentrations (in press)"Biochemical and Biophysical Research Communications.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Purification of deoxyribonuclease I (DNase I) using two-step chromatography"Anal. Biochem.. 245(2). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Aoyama, T. Matsuki, K. Kishi, R. Iida, E. Tsubota.: "DYS19 marker in Japanese populations"Int. J. Legal Med.. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, I. Yuasa, K. Kishi.: "Population studies of human deoxyribonuclease I polymorphism"Hum. Hered.. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Mouse deoxyribonuclease I (DNase I) : biochemical and immunological characterizations, cDNA structure and tissue distribution"Biochem. Mol. Biol. Int.. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Rabbit deoxyribonuclease I (DNase I) : purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships with other DNases I and phylogenetic analysis"Biochem. J.. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Iida, T. Yasuda, M. Aoyama, E. Tsubota, M. Kobayashi, I. Yuasa, K. Kishi.: "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism"Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, E. Meyer, B. Brinkmann.: "The STR loci HumTPO and HumLPL : population genetic data in eight populations"Int. J. Legal Med.. 110(6). 331-333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O. Hosomi, A. Takeya, T. Yasuda, S. Yazawa, T. Nakajima, T. Kogure, H. Iwai, K. Kishi.: "Simple purification method of the antiphospholipid antibody from normal human serum"Exp. Clin. Immunogenet.. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, E. Nakazato, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence"Anal. Biochem.. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Molecular cloning of the cDNA encoding human deoxyribonuclease II"J. Biol. Chem.. 273(5). 2610-1616 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, T. Nakajima, O. Hosomi, Y. Nakashima, K. Mogi, K. Kishi.: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis"Biochem. Biophys. Res. Commun.. 244(3). 815-818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, O. Hosomi, Y. Nakashima, S. Tsutsumi, K. Kishi.: "Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis"Jpn. J. Legal Med.. 52(2). 139-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Tsutsumi, T. Asao, Y. Nagamachi, T. Nakajima, T. Yasuda, K. Kishi.: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma"Cancer. 82(9). 1621-1625 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Nakajima, T. Yasuda, Y. Nakashima, O. Hosomi, H. Takeshita, K. Kishi.: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity"Immunol. Invest.. 27(3). 145-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, S. Tsutsumi, T. Nakajima, O. Hosomi, S. Mori, K. Kishi.: "Structure and organization of the human deoxyribonuclease II (DNase II) gene"Ann. Hum. Genet.. 62(4). 299-305 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O. Shimada, H. Ishikawa, H. Shimada, T. Yasuda, K. Kishi, S. Suzuki.: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine"J. Histochem. Cytochem. 46(7). 833-840 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Iida, T. Yasuda, E. Tsubota, Y. Nakashima, K. Sawazaki, M. Aoyama, T. Matsuki, K. Kishi: "Detection of isoenzymes of deoxyribonucleases I and II on electrophoresed gels with picogram sensitivity using SYBR Green I"Electrophoresis. 19(14). 299-305 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, T. Yasuda, R. Iida, T. Nakajima, O. Hosomi, Y. Nakashima, S. Mori, K. Kishi.: "Identification of the three non-identical subunits constituting human deoxyribonuclease II"FEBS Lett.. 440(1/2). 239-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Iida, K. Sawazaki, T. Yasuda, E. Tsubota, M. Aoyama, T. Matsuki, K. Kishi.: "Deoxyribonuclease I phenotyping from saliva stains"J. Forensic Sci.. 44(1). 175-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, T. Nakajima, O. Hosomi, Y. Nakashima, S. Mori, K. Kishi.: "Structural requirement of a human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis"Biochem. Biopjys. Res. Commun.. 256(3). 591-594 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] O. Hosomi, T. Yasuda, H. Takeshita, T. Nakajima, Y. Nakashima, Y. Hanaoka, K. Kishi.: "Xenopus laevis pancreatic DNase I : purification and characterization"Exp. Clin. Immunogenet.. 16(2). 117-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, S. Kogure, K. Kishi.: "A new allele, DNASEィイD1*ィエD16, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochem. Biophys. Res. Commun.. 260(1). 280-283 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis"Legal Med.. 1(3). 233-237 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Nakashima, T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, S. Mori, K. Kishi.: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I"Int. J. Biochem. Cell Biol.. 31(11). 1313-1324 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Iida, T. Yasuda, E. Tsubota, T. Matsuki, K. Kishi.: "Age-related changes of gene expression in mouse kidney : fluorescence differential display-PCR analysis"Mech. Aging Develop.. 113(2). 135-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, E. Nakazato, T. Nakajima, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Lett.. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Nakajima, T. Yasuda, H. Takeshita, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Exp. Clin. Immunogenet.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, O. Hosomi, H. Takeshita, T. Nakajima, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequence. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Nakashima, T. Yasuda, H. Takeshita, K. Mogi, K. Kishi, H. Shiono, K. Sagisaka, I. Yuasa, H. Nishimukai, H. Kimura.: "Geographical north-south decline in DNase I allele 2 in Japanese populations"Hum. Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, K. Mogi, T. Yasuda, S. Mori, Y. Nakashima, T. Nakajima, H. Akuzawa, S. Nakajo, Y. Hirota, K. Kishi.: "Postmortem absorption of dichloromethane : a case study and animal experiments"Int. J. Legal Med.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, K. Mogi, T. Yasuda, T. Nakajima, S. Mori, T. Hoshino, K. Kishi.: "Mammalian deoxyribonuclease I are classified into three types."Biochem. Biophys. Res. Commun. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, H. Takeshita, K. Kishi.: "Genetic polymorphisms detected in human urine : their application to forensic individualization"Jpn. J. Legal Med.. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda, D. Nadano, H. Takeshita, R. Iida, K. Kishi.: "Anthropological applications of genetic polymorphisms detected in human urine : a review of Japanese studies."Rivista di Antropologia. 75(1). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda: "High activity of deoxyribonuclease I identified in human pituitary gland-structure and function"Jpn. J. Legal Med.. 52(2). 295-300 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Fujikawa, A. Ishijima, T. Hattori, T. Yasuda, K. Kishi.: "Geographical cline of transferrin polymorphism in Japanese (especially in Saitama and Aomori prefectures)"Blood Programme. 21(4). 279-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kishi, T. Yasuda.: "Discovery of genetic polymorphism of deoxyribonuclease I"Igaku no Ayumi. 180(10). 658-659 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yasuda: "Recent study on human deoxyribonuclease II"Kitakanto Med. J.. 49(1). 39-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Kishi, T. Yasuda, H. Takeshita.: "DNase I"Nihon Rinsyo. 57(Special Issue). 486-489 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Iida, M. Aoyama, T. Matsuki, T. Yasuda, K. Kishi.: "Detection of a new polymorphic site in intron 7 of the human deoxyribonuclease I gene by PCR-SSCP analysis"DNA Polymorphisms. Vol.5. 203-206 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Iida, M. Aoyama, T. Matsuki, T. Yasuda, K. Kishi.: "A new missense mutation detected in the human deoxyribonuclease I gene by PCR-SSCP analysis"DNA Polymorphisms. Vol.6. 132-135 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, K. Kishi.: "Simultaneous detection of the STR loci vWF and HumTPO using discontinuous polyacrylamide gel electrophoresis"DNA Polymorphisms. Vol.7. 88-90 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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