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1999 Fiscal Year Final Research Report Summary

Uroguanylin's functions in an endocrine, parauine and luminocrine fashion

Research Project

Project/Area Number 09470140
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionMiyazaki Medical College

Principal Investigator

NAKAZATO Masamitsu  Miyazaki Medical College, Third Department of Internal Medicine, Full time Lecturer, 医学部, 講師 (10180267)

Co-Investigator(Kenkyū-buntansha) MIYAZATO Mikiya  National Cardiovascular Center Research Institute, Department of Biochemistry, Assistant Director, 生化学部, 室員 (50291183)
MATSUKURA Shigeru  Miyazaki Medical College, Third Department of Internal Medicine, Professor, 医学部, 教授 (70030939)
Project Period (FY) 1997 – 1999
Keywordsuroguanylin / guanylin / cyclic GMP / guanylate cyclase / salt and water metabolism
Research Abstract

Uroguanylin, a member of the guanylin peptide family, acts on guanylyl cyclase C (GC-C) to regulate intestinal and renal fluid and electrolyte transport through the second messenger, cyclic GMP. Using an antiserum raised against synthetic rat uroguanylin, we established a radioimmunoassay and identified three endogenous molecular forms in the intestine and kidney: a 15-amino acid uroguanylin, an 18-amino acid uroguanylin that is a monobasic processing product, and a 9.4-kDa prouroguanylin. Prouroguanylin is the major molecular form in these two tissues, whereas only 15-amino-acid uroguanylin is present in the urine. Rat uroguanylin is most abundant in the proximal small intestine, its content decreasing toward the colon. Uroguanylin is present immunohistochemically in the endocrine cells in the intestine and stomach, B cells in the pancreatic islets, and tubular epithelial cells in the kidney. Uroguanylin has a widespread tissue distribution and is located in cells which function in an endocrine, paracrine and/or immunocrine (luminal secretion) fashion. Uroguanylin may have physiological functions other than the regulation of fluid and electrolyte transport in the intestine and kidney.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Fujimoto s, Kinoshita H, Hara S, Nakazato M, Hisanaga S, Eto T: "Immunohistochemical localization of uroguanylin in the human kidney"Nephron. 84. 88-89 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukae H, Kinoshita H, Fujimoto S, Nakazato M, Eto T: "plasma concentration of uroguanylin in patients on maintenance dislysis therapy"nephron. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Date Y, Nakazato M, Yamaguchi H, Kangawa K, Kinoshita Y, Chiba T, Ueta Y, Yamashita H, Matsukura S: "Enterochromaffin-like cell, a cellular source of uroguanylin in rat stomach"Endocrinology. 140. 2398-2404 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita H, Fujimoto S, Fukae H, yokota N, Hisanaga S, Nakazato M, Eto T: "Plasma and urine levels of uroguanylin, a new natriuretic peptide, in nephrotic syndrome"Nephron. 81. 160-164 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chino N, Kubo S, Kitani T, yoshida T, Tanabe R, Kobayashi Y, nakazato M, Kangawa K, Sakakibara S.: "Topological isomers of human uroguanylin: Interconversion between biologically active and inactive isomers"FEBS Lett. 421. 27-31 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazato m, Yamaguchi H, Date Y, kangawa k, Goy M, Chino N, matsukura S: "Tissue distribution, cellular source and structural analysis of rat immunoreactive uroguanylin"Endocrinology. 139. 5247-5254 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita H, Nakazato M, Fujimoto s, Eto T: "Biochemical Res"Significance of uroguanylin, a new natriuretic peptide in renal diseases. 255-258 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中里雅光: "消化管ホルモン"水・NaCl代謝調節に作用する新しい消化管ペプチドグアニリンハァミリー. 9-14 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita H, Nakazato M, Fujimoto S, Eto T: "Significance of uroguanlin, a new natriuretic peptide, in renal diseases. Current Topics in Biochemical Research"RESEARCH TRENDS Biochemical Res.. 1. 255-258

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakazato M, Yamaguchi H, Date Y, Kangawa K, Goy M, Chino N, Matsukura S: "Tissue distribution, cellular source and structural analysis of rat immunoreactive uroguanylin."Endocrinology. 139. 5247-5254 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chino N, Kubo S, Kitani T, Yoshida T, Tanabe R, Kobayashi Y, Nakazato M, Kangawa K, Sakakibara S,: "Topological isomers of human uroguanylin: Interconversion between biologically active and inactive isomers."FEBS Lett. 421. 27-31 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Date Y, Nakazato M, Yamaguchi H, Kangawa K, Kinoshita Y, Chiba T, Ueta Y, Yamashita H, Matsukura S: "Enterochromaffin-like cell, a cellular source of uroguanylin in rat stomach."Endocrinology. 140. 2398-2404 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita H, Fujimoto S, Fukae H, Yokota N, Hisanaga S, Nakazato M, Eto T: "Plasma and urine levels of uroguanylin, a new natriuretic peptide, in nephrotic syndrome."Nephron. 81. 160-164 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimoto S, Kinoshita H, Hara S, Nakazato M, Hisanaga S, Eto T.: "Immunohistochemical localization of uroguanylin in the human kidney"Nephron. 84. 88-89 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukae H, Kinoshita H, Fujimoto S, Nakazato M, Eto T: "Plasma concentration of uroguanylin in patients on maintenance dialysis therapy"Nephron. 84. 206-210 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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