| Co-Investigator(Kenkyū-buntansha) |
OHI Hiroaki SHOWA UNIVERSITY, SCHOOL OF PHARMACRUTICAL SCIENCE, LECTURER, 薬学部, 講師 (60194065)
SUNADA Yoshihide KAWASAKI MEDICAL SCHOOL, DEPARTMENT OF NEUROLOGY, PROFESSOR, 医学部, 教授 (00240713)
MATSUMURA Kiichiro TEIKYO UNIVERSITY, DEPARTMENT OF NEUROLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (50260922)
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| Research Abstract |
Caveolin-3 is the muscle-specific isoform of the caveolin protein family, which is a major component of caveolae, small membrane invaginations found in most cell types. Caveolins play important roles in the formation of caveola membranes, acting as scaffolding proteins to organize and concentrate lipid-modified signaling molecules, and modulate a signaling pathway. For instance, caveolin-3 interacts with neuronal nitric-oxide synthase (nNOS) and inhibits its catalytic activity. Via the syntrophin-nNOS linkage, caveolin-3 also interacts with the dystroglycan complex, which is a major player of muscle cell adhesion to laminin. Recently, specific mutations in the caveolin-3 gene, including the Pro104Leu missense mutation, have been shown to cause an autosomal dominant limb-girdle muscular dystrophy (LGMD1C), which is characterized by the deficiency of caveolin-3 in the sarcolemma. However, the molecular mechanism by which these mutations cause the deficiency of caveolin-3 and muscle cell degeneration remains elusive. Here we generated transgenic mice expressing the Pro104Leu mutant caveolin-3. They showed severe myopathy accompanied by the deficiency of caveolin-3 in the sarcolemma, indicating a dominant negative effect of mutant caveolin-3. Interestingly, we found a great increase of nNOS activity in their skeletal muscle, which, we propose, may play a role in muscle fiber degeneration in caveolin-3.
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