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1998 Fiscal Year Final Research Report Summary

The role of mossy fiber sprouting, neuronal death and glial activation in epileptogenesis

Research Project

Project/Area Number 09470207
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionTohoku University

Principal Investigator

SATO Mitsumoto  Tohoku University Medicine Professor, 医学部, 教授 (70033321)

Co-Investigator(Kenkyū-buntansha) NUMACH Yotaro  Tohoku University Department of Psychiatry Lecturer, 医学部・附属病院, 講師 (00261636)
MATSUOKA Hiro  Tohoku University Medicine Associate Professor, 医学部, 助教授 (00173815)
Project Period (FY) 1997 – 1998
KeywordsKindling / mossy fiber sprouting / neuronal death / hippocampus / glia / neuroqenesis
Research Abstract

Hippocampal sclerosis (HS) is the most common neuropathological change which frequently observed in temporal lobe epilepsy. The major neuropathology of HS is neuronal loss, gliosis and mossy fiber sprouting (MFS) in the hippocampus. Recent experimental evidence suggests the origin of MFS.Neuronal loss in the hilar polymorphic neuron leads aberrant axonal sprouting from preexisting mature dentate granule cells in to the inner molecular layer. And another suggest that neurogenesis in dentate gyms was increased by brain insult that newly born granule cells project axons in to inner molecular layer.
The main objective of the present study is to clarify the origin of MFS using daily amygdala kindling. For this purpose, first we consider when MFS occurs, next using in situ terminal deoxynucleotigyltransferasemediated dUTP nick-end labeling (TUNEL) method to detect neuronal death in the dentate gyrus, and to detect neurogenesis of granule cells using bromodeoxyuridine (BrdU), and finally using immunohistological methods to examine the activation of glial cells which may participate with axonal sprouting.
The result were the kindling develop without MFS or neuronal death in hilus region. There were significant TUNEL positive cells after repetition of 18 afterdischarges or 10 generalize convulsions. Astrocyte was activated at the stage when sprouting occur and microglia was activated at the stage when TUNEL positive neuron was significant in granular cell layer. The degree of neurogenesis of granule cell correlate with the severity of seizure.
From these result it may suggest MFS originate from newly born granule cells after kindling developed and astrocyte may participate with axonal sprouting.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Onodera K: "Notor behavioral function for histamine-dopamine interaction in brain" Inflamm res. 47 suppl.S30-31 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imran MB: "Use of automatized image registration to penetrate mean brain SPECT image of Alzheimer′s patient" Ann Nucl Med. 12. 127-132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyota H: "Decreased central histamine in the amygdaloid kindling rats." Brain Research. 802. 241-246 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida S: "Impairment of diff avoidance reaction induced by subchronic methamphetomine administration and restraiutstress : compotisou between two imbred straivs of rats" Prog Neuro-psydropharmacol Bio Psychiat. 22. 1023-1032 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sheu, H: "A lasting change in trazodone response after non-convulsive electroshock therapy for medication-resistant senile depression" Psy chiatry and Clin Neurosci. 52. 111-113 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 光源: "キンドリングからみた難治てんかんの神経機構" 日本神経 精神薬理学雑誌. 17. 31-34 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 光源: "てんかん研究最前線II 基礎と臨床の境界(田中達也編)" ライフサイエンス,東京, 167 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morimoto K 他3名: "Kindling 5" Plenum,New York, (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Onodera k.Itoh C,Sato M,Watanabe T: "Motor behavioral function for histamine-dopamine interaction in brain." Inflamm Res47 Suppl 1. S30-31. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imran MB,Kawashima R,Awata S et al.: "Use of automatized image registration of penetrate mean brain SPECT image of Alzheimer's Patients." Ann Nucl Med. 12. 127-132 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyota H,Itoh C,Osawa M et al.: "Decreased central histamine in the amygdaloid kindling rats" Brain Res. 802. 241-246 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida S,Numachi Y,Matuoka H et al.: "Impairment of cliff avoidance reaction induced by subchronic methamphetamine administration and resistant stress : comparison between two imbred strains of rats" Prog Neuro-psychopharmacol Biol Psychiat. 22. 1023-1032 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shen H,Awata S,Konno M et al.: "A lasting change in trazodone response after non-convulsive electroshock theraphy for medication-resistant senile depression" Psychiatry Clin Neurosci. 52. 111-113 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morimoto K,Sato M et al.: "Kindling" Plenum, New York. 5. (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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