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1999 Fiscal Year Final Research Report Summary

Cloning and Functional Analysis of Genes Governing Characteristics of Renal Glomerulus

Research Project

Project/Area Number 09470237
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

YAMAMOTO Tadashi  Niigata University, School of Medicine ; Professor., 医学部, 教授 (30092737)

Co-Investigator(Kenkyū-buntansha) KAWASAKI Katsutoshi  School of Medicine ; Niigata University Lecturer., 医学部, 講師 (20152944)
KIHARA Itaru  School of Medicine ; Niigata University Professor., 医学部, 教授 (80018324)
YAOITA Eishin  School of Medicine ; Niigata University Associate Professor., 医学部, 助教授 (00157950)
Project Period (FY) 1997 – 1999
KeywordsGlomerulus / Differential screening / Structure-function correlation / Ribonuclease protection assay / In situ hybridization
Research Abstract

The unique function of renal glomerulus should be related to its unique structure and molecular components. To understand the structural and functional characteristics of renal glomerulus we first aimed to identify molecules which were specifically present in this structure. We employed differential screening methods to isolate genes which were predominantly expressed in rat glomerulus. Several genes have been isolated as those which were predominantly or uniquely expressed in the glomerulus although their sequences had been registered : olfactomedin-related protein, thymosin β4, heparin-binding epidermal growth factor, VE-cadherin, βCOP and collagen type IV α3 chain. A few novel genes were also isolated as glomerulus-prone ones : 14-1 and 17-3. In addition, several novel genes were identifed as kidney-specific or kidney-prone ones : Ig-Hepta, FR-67 and CS-5. Among them, olfactomedin-related protein and Ig-Hepta were intensively studied. Olfactomedin-related protein was localized in the Golgi apparatus of glomerular podocytes and its C-terminus amino acid sequence, SDEL was a Golgi-retension signal. Ig-Hepta had two Ig-like domains and 7 transmembrane domains. The molecule was immunolocalized in the intercalated cells in the collecting duccts, suggesting a role in acid-base balance regulation.

  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] Kondo D 他: "Localization of olfactomedin-related glycoprotein isoform(BMZ) in the Golgi apparatus of podocytes in rat kidneys"J Am Soc Nephrol. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawauchi H 他: "Cloning of rat nephrin : Expression in developing glomeruli and in proteinuric states"Kidney International. -.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe J 他: "Ig-Hepta : a novel member of the G protein-coupled hepta-helical receptor(GPCR) family that has immunoglobulin-like repeats in a long N-terminal extracellular domain and defines a new subfamily of GPCRs"J Biol Chem. 274. 19957-19964 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adhikary LP 他: "Expression profile of extracellular matrix and its regulatory proteins during the process of interstitial fibrosis after anti-glomerular basement membrane antibody-induced glomerular sclerosis in SD rats"Pathol International. 49. 726-730 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kihara I 他: "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy"Histopathlogy. 34. 537-547 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yaoita E 他: "Identification of renal podocytes in multiple species : higher vertebrates are vimentin positive / lower vertebrates are desmin positive"Histochem Cell Biol. 111. 107-115 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koyama Y 他: "Expression and localization of aquaporins in rat gastrointestinal tract"Am J Physiol. 276. C261-C627 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Funaki H 他: "Immunocalization and Expression of AQP5 in Systemic Organs"Am J Physiol. 275. C1151-C1157 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto T 他: "Aquaporins in the kidney ; Emerging new aspects"kidney International. 54. 1041-1051 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chen S 他: "In vivo inhibition of CC and CX3C chemokine-induced leukocyte infiltration and attenuation of glomerulonephritis in WKY rats by vMIP-II"J Exp Med. 188. 193-198 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara M 他: "Immunohistochemical and urinary markers of podocyte injury"Pediatr Nephrol. 12. 43-48 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goto S 他: "Involvement of R-cadherin in the early stage of glomerulogenesis"J Am Soc Nephrol. 9. 1234-1241 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe A 他: "Thyroid hormone Regulates Expression of Shaker-related potassium Channel mRNA in Rat Heart"Biochem biophys Res Commun. 245. 226-230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morita T 他: "Mesangiolysis : An Update"Am J Kidney Dis. 31. 559-573 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirose S 他: "Expression and localization of cyclooxygenase isoforms and cytosolic phospholipase A2 in anti-Thy-1 glomerulonephritis"J Am Soc Nephrol. 9. 408-416 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koyama Y 他: "Molecular cloning of a new aquaporin from rat pancreas and liver"J Biol Chem. 272. 30329-30333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kihara I 他: "Podocyte detachment and epithelial cell reaction in focal segmental glomerulosclerosis with cellular variants"Kidney International. 52. S171-S176 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto 他: "Expression of AQP family in rat kidneys during the development and maturation"Am J Physiol. 272. F198-F204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujinaka H 他: "Suppression of anti-GBM nephritis by administration of anti-MCP-1 antibody in WKY rats"J Am Soc Nephrol. 8. 1174-1178 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujinaka H 他: "A crucial role of CD8-positive lymphocytes in glomerular expression of ICAM-1 and cytokines in crescentic glomerulonephriis of WKY rats"J Immunol. 158. 4978-4983 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okura Y 他: "Characterization of cytokine and iNOS mRNA expression in situ during the course of experimental autoimmune myocarditis in rats"J Mol Cell Cardiol. 29. 491-502 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishibashi K 他: "Immunolocalization and effect of dehydration on AQP3, a basolateral water channel of kidney collecting ducts"Am J Physiol. 272. F235-F241 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Endoh Y 他: "Juxtaglomerular cell tumor of the kidney : Report of a non-functioning variant"Pathology International. 47. 393-396 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokoro Y 他: "Relevance of local Th2-type cytokine mRNA expression in immunocompetent infiltrates in inflamed gingival tissue on the type of periodontal disease"Clin exp Immunol. 107. 166-174 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 格: "Annual Review腎臓:糸球体の特性や糸球体腎炎に関与する遺伝子のクローニング"中外医学社. 68-73 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 格: "内科学:糸球体疾患の成因と進展機序に関する最近の知見"医学書院(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yaoita E 他: "Cell-Cell Interaction in Kidney : Transient expression of desmoplakins in murine glomerulogenesis"Nihon-Igakkan. 29-40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawasaki K 他: "Cell-Cell Interaction in Kidney : Adhesion molecules in anti-GBM nephritis of WKY rat"Nihon-Igakkan. 46-78 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo D, Yamamoto T, Yaoita E, Danielson PE, Kobayashi H, Ohshiro F, Funaki H, Koyama Y, Fujinaka, Goto K, Kawasaki K, Sutcliffe JG, Arakawa M, Kihara I.: "Localization of olfactomedin-related glycoprotein isoform (BMZ) in the Golgi apparatus of podocytes in rat kidneys."J Am Soc Nephrol. (in press)..

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawachi H, Koike H, Yaoita E, Orikasa M, Shia MA, Yamamoto T, Salant DJ, Schimizu F.: "Cloning of rat nephrin : Expression in developing glomeruli and in proteinuric states."Kidney International. (in press)..

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe J, Suzuki H, Notoya M, Yamamoto T, Hirose S.: "Ig-Hepta : a novel member of the G protein-coupled hepta-helical receptor (GPCR) family that has immunoglobulin-like repeats in a long N-terminal extracellular domain and defines a new subfamily of GPCRs."J Biol Chem. 274. 19957-19964 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Adhikary LP, Yamamoto T, Isome M, Nakano Y, Kawasaki K, Yaoita E, Kihara I.: "Expression profile of extracellular matrix and its regulatory proteins during the process of interstitial fibrosis after anti-glomerular basement membrane antibody-induced glomerular sclerosis in SD rats."Pathol International. 49. 726-730 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kihara I, Yaoita E, Kawasaki K, Yamamoto T, Hara H, Yanagihara T.: "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy."Histopathlogy. 34. 537-547 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyama Y, Yamamoto T, Tani T, Nihei K, Kondo D, Funaki H, Yaoita E, Kawasaki K, Sato N, Harakeyama K, Kihara I.: "Expression and localization of aquaporins in rat gastrointestinal tract."Am J Physiol. 276. C261-C627 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yaoita E, Franke WW, Yamamoto T, Kawasaki K, Kihara I.: "Identification of renal podocytes in multiple species : higher vertebrates are vimentin positive / lower vertebrates are desmin positive."Histochem Cell Biol. 111. 107-115 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funaki H, Yamamoto T, Koyama Y, Kondo D, Yaoita E, Kawasaki K, Kobayashi H, Sawaguchi S, Abe H, Kihara I.: "Immunocalization and Expression of AQP5 in Systemic Organs."Am J Physiol. 275. C1151-C1157 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto T, Sasaki S.: "Aquaporins in the kidney ; Emerging new aspects."Kidney International. 54. 1041-1051 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh H, Kawasaki K, Kihara I, Nakano Y.: "Importance of type IV collagen, laminin, and heparan sulfate proteoglycan in the regulation of labyrinthine fluid in the rat cochlear duct."Eur Arch Otorhinolaryngol. 225. 285-288 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chen S, Bacon KB, Garcia G, Xia Y, Lo D, Thompson DA, Siani MA, Yamamoto T, Harrision JK, Feng L.: "In vivo inhibition of CC and CX3C chemokine-induced leukocyte infiltration and attenuation of glomerulonephritis in WKY rats by vMIP-II."J Exp Med. 188. 193-198 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara M, Yanagihara T, Itoh M, Matsuno M, Kihara I.: "Immunohistochemical and urinary markers of podocyte injury."Pediatr Nephrol. 12. 43-48 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto S, Yaoita E, Matsunami H, Kondo D, Yamamoto T, Kawasaki K, Arakawa M, Kihara I.: "Involvement of R-cadherin in the early stage of glomerulogenesis."J Am Soc Nephrol. 9. 1234-1241 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita N, Kimura A, Matsuki Y, Yamamoto T, Yoshie H, Hara K.: "Activation of transcription factors and IL-8 expression in neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis."Inflammation. 22. 253-267 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yagi M, Yamamoto T, Kato S, Nagano N, Kihara I.: "Long-term observation of glomerulonephritis induced by multiple injections with anti-Thy-1 antibody in rats."Pathol International. 48. 491-498 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe A, Yamamoto T, Isome M, Ma M, Yaoita E, Kawasaki K, Kihara I, Aizawa Y.: "Thyroid hormone Regulates Expression of Shaker-related potassium Channel mRNA in Rat Heart."Biochem Biophys Res Commun. 245. 226-230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita T, Yamamoto T, Churg J.: "Mesangiolysis : An Update."Am J Kidney Dis. 31. 559-573 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose S, Yamamoto T, Feng T, Yaoita E, Kawasaki K, Goto S, Fujinaka H, Wilson CB, Arakawa M, Kihara I.: "Expression and localization of cyclooxygenase isoforms and cytosolic phospholipase A2 in anti-Thy-1 glomerulonephritis."J Am Soc Nephrol. 9. 408-416 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara M, Yanagihara T, Takada T, Itoh M, Matsuno M, Yamamoto T, Kihara I.: "Urinary excretion of podocytes reflects disease activity in children with glomerulonephritis."Am J Nephrol. 18. 35-41 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyama Y, Yamamoto T, Kondo D, Funaki H, Yaoita E, Kawasaki K, Sato N, Hatakeyama K, Kihara I.: "Molecular cloning of a new aquaporin from rat pancreas and liver."J Biol Chem. 272. 30329-30333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kihara I, Tsuchida S, Yaoita E, Yamamoto T, Hara M, Yanagihara T, Takada T.: "Podocyte detachment and epithelial cell reaction in focal segmental glomerulosclerosis with cellular variants."Kidney International. 52. S-171-S-176 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto T, Sasaki S, Fushimi K, Ishibashi K, Yaoita E, Kawasaki K, Fujinaka H, Marumo F, Kihara I.: "Expression of AQP family in rat kidneys during the development and maturation."Am J Physiol. 272. F198-F204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujinaka H, Yamamoto T, Takeya M, Feng L, Kawasaki K, Yaoita E, Kondo D, Wilson CB, Uchiyama M, Kihara I.: "Suppression of anti-GBM nephritis by administration of anti-MCP-1 antibody in WKY rats."J Am Soc Nephrol. 8. 1174-1178 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujinaka H, Yamamoto T, Feng L, Kawasaki K, Yaoita E, Hirose S, Goto S, Wilson CB, Uchiyama M, Kihara I.: "A crucial role of CD8-positive lymphocytes in glomerular expression of ICAM-1 and cytokines in crescentic glomerulonephritis of WKY rats."J Immunol. 158. 4978-4983 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okura Y, Yamamoto T, Goto S, Inomata T, Hanawa H, Feng L, Wilson CB, Izumi T, Shibata A, Kihara I, Seki S, Abo T.: "Characterization of cytokine and iNOS mRNA expression in situ during the course of experimental autoimmune myocarditis in rats."J Mol Cell Cardiol. 29. 491-502 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishibashi K, Sasaki S, Fushimi K, Yamamoto T, Kuwahara M, Marumo F.: "Immunolocalization and effect of dehydration on AQP3, a basolateral water channel of kidney collecting ducts."Am J Physiol. 272. F235-F241 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokoro Y, Matsuki T, Yamamoto T, Suzuki T, Hara K.: "Relevance of local Th2-type cytokine mRNA expression in immunocompetent infiltrates in inflamed gingival tissue on the type of periodontal disease."Clin exp Immunol. 107. 166-174 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi H, Orikasa M, Naito M, kawasaki K, Oite T, Kawachi H, Yamashita A, Takeya M, Nihei H, Shimizu F.: "Detailed analysis of phenotypes of macrophages infiltrating glomeruli in rat anti-Thy-1 nephritis."Nephron. 77. 333-339 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Endoh Y, Motoyama T, Hayami S, Kihara I.: "Juxtaglomerular cell tumor of the kidney : Report of a non-functioning variant."Pathology International. 47. 393-396 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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