1999 Fiscal Year Final Research Report Summary
DIMINISHED CYTOTOXIC ANTI-DONOR ANTIBODIES IN RAT HEART TRANSPLANTATION WITH TREATMENT OF ANTI-B-CELL MONOCLONAL ANTIBODY
| Project/Area Number |
09470279
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Department of Surgery II, Ehime University School of Medicine |
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Principal Investigator |
KAWACHI Kanji Department of Surgery II, Ehime University School of Medicine, Professor, 医学部, 教授 (90116020)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tetsuya Department of Surgery II, Ehime University School of Medicine, Lecturer, 附属病院, 助手 (70240108)
HAMADA Yoshihiro Department of Surgery II, Ehime University School of Medicine, Lecturer, 医学部, 助手 (90218545)
KASHU Yasuaki Department of Surgery II, Ehime University School of Medicine, Lecturer, 医学部, 助手 (40294813)
NAKATA Tatsuhiro Department of Surgery II, Ehime University School of Medicine, Lecturer, 附属病院, 助手 (40260690)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Heart Transplantation / Rat. / B-cell / Humoral immunity / Monoclonal Antibody |
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| Research Abstract |
Background : T-cell mediated immunity plays an important role in acute allograft rejection ; however, the role of B-cell-dependent antibody-producing reactions in the rejection process is unclear. Methods : In this study, anti-B-cell monoclonal antibody Rub-1 was given to rats after heart allograft transplantation, and graft survivals and changes in antibodies to donor specific cells were investigated. Results : Control heart grafts (heterotopic heart transplantation without any immunosuppressive treatment) were rejected by 6.4 days. However, heart allograft survival was prolonged to 15.4 days after transplantation when treated with Rub-1 for 5 days after grafting. The elevation of anti-allo-specific lymphocytotoxic antibodies was markedly suppressed in the Rub-1-treated host rats. Conclusions : Cytotoxic anti-donor antibodies play an important role in acute heart allograft rejection. Suppression of B cell function using anti-rat B-cell monoclonal antibodies can be a supplemental immunosuppressive therapy after heart allo-transplantation.
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