1998 Fiscal Year Final Research Report Summary
Analysis of cell death inducing gene in human malignant gliomas
| Project/Area Number |
09470292
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
YAMASHITA Junkoh Department of Neurosurgery Kanazawa University Chief Proffesor, 医学部, 教授 (90026948)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Osamu Department of Neurosurgery Kanazawa University Assistant Proffesor, 医学部, 助手 (40211362)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Keywords | glioma / apoptosis, / Caspase-3 |
|---|
| Research Abstract |
Cysteine proteases of caspase family (interleukin-1 beta -converting enzyme) have been implicated as components of cell death pathway and have been reported to involved in Fas, chemotherapeutic agents, and radiation-induced apoptosis. In this study, I assessed the expression of Caspase-1, Caspase-2 and Caspase-3 in 11 cases of primary astrocytic tumors ( five anaplastic astrocytomas, and six glioblastomas) by reverse transcription (RT)-PCR, Western blot analysis, immunohistochemistry, and in situ casspase-3 activity assay. The frequency of Caspase-1, Caspase-2 and Caspase-3 overexpression appears to correlate with the malignancy grade of astrocytic brain tumors. Furthermore, Caspase-2 and Caspase-3 overexpression and Caspase-3 activation may play an important role in the pathogenesis of necrosis, which is one of the histological hallmarks of glioblastoma.
|
