1999 Fiscal Year Final Research Report Summary
Analysis of characterization of a GC factor in malignant brain tumor and its application to the gene therapy
| Project/Area Number |
09470296
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
KURISU Kaoru Hiroshima University, Faculty of Medicine, Professor, 医学部, 教授 (70201473)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Kazuhiko Hiroshima University, Medical Hospital, Assistant Professor, 医学部・附属病院, 講師 (30243554)
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| Project Period (FY) |
1997 – 1999
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| Keywords | GC factor / malignant glioma / EGFR / adenovirus / cell cycle / NBS1 / telomerase |
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| Research Abstract |
GC factor (GCF) is a human transcriptional regulator that binds to specific GC-rich sequences in the certain genes promoter and represses its transcription. Malignant glioma express multiautocrine loops of growth factor/receptor, especially overexpress the epidermal growth factor receptor (EGFR). We investigated the expression of the EGFR in glioma cell lines. By the use of GCF transfection, we examined whether GCF represses the expression of growth factors and receptors and cause growth inhibition of glioma cells. The transfection of GCF expression vector into glioma cell line (U251-MG) decreased the protein level of EGFR and insulin-like growth factor-II (IGF-II). We also examined the effect of GCF gene transfer on the chemosenstivity to ACNU, CDDP and VCR. The slight increase of sensitivity to ACNU. CDDP and VCR was found. We developed a recomblnant adenovirus expressing GCF gene (AxCA-GCF) by COS-TPC method. We are investigating the growth inhibition of glioma by infection of AxCA-GCF and in vitro and in vivo.
Also, we investigated the molecular changes of p16 gene, NBS1, telomerase activity in human gliomas and malignant lymphoma. And we studied p16 gene transfection effects on growth of glioma cells and senstivity of cells to ACNU, CDDP and AZC.
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Research Products
(19 results)
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[Publications] Seiji Hama, Shinya Matsuura, Hiroshi Tauchi, Jyunko Sawada, Chikage Kato, Fumiyuki Yamasaki, Hiroyuki Yoshioka, Kazuhiko Sugiyama, Kazunori Arita, Kaoru Kurisu, Nakano Kamada, Yuji Heike, Keishi Komatsu: "Absence of mutations in the NBS1"Anticancer research. (in press). (2000)
Description
「研究成果報告書概要(欧文)」より
