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1999 Fiscal Year Final Research Report Summary

Study on the role of lymphocytes on organ failure and disturbed host defense mechanism due to infection and stress

Research Project

Project/Area Number 09470324
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionAKITA UNIVERSITY

Principal Investigator

INABA Hideo  School of Med., Akita Univ., Professor, 医学部, 教授 (60159952)

Co-Investigator(Kenkyū-buntansha) NAKAE Hajime  School of Med., Akita Univ., Research Assoc., 医学部, 助手 (10254781)
KUROSAWA Shin  School of Med., Tohoku Univ., Research assoc., 医学部, 助手 (60272043)
MOTOYAMA Satoru  School of Med., Akita Univ., Research Assoc., 医学部, 助手 (60292372)
TANAKA Hiroyuki  School of Med., Akita Univ., Assoc. Prof., 医学部, 助教授 (90266297)
MACHII Masato  School of Med., Akita Univ., Research Assoc., 医学部, 助手 (50209467)
Project Period (FY) 1997 – 1999
Keywordslymphocyte / apoptosis / hydrogen peroxide / hepatocytes / endothelial cells / heat shock protein / bcl-2 / ischemia
Research Abstract

Lympocytes may induce acute injuries of the perfused liver when sinusoidal endothelial cell apoptosis is induced by the administration of OK432 or ischemic challenge, and when lymphocytes are activated by concanavalin A. In the schemic liver, hydrogen peroxide derived from hepatocytes induces sisusoidal endothelial cell apoptosis in the midzone of Liver acinus as well as Hsp 72 expression in sinusoidal endothelial cells. An anti-apoptotic protein Bcl-2 is located in the inner membrane and crista of mitochondria in the hepatocytes. Localization of Bcl-2 is unchanged by the ischemic challenge.
In a mouse model of severe infection and sepsis induced consequent administrations of Ok432 and lipopolysaccharide, both natural killer and lymphokine-activated killer activities are elevated, but lymphocytes are very sensitive to Fas-mediated apoptosis.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Motoyama S.Inaba H.et al.: "Hydrogen peroxide derived from hepatocytes induces sinusoidal endothelial cell apoptosis in perfused hypoxic rat liver"Gastoroenterology. 114. 153-163 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyama S.Inaba H.et al.: "Bcl-2 is located predominantly in the inner membrane and crista of mitochondria in rat liver"Biochem Biophys Res Commun. 249. 628-636 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyama S.Nakae H.Inaba H.et al.: "Red blood cells atlenuate sinusoidul endothelial cell injury by scavenging xanthine oxidase-dependent hydrogen peroxide"Liver. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyama S.Inaba H.Nakae H et al.: "Hydrogen peroxide induces midzonal heat shock protein 72 apoptosis in sinusoidal endothelial cells of perfused rat liver"Crit Care Med.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyama S.Inaba H.: "Mitochondrial Ubiquinone (coenzymeQ10) : Biochemical functional, medical and therapeutic aspect in human health"Academic Press (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Motoyama S, Inaba H, et al.: "Hydrogen peroxide derived from hepatocytes induces sinusoidal endothelial cell apoptosis in perfused hypoxic rat liver."Gastroenterology. 114. 153-163 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoyama S, Inaba H. et al.: "Bcl-2 is located predominantly in the inner membrane and crista of mitochondria in rat liver."Biochem Biophys Res Commun. 249. 628-636 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoyama S, Inaba H. et al.: "Hydrogen peroxide induces midzonal heat shock protein 72 apoptosis in sinusoidal endothelial cells of hypoperfused rat liver."Crit Care Med. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoyama S, Inaba H, Nakae H, et al.: "Red blood cells attenuate sinusoidal endothelial cell injury by scavenging xanthine oxidase-dependent hydrogen."Liver. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motoyama S, Inaba H, et al.: "Bcl-2 is located predominantly in the inner membrane and crista of mitochondria"

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] "Motochondrial Ubiquinone (Coenzyme Q10): Biochemical, functional, medical, and therapeutic aspect in human health and disease."Ed. Eboadi M, Marwash J, Chopra RK: Academic Press in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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