1998 Fiscal Year Final Research Report Summary
Identification of metastasis suppressor genes for prostate cancer and analysis of genetic changes during progression of prostate cancer.
| Project/Area Number |
09470340
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | CHIBA UNIVERSITY (1998)
Teikyo University (1997) |
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Principal Investigator |
ICHIKAWA Tomohiko Chiba University, University Hospital, Lecturer, 医学部・附属病院, 講師 (20241953)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Prostate cancer / Metastasis / Metastasis suppressor genes / Gene cloning |
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| Research Abstract |
Identification of molecular and cellular markers for the metastatic ability of prostate cancer would be useful in developing diagnostic methods for substaging histologically localized prostate cancers. To isolate metastasis suppressor genes, we have established a rat prostate cancer system. In this system, we used a microcell mediated chromosome transfer technique to introduce a single copy of normal human chromosomes into highly metastatic rat prostate cancer.
In the previous studies, we isolated KAI1/CD82, a metastasis suppressor gene for prostate cancer, from 11p11.2. We also demonstrated that KAI1/CD82 was down- regulated during the progression of human prostate cancer. In the present study, we demonstrate that this down regulation does not require either deletion or point mutation of KAI1/CD 82.
By using the same method, we reported that another metastasis suppressor gene was located on the human chromosome region 8p21-p12. In the present study, this region has been narrowed down to about 1 cM.This is overlapped to the regon where loss of heterozygosity (LOH) has been observed frequently in human prostate cancer We are currently constructing a BAC contig map that covers this 1 cM region.
We have also published that human chromosomes 7 and 12 contain metastasis suppressor genes for prostate cancer. We have also published that human chromosome 19 contains a tumor suppressor gene for prostate cancer.
Human chromosome 13 reduces metastatic ability without suppression of tumorigenicity of rat prostate cancer. We have performed LOU analysis in the human prostate cancer and have shown that the 13q14 is associated with metastasis, which has been published.
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[Publications] Kuramochi, H., Ichikawa, T., Nihei, N., Kawana, Y., Suzuki, H., Schalken, J.A., Takeichi, M., Nagafuchi, A., and Shimazaki, J.: "Suppression of invasive ability of highly metastatic rat prostate cancer by introduction of human chromosome 8." Prostate. 31(1). 14-20 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Kawana, Y., Komiya, A., Ueda, T., Nihei, N., Kuramochi, H., Suzuki, H., Yatani, R., Imai, T., Dong, J-T., Imai, T., Yoshie, O., Barrett, J.C., Isaacs, J.T., Shimazaki, J., Ito.H., and Ichikawa, T.: "Location of KAI1 on the short arm of human chromosome 11 and frequency of allelic loss in advanced human prostate cancer." Prostate. 32(3). 205-213 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Luu, H.H., Zagaja, G.P., Dubauskas, Z., Chen, S.L., Smith, R.C., Watabe, K., Ichikawa, T., Davis, E.M., Le Beau, M.M., and Rinker-Schaeffer, C.W.: "Identification of a novel metastasis-suppressor region on human chormosome 12." Cancer Res.58(16). 3561-3565 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Nihei, N., Ohta, S., Kuramochi, H., Kugoh, H., Oshimura, M., Barrett, J.C., Isaacs, J.T., Ito, H., Masai, M., Ichikawa, Y., and Ichikawa, T.: "Metastasis suppressor gene(s)for rat prostate cancer on the long arm of human chromosome 7." Genes Chromosomes Cancer. 24(1). 1-8 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Gao, A.C., Lou, W., Ichikawa, T., Denmeade, S.R., Barrett, J.C., and Isaacs, J.T.: "Suppression of the tumorigenicity of prostatic cancer cells by gene(s)located on human chromosome 19p13.1-13.2." Prostate. 38(1). 46-54 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Ueda, T., Emi, M., Suzuki, H., Komiya, A., Akakura, K., Ichikawa, T., Shiraishi, T., Masai, M., Igarashi, T., and Ito, H.: "Identification of a 1-cM region of common deletion on 13q14 associated with metastasis of human prostate cancer." Genes Chromosomes Cancer. 24(2). 183-193 (1999)
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「研究成果報告書概要(欧文)」より
