| Project/Area Number |
09470345
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyoto University |
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Principal Investigator |
FUJITA Jun Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (50173430)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Takashi Kansai Medical School, Faculty of Medicine, Professor, 医学部, 教授 (20192338)
HIGASHITSUJI Hiroaki Kyoto University, Graduate School of Medicine, Instructor, 医学研究科, 助手 (60281094)
KANEKO Yoshiyuki Kyoto University, Graduate School of Medicine, Instructor, 医学研究科, 助手 (40252465)
ITOH Katsuhiko Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (90281097)
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| Project Period (FY) |
1997 – 1998
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| Keywords | heat shock / testis / infertility / cold shock / apg-1 / apg-2 / Cirp / rbm3 |
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| Research Abstract |
1. We aimed to identify genes whose expression levels are different in the testis at 32 and 370。C.We identified a novel heat shock protein (hsp)l 10-related gene, apg-1, that is constitutively expressed in the mouse testiculr germ cells, and responds to a low temperature heat shock (shift from 32 to 390。C) rather than the traditional elevated temperatures (from 37 to 420。C) in somatic cells. By contrast, a novel apg-1 related gene apg-2 was not induced by either heat shock.
2. We have isolated the first mammalian cold shock protein, cirp from the mouse testis cDNA library. Cirp is an RNA-binding protein, induced in all somatic cells examined by a temperature shift from 37 to 320。C.It is constitutively expressed in the mouse and human germ cells in the testes, and the levels are decreased in the cryptorchid testes and those with varicocele. Cirp has an ability to inhibit progression of the mitotic cell cycle from G1 to S.
3. We showed tha human RBM3 which is structurally similar to cirp is also a cold shock protein. We isolated mouse rbm3 which was expressed in the Sertoli cells of the testis.
4. By examining various virus and mammalian promoters for expression in the murine male germ cell lines, we identified a hybrid retrovirus LTR as suitable for use.
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