1999 Fiscal Year Final Research Report Summary
BASIC AND CLINICOPATHOLOGICAL ANALYSIS FOR MOLECULES REGULATING INVASIVE AND METASTATIC PROPERTIES OF GENITOURINARY TUMORS
| Project/Area Number |
09470349
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kagoshima University |
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Principal Investigator |
SHIRAHAMA Tsutomu KAGOSHIMA UNIVERSITY HOSPITAL, DEPARTMENT OF MEDICINE, ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (90136862)
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| Co-Investigator(Kenkyū-buntansha) |
AKIBA Suminori KAGOSHIMA UNIVERSITY, DEPARTMENT OF MEDICINE, PROFESSOR, 医学部, 教授 (50145554)
KAWAHARA Motoshi KAGOSHIMA UNIVERSITY HOSPITAL, DEPARTMENT OF MEDICINE, ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (40161351)
FURUKAWA Tatsuhiko KAGOSHIMA UNIVERSITY, DEPARTMENT OF MEDICINE, RESEARCH ASSOCIATE, 医学部, 助手 (40219100)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Bladder cancer / Metanestin / Thymidine phosphorylase / VEGF / Cyclooxygenase / Testicular tumor / Renal cell carcinima |
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| Research Abstract |
(1) Identification of metastasis-associated molecule in bladder cancer. A 60-kDa glycoprotein named metanestin was identified by molecular cloning. The glycoprotein had a twice-repeated motif of Pro-Gly-Pro-Gly and carried metastasis-associated carbohydrate epitopes. The antibody to the protein portion of metanestin strongly reacted with lymph node metastasis of transitional cell carcinoma of the urinary bladder, but the reactivity to the primary tumor was generally weaker and the normal urothelium was scarcely reactive. Thus both metanestin and the carbohydrate on it showed metastasis-associated expression. In addition, we identified a l00-kDa glycoprotein with a 4-times-repeated motif of Pro-Ala-Pro-Ala. The antibody to the 100-kDa glycoprotein showed reactivity similar to that to metanestin.
(2) Clinical significance of angiogenesis factors in urogenital tumors. The enzymatic activity of thymidine phosphorylase as an angiogenesis factor and the protein expression using immunoblot and immnunohistochemical analysis were examined in tumors from kidney bladder and testis. Multivariate analysis revealed that thymidine phosphorylase was an independent prognostic factor in both renal cell and bladder carcinomas. However, it was not expressed in testicular tumor and VEGF found to be a predicting factor for lymph node metastasis by multivariate analysis.
(3) Clinical significance of cyclooxygenase-2 in bladder cancer Cyclooxygenase-2 was frequently expressed in invasive bladder tumors than in superficial bladder tumors. It was also frequently expressed in carcinoma in situ lesion, which is precursor of invasive bladder tumor. The results suggest that cylooxygenase-2 is involved in the development of bladder cancer.
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